pdCas9-bacteria

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pdCas9-bacteria 
出品公司: 爱迪基因
目录编号: 44249
存储实验室: Stanley Qi 

载体骨架基本信息
载体名称: p15A
载体抗性: 氯霉素
完整质粒大小: 6705 bp
载体类型: 细菌表达,CRISPR
高拷贝/低拷贝: 低拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: dCas9 (细菌)
物种来源: S.化脓性链球菌
插入大小: 4107 bp
基因突变: D10A H840A (催化活性)
启动子 pLtetO-1
克隆方法: 限制性内切酶
5' 克隆位点: BglII(未破坏)
3' 克隆位点: XhoI (未破坏)
5' 测序引物 ttcaaaaGATCTAAAGAGGAGAAAGGATCTATGGATAAGAAATACTCAATAG
3' 测序引物 tgcctggagatccttactcgaGTTAGTCACCTCCTAGCTGACTCAAATCAAT

文献引用方法
材料和方法部分: pdCas9-bacteria was a gift from Stanley Qi (Addgene plasmid # 44249)
参考文献部分:
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860

载体图谱质粒图谱和多克隆位点信息

pdCas9-bacteria载体图谱

载体简介

载体序列

LOCUS       pdCas9-bacteria	6705 bp 	DNA	SYN
DEFINITION  pdCas9-bacteria
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="pdCas9-bacteria"
                     /mol_type="other DNA"
     CDS             complement(7..633)
                     /label="ORF frame 1"
     misc_feature    complement(21..627)
                     /label="TetR"
     CDS             740..4846
                     /label="ORF frame 2"
     terminator      4873..4916
                     /label="rrnB_T1_terminator"
     gene            complement(5920..6579)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             complement(5920..6579)
                     /label="ORF frame 1"
ORIGIN
    1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
   61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
  121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
  181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
  241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
  301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
  361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
  421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
  481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
  541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
  601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
  661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaaGATC
  721 TAAAGAGGAG AAAGGATCTA TGGATAAGAA ATACTCAATA GGCTTAGCTA TCGGCACAAA
  781 TAGCGTCGGA TGGGCGGTGA TCACTGATGA ATATAAGGTT CCGTCTAAAA AGTTCAAGGT
  841 TCTGGGAAAT ACAGACCGCC ACAGTATCAA AAAAAATCTT ATAGGGGCTC TTTTATTTGA
  901 CAGTGGAGAG ACAGCGGAAG CGACTCGTCT CAAACGGACA GCTCGTAGAA GGTATACACG
  961 TCGGAAGAAT CGTATTTGTT ATCTACAGGA GATTTTTTCA AATGAGATGG CGAAAGTAGA
 1021 TGATAGTTTC TTTCATCGAC TTGAAGAGTC TTTTTTGGTG GAAGAAGACA AGAAGCATGA
 1081 ACGTCATCCT ATTTTTGGAA ATATAGTAGA TGAAGTTGCT TATCATGAGA AATATCCAAC
 1141 TATCTATCAT CTGCGAAAAA AATTGGTAGA TTCTACTGAT AAAGCGGATT TGCGCTTAAT
 1201 CTATTTGGCC TTAGCGCATA TGATTAAGTT TCGTGGTCAT TTTTTGATTG AGGGAGATTT
 1261 AAATCCTGAT AATAGTGATG TGGACAAACT ATTTATCCAG TTGGTACAAA CCTACAATCA
 1321 ATTATTTGAA GAAAACCCTA TTAACGCAAG TGGAGTAGAT GCTAAAGCGA TTCTTTCTGC
 1381 ACGATTGAGT AAATCAAGAC GATTAGAAAA TCTCATTGCT CAGCTCCCCG GTGAGAAGAA
 1441 AAATGGCTTA TTTGGGAATC TCATTGCTTT GTCATTGGGT TTGACCCCTA ATTTTAAATC
