pBABE-hygro

价格:1250元

联系方式:I47-825O-882O

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载体基本信息

出品人: Bob Weinberg
载体名称: pBABE-hygro ; pBABE hygro
质粒类型: 逆病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 5578 bp
5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #1765
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pBABE-hygro载体图谱



pBABE-hygro多克隆位点

载体简介

载体序列

LOCUS       pBABE-hygro	5578 bp 	DNA	SYN
DEFINITION  pBABE-hygro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="pBABE-hygro"
                     /mol_type="other DNA"
     promoter        complement(4..32)
                     /label="AmpR_promoter"
     misc_feature    102..569
                     /label="5_LTR2"
     misc_feature    108..569
                     /label="3MoMuLV_LTR"
     misc_feature    639..1440
                     /label="psi_plus_pack"
     misc_feature    1408..1424
                     /label="pBABE_5_primer"
     misc_feature    complement(1498..1518)
                     /label="pBABE_3_primer"
     misc_feature    complement(1504..1883)
                     /label="SV40_enhancer"
     promoter        1516..1784
                     /label="SV40_promoter"
     rep_origin      1683..1760
                     /label="SV40_origin"
     CDS             complement(1744..2664)
                     /label="ORF frame 2"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ
                     AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*"
     misc_feature    1745..1764
                     /label="SV40pro_F_primer"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ
                     AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*"
     CDS             1906..2931
                     /label="ORF frame 1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            1921..2928
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(4310..5540)
                     /label="Ampicillin (w/ gaps)"
                     /gene="Ampicillin (w/ gaps)"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             complement(4680..5540)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
   61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
  121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
  181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
  241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
  301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
  361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
  421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
  481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
  541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
  601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
  661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
  721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
  781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
  841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
  901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
  961 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
 1021 tagggccaga ctgttaccac tcccttaagt ttgaccttag atcactggaa agatgtcgag
 1081 cggctcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
 1141 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
 1201 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
 1261 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
 1321 gtacacccta agcctccgcc tcctcttctt ccatccgcgc cgtctctccc ccttgaacct
 1381 cctctttcga ccccgcctca atcctccctt tatccagccc tcactccttc tctaggcgcc
 1441 ggccggatcc cagtgtggtg gtacgtagga attcgccagc acagtggtcg accctgtgga
 1501 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
 1561 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
 1621 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
 1681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
 1741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
 1801 gaggcttttt tggaggccta ggcttttgca aaaagcttgc cagctggggc gccctctggt
 1861 aaggttggga agccctgcag atctgatccc ggggggcaat gagatatgaa aaagcctgaa
 1921 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
 1981 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
 2041 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
 2101 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga atttagcgag
 2161 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
 2221 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
 2281 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
 2341 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
 2401 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
 2461 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
 2521 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
 2581 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
 2641 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
 2701 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
 2761 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
 2821 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
 2881 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gagtagatgc
 2941 cgaccgggat ctatcgatgc ggcgggtgtg gtggttacgc gtaatctgct gcttgcaaac
 3001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
 3061 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
 3121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
 3181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
 3241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
 3301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
 3361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
 3421 aggagagcgc acgagggagc ttccagggga aacgcctggt atcttatagt cctgtcgggt
 3481 ttcgccacct ctgacttgag cgtcgatttt gtgatgctcg tcaggggggc ggagcctatg
 3541 gaaaaacgcc agcaacgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg
 3601 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
 3661 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
 3721 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
 3781 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
 3841 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
 3901 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
 3961 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
 4021 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
 4081 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
 4141 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc
 4201 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
 4261 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
 4321 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
 4381 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc ccaaggcggt
 4441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
 4501 gcaaaaggcc aggaaccgta aaaaggccgc gttacgctca gtggaacgaa aactcacgtt
 4561 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
 4621 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
 4681 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
 4741 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
 4801 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
 4861 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
 4921 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
 4981 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
 5041 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
 5101 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
 5161 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
 5221 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
 5281 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
 5341 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
 5401 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
 5461 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
 5521 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagg

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