| 质粒名称: | pRL1063a |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 70687 |
| 存储实验室: | Peter Wolk |
|
载体骨架基本信息 |
|
| 载体名称: | pRL1058 |
| 载体出品公司: | Wolk Lab |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 8939bp中的7835bp是一个转座子，其末端由转移起点连接 |
| 载体类型: | -- |
| 筛选标记: | 博莱霉素 |
| 载体抗性: | 卡那霉素、链霉素、博莱霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | Transposon Tn5-1063,pRL1063a as a whole is presented in GenBank: U55385.2 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pRL1063a was a gift from Peter Wolk (Addgene plasmid # 70687 ; http://n2t.net/addgene:70687 ; RRID:Addgene_70687) |
| 参考文献部分: | Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium. Wolk CP, Cai Y, Panoff JM. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9. PubMed 11607193 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRL1063a 8939 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pRL1058 with Vibrio fischeri luxAB for sensing.
ACCESSION .
VERSION .
KEYWORDS pRL1063a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8939)
AUTHORS Wolk CP, Cai Y, Panoff JM
TITLE Use of a transposon with luciferase as a reporter to identify
environmentally responsive genes in a cyanobacterium.
JOURNAL Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5355-9.
PUBMED 11607193
REFERENCE 2 (bases 1 to 8939)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8939
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 2538..3082
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 2983..3002
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3199..3216
/label=L4440
/note="L4440 vector, forward primer"
CDS 3449..4243
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3503..3522)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4113..4132
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
CDS complement(8100..8471)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 8504..8613
/note="incP origin of transfer"
ORIGIN
1 ctgactctta tacacaagta gcgtcctgaa cggaaccttt cccgttttcc aggcgggatc
61 ctctagagga tccccgggta ccgagctcat tgatagcatt gaatctagta attgtaagct
121 ctattcgcta attggaagtt cacatgatct tggggaaaat ttggttgtat taagaaattt
181 ttatcaatca gtgacgaagg cagccttagc attagatgat ggtttattgg atttagagat
241 agacattatt gaacctcgat ttgaggacgt tacaagtatt actgttaagg agcgtagatt
301 aaaaaatgaa attgaaaatg aattattaga attggcttaa ataaacagaa tcaccaaaaa
361 ggaatagagt atgaagtttg gaaatatttg tttttcgtat caaccaccag gtgaaactca
421 taagcaagta atggatcgct ttgttcggct tggtatcgcc tcagaagagg tagggtttga
481 tacatattgg accttagaac atcattttac agagtttggt cttacgggaa atttatttgt
541 tgctgcggct aacctgttag gaagaactaa aacattaaat gttggcacta tgggggttgt
601 tattccgaca gcacacccag ttcgacagtt agaagacgtt ttattattag atcaaatgtc
661 gaaaggtcgt tttaattttg gaaccgttcg agggctatac cataaagatt ttcgagtatt
721 tggtgttgat atggaagagt ctcgagcaat tactcaaaat ttctaccaga tgataatgga
781 aagcttacag acaggaacca ttagctctga tagtgattac attcaatttc ctaaggttga
841 tgtatatccc aaagtgtact caaaaaatgt accaacctgt atgactgctg agtccgcaag
901 tacgacagaa tggctagcaa tacaagggct accaatggtt cttagttgga ttattggtac
961 taatgaaaaa aaagcacaga tggaactcta taatgaaatt gcgacagaat atggtcatga
1021 tatatctaaa atagatcatt gtatgactta tatttgttct gttgatgatg atgcacaaaa
1081 ggcgcaagat gtttgtcggg agtttctgaa aaattggtat gactcatatg taaatgcgac
1141 caatatcttt aatgatagca atcaaactcg tggttatgat tatcataaag gtcaatggcg
1201 tgattttgtt ttacaaggac atacaaacac caatcgacgt gttgattata gcaatggtat
1261 taaccctgta ggcactcctg agcagtgtat tgaaatcatt caacgtgata ttgatgcaac
1321 gggtattaca aacattacat gcggatttga agctaatgga actgaagatg aaataattgc
1381 ttccatgcga cgctttatga cacaagtcgc tcctttctta aaagaaccta aataaattac
1441 ttatttgata ctagagataa taaggaacaa gttatgaaat ttggattatt ttttctaaac
1501 tttcagaaag atggaataac atctgaagaa acgttggata atatggtaaa gactgtcacg
1561 ttaattgatt caactaaata tcattttaat actgcctttg ttaatgaaca tcacttttca
1621 aaaaatggta ttgttggagc acctattacc gcagctggtt ttttattagg gttaacaaat
1681 aaattacata ttggttcatt aaatcaagta attaccaccc atcaccctgt acgtgtagca