 1501 AAATTTTGAT TTGGCAGAAG ATGCTAAATT ACAGCTTTCA AAAGATACTT ACGATGATGA
 1561 TTTAGATAAT TTATTGGCGC AAATTGGAGA TCAATATGCT GATTTGTTTT TGGCAGCTAA
 1621 GAATTTATCA GATGCTATTT TACTTTCAGA TATCCTAAGA GTAAATACTG AAATAACTAA
 1681 GGCTCCCCTA TCAGCTTCAA TGATTAAACG CTACGATGAA CATCATCAAG ACTTGACTCT
 1741 TTTAAAAGCT TTAGTTCGAC AACAACTTCC AGAAAAGTAT AAAGAAATCT TTTTTGATCA
 1801 ATCAAAAAAC GGATATGCAG GTTATATTGA TGGGGGAGCT AGCCAAGAAG AATTTTATAA
 1861 ATTTATCAAA CCAATTTTAG AAAAAATGGA TGGTACTGAG GAATTATTGG TGAAACTAAA
 1921 TCGTGAAGAT TTGCTGCGCA AGCAACGGAC CTTTGACAAC GGCTCTATTC CCCATCAAAT
 1981 TCACTTGGGT GAGCTGCATG CTATTTTGAG AAGACAAGAA GACTTTTATC CATTTTTAAA
 2041 AGACAATCGT GAGAAGATTG AAAAAATCTT GACTTTTCGA ATTCCTTATT ATGTTGGTCC
 2101 ATTGGCGCGT GGCAATAGTC GTTTTGCATG GATGACTCGG AAGTCTGAAG AAACAATTAC
 2161 CCCATGGAAT TTTGAAGAAG TTGTCGATAA AGGTGCTTCA GCTCAATCAT TTATTGAACG
 2221 CATGACAAAC TTTGATAAAA ATCTTCCAAA TGAAAAAGTA CTACCAAAAC ATAGTTTGCT
 2281 TTATGAGTAT TTTACGGTTT ATAACGAATT GACAAAGGTC AAATATGTTA CTGAAGGAAT
 2341 GCGAAAACCA GCATTTCTTT CAGGTGAACA GAAGAAAGCC ATTGTTGATT TACTCTTCAA
 2401 AACAAATCGA AAAGTAACCG TTAAGCAATT AAAAGAAGAT TATTTCAAAA AAATAGAATG
 2461 TTTTGATAGT GTTGAAATTT CAGGAGTTGA AGATAGATTT AATGCTTCAT TAGGTACCTA
 2521 CCATGATTTG CTAAAAATTA TTAAAGATAA AGATTTTTTG GATAATGAAG AAAATGAAGA
 2581 TATCTTAGAG GATATTGTTT TAACATTGAC CTTATTTGAA GATAGGGAGA TGATTGAGGA
 2641 AAGACTTAAA ACATATGCTC ACCTCTTTGA TGATAAGGTG ATGAAACAGC TTAAACGTCG
 2701 CCGTTATACT GGTTGGGGAC GTTTGTCTCG AAAATTGATT AATGGTATTA GGGATAAGCA
 2761 ATCTGGCAAA ACAATATTAG ATTTTTTGAA ATCAGATGGT TTTGCCAATC GCAATTTTAT
 2821 GCAGCTGATC CATGATGATA GTTTGACATT TAAAGAAGAC ATTCAAAAAG CACAAGTGTC
 2881 TGGACAAGGC GATAGTTTAC ATGAACATAT TGCAAATTTA GCTGGTAGCC CTGCTATTAA
 2941 AAAAGGTATT TTACAGACTG TAAAAGTTGT TGATGAATTG GTCAAAGTAA TGGGGCGGCA
 3001 TAAGCCAGAA AATATCGTTA TTGAAATGGC ACGTGAAAAT CAGACAACTC AAAAGGGCCA
 3061 GAAAAATTCG CGAGAGCGTA TGAAACGAAT CGAAGAAGGT ATCAAAGAAT TAGGAAGTCA
 3121 GATTCTTAAA GAGCATCCTG TTGAAAATAC TCAATTGCAA AATGAAAAGC TCTATCTCTA
 3181 TTATCTCCAA AATGGAAGAG ACATGTATGT GGACCAAGAA TTAGATATTA ATCGTTTAAG
 3241 TGATTATGAT GTCGATGCCA TTGTTCCACA AAGTTTCCTT AAAGACGATT CAATAGACAA
 3301 TAAGGTCTTA ACGCGTTCTG ATAAAAATCG TGGTAAATCG GATAACGTTC CAAGTGAAGA
 3361 AGTAGTCAAA AAGATGAAAA ACTATTGGAG ACAACTTCTA AACGCCAAGT TAATCACTCA
 3421 ACGTAAGTTT GATAATTTAA CGAAAGCTGA ACGTGGAGGT TTGAGTGAAC TTGATAAAGC
 3481 TGGTTTTATC AAACGCCAAT TGGTTGAAAC TCGCCAAATC ACTAAGCATG TGGCACAAAT
 3541 TTTGGATAGT CGCATGAATA CTAAATACGA TGAAAATGAT AAACTTATTC GAGAGGTTAA
 3601 AGTGATTACC TTAAAATCTA AATTAGTTTC TGACTTCCGA AAAGATTTCC AATTCTATAA
 3661 AGTACGTGAG ATTAACAATT ACCATCATGC CCATGATGCG TATCTAAATG CCGTCGTTGG
 3721 AACTGCTTTG ATTAAGAAAT ATCCAAAACT TGAATCGGAG TTTGTCTATG GTGATTATAA
 3781 AGTTTATGAT GTTCGTAAAA TGATTGCTAA GTCTGAGCAA GAAATAGGCA AAGCAACCGC
 3841 AAAATATTTC TTTTACTCTA ATATCATGAA CTTCTTCAAA ACAGAAATTA CACTTGCAAA
 3901 TGGAGAGATT CGCAAACGCC CTCTAATCGA AACTAATGGG GAAACTGGAG AAATTGTCTG
 3961 GGATAAAGGG CGAGATTTTG CCACAGTGCG CAAAGTATTG TCCATGCCCC AAGTCAATAT