1741 gaagaagcca gtttattaga tcaaatgtca gagggacgct tcattcttgg ttttagtgac
1801 tgcgaaagtg atttcgaaat ggaatttttt agacgtcata tctcatcaag gcaacaacaa
1861 tttgaagcat gctatgaaat aattaatgac gcattaacta caggttattg tcatccccaa
1921 aacgactttt atgattttcc aaaggtttca attaatccac actgttacag tgagaatgga
1981 cctaagcaat atgtatccgc tacatcaaaa gaagtcgtca tgtgggcagc gaaaaaggca
2041 ctgcctttaa catttaagtg ggaggataat ttagaaacca aagaacgcta tgcaattcta
2101 tataataaaa cagcacaaca atatggtatt gatatttcgg atgttgatca tcaattaact
2161 gtaattgcga acttaaatgc tgatagaagt acggctcaag aagaagtgag agaatactta
2221 aaagactata tcactgaaac ttaccctcaa atggacagag atgaaaaaat taactgcatt
2281 attgaagaga atgcagttgg gtctcatgat gactattatg aatcgacaaa attagcagtg
2341 gaaaaaacag ggtctaaaaa tattttatta tcctttgaat caatgtccga tattaaagat
2401 gtaaaagata ttattgatat gttgaaccaa aaaatcgaaa tgaatttacc ataataaaat
2461 taaaggcaat ttctatatta gattgccttt ttggggatcc tctagaaata ttttatctga
2521 ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
2581 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac
2641 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
2701 ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg
2761 tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
2821 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
2881 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
2941 accgaaaggc aggaacagga gagcgcacga gggagccgcc aggggaaacg cctggtatct
3001 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
3061 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt
3121 atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc
3181 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac
3241 atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc acttcactga
3301 caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagagga tctcaatgaa
3361 tattggttga cacgggcgta taagacatgt tatactgttg aataacaagg acggatctga
3421 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3481 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3541 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
3601 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
3661 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
3721 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
3781 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
3841 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
3901 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
3961 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
4021 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
4081 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4141 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4201 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
4261 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
4321 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
4381 cgcggggatc tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg
4441 ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc
4501 ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg
4561 ggaggcacga tggccgcttt ggtcgacccg gacgggacgc tcctgcgcct gatacagaac
4621 gaattgcttg caggcatctc atgagtgtgt cttcccgttt tccgcctgag gtcactgcgt
4681 ggatggagcg ctggcgcctg ctgcgcgacg gcgagctgct caccacccac tcgagctgga
4741 tacttcccgt ccgccagggg gacatgccgg cgatgctgaa ggtcgcgcgc attcccgatg
4801 aagaggccgg ttaccgcctg ttgacctggt gggacgggca gggcgccgcc cgagtcttcg
4861 cctcggcggc gggcgctctg ctcatggagc gcgcgtccgg ggccggggac cttgcacaga
4921 tagcgtggtc cggccaggac gacgaggctt gcaggatcct ctgcgacacc gccgctcgtc
4981 tgcacgcgcc gcggtccgga ccgccgcccg atctccatcc gctacaggaa tggttccagc
5041 cgcttttccg gttggccgct gagcacgcgg cacttgcgcc cgccgccagc gtagcgcgcc
5101 aacttctggc ggcgccgcgc gaggtgtgcc cgctccacgg cgacctgcac cacgagaacg
5161 tgctcgactt cggcgaccgc ggctggctgg ccagcgaccc gcacggactg ctcggcgagc
5221 gcaccttcga ctatgccaac atcttcacga atcccgatct cagcgacccc ggtcgcccgc
5281 ttgcgatcct gccgggcagg ctggaggctc gactcagcat tgtggtcgcg acgaccgggt
5341 ttgagcccga acggcttctt cgctggatca ttgcatggac gggcttgtcg gcagcctggt