 4021 TGTCAAGAAA ACAGAAGTAC AGACAGGCGG ATTCTCCAAG GAGTCAATTT TACCAAAAAG
 4081 AAATTCGGAC AAGCTTATTG CTCGTAAAAA AGACTGGGAT CCAAAAAAAT ATGGTGGTTT
 4141 TGATAGTCCA ACGGTAGCTT ATTCAGTCCT AGTGGTTGCT AAGGTGGAAA AAGGGAAATC
 4201 GAAGAAGTTA AAATCCGTTA AAGAGTTACT AGGGATCACA ATTATGGAAA GAAGTTCCTT
 4261 TGAAAAAAAT CCGATTGACT TTTTAGAAGC TAAAGGATAT AAGGAAGTTA AAAAAGACTT
 4321 AATCATTAAA CTACCTAAAT ATAGTCTTTT TGAGTTAGAA AACGGTCGTA AACGGATGCT
 4381 GGCTAGTGCC GGAGAATTAC AAAAAGGAAA TGAGCTGGCT CTGCCAAGCA AATATGTGAA
 4441 TTTTTTATAT TTAGCTAGTC ATTATGAAAA GTTGAAGGGT AGTCCAGAAG ATAACGAACA
 4501 AAAACAATTG TTTGTGGAGC AGCATAAGCA TTATTTAGAT GAGATTATTG AGCAAATCAG
 4561 TGAATTTTCT AAGCGTGTTA TTTTAGCAGA TGCCAATTTA GATAAAGTTC TTAGTGCATA
 4621 TAACAAACAT AGAGACAAAC CAATACGTGA ACAAGCAGAA AATATTATTC ATTTATTTAC
 4681 GTTGACGAAT CTTGGAGCTC CCGCTGCTTT TAAATATTTT GATACAACAA TTGATCGTAA
 4741 ACGATATACG TCTACAAAAG AAGTTTTAGA TGCCACTCTT ATCCATCAAT CCATCACTGG
 4801 TCTTTATGAA ACACGCATTG ATTTGAGTCA GCTAGGAGGT GACTAACtcg agtaaggatc
 4861 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
 4921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
 4981 tgcgtttata cctagggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg
 5041 ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca
 5101 ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg
 5161 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
 5221 actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc
 5281 ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga
 5341 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
 5401 agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg
 5461 caaaagcacc actggcagca gccactggta attgatttag aggagttagt cttgaagtca
 5521 tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag
 5581 ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg
 5641 gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat
 5701 cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca
 5761 cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc accaataaaa
 5821 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
 5881 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
 5941 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
 6001 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
 6061 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
 6121 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
 6181 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
 6241 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
 6301 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
 6361 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
 6421 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
 6481 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
 6541 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
 6601 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
 6661 ggaacctctt acgtgccgat caacgtctca ttttcgccag atatc
//

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