5401 tcatcggcga cggcgacggc gagggcgctg cgattgatct ggccgtaaac gccatggcac
5461 gccggttgct tgactagcgc ggtcaccgat ctcacctggt cgtcgagcta ggtcaggccg
5521 tgtcgggcgt gatccgctgg aagtcgttgc gggccacacc cgccgcctcg aagccctgca
5581 ccaggccggc atcgtggtgt gcgtggccga gggactatgg aaggtgccgg acgatctgcc
5641 cgagcagggc cgccgctatg acgcccggga gtcagcgcag aatggtgaac cagagcaagg
5701 gaatatgtta cgcgtgacgc agtagcggta aacggcagac aaaaaaatgt cgcacaatgt
5761 gcgccatttt tcacttcaca ggtggaattg tacccttggt ggcgtgacgg tggagctgaa
5821 atcgcacctg cccatcgagc ggcaggcccg cgtgatcggt gccacctggc ttgaccagca
5881 gttgatcgac ggtggctcgg gcttgggcga cctgggcttt agcagtgagg ccaagtaggc
5941 gatacagcag cgcgcggact tcctggccga acagggactg gccgagcggc gcgggcagcg
6001 cgtgatcctc accggaatct gctcggcagc agcgggctcg ggaactggcg caggccgcga
6061 aggacattgc cgccgatacc ggcctggagc atcgccccgt ggccgacggc cagcgcgttg
6121 ccggcgtcta ccggcgcccc gtcatgctcg ccagcgggcg aaatgggatg cttgatgacg
6181 ccaaggggtc cagcctcgtg cggtggaagc ccatcgaaca gcggcttggg gagcagctcg
6241 ccgcgacggt gcgcggtggc ggcgtgtctt gggagattgg acgacagcgt gggccggccc
6301 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
6361 tccagtttac tttgcagggc ttcccaacct tcccagaggg cgccccagct ggcaattccg
6421 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
6481 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
6541 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
6601 agcccttgcg ccctgagtgc ttgcggcagc gtgaagcttt ctctgagctg taacagcctg
6661 accgcaacaa acgagaggat cgagaccatc cgctccagat tatccggctc ctccatgcgt
6721 tgcctctcgg ctcctgctcc ggttttccat gccttatgga actcctcgat ccgccagcga
6781 tgggtataaa tgtcgatgac gcgcaaggct tgggctagcg actcgaccgg ttcgccggtc
6841 agcaacaacc atttcaacgg ggtctcaccc ttgggcgggt taatctcctc ggccagcacc
6901 gcgttgagcg tgatattccc ctgttttagc gtgatgcgcc cactgcgcag gctcaagctc
6961 gccttgcggg ctggtcgatt tttacgttta ccgcgtttat ccaccacgcc cttttgcgga
7021 atgctgatct gatagccacc caactccggt tggttcttca gatggtcgat cagatacaac
7081 ccagactcta cgtccttgcg tgggtgcttg gagcgcacca cgaagcgctc gttatgcgcc
7141 agcctgtcct gcagataagc atgaatatcg gcttcgcggt cacagaccgc aatcacgttg
7201 ctcatcatgc tgcccatgcg taaccggcta gttgcggccg ctgccagcca tttgccactc
7261 tccttttcat ccgcatcggc agggtcatcc gggcgcatcc accactcctg atgcagtaat
7321 cctacggtgc ggaatgtggt ggcctcgagc aagagaacgg agtgaaccca ccatccgcgg
7381 gatttatcct gaatagagcc cagcttgcca agctcttcgg cgacctggtg gcgataactc
7441 aaagaggtgg tgtcctcaat ggccagcagt tcgggaaact cctgagccaa cttgactgtt
7501 tgcatggcgc cagcctttct gatcgcctcg gcagaaacgt tgggattgcg gtaaaatcgg
7561 taagcgcctt cctgcatggc ttcactaccc tctgatgtga tggggattga tttaccagaa
7621 tattttgcca attgggcggc gacgttaacc aagcgggcag tacggcgtgg atcacccagc
7681 gccgccgaag agaacacaga tttagcccag tcggccgcac gatgaagagc agaagttatc
7741 atgaacgtta ccatgttagg aggtcacatg gaatatcaga ttctggaaaa cgggaaaggt
7801 tccgttcagg acgctacttg tgtataagag tcaggtgagt tatgttgttg ctttactttt
7861 tagcttgctt gctggaacta cattaggtat ttggggtatt gctattgtta caggaattca
7921 tcgatatcta gatctcgagc tcgcgaaagc ttgcatgcct gcaggtcccc gggatcgtct
7981 tgccttgctc gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc ttgatggagc
8041 gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt
8101 catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc
8161 tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg
8221 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg
8281 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg
8341 gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt
8401 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt
8461 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
8521 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
8581 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
8641 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc
8701 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaagatcg tcgacctgca
8761 ggcatgcaag ctttcgcgag ctcgagatct agaatcgtcc attccgacag catcgccagt
8821 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
8881 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagc
//
