| 出品公司: | Molecularcloud |
|---|---|
| 载体名称: | pcrF19NM2 |
| 载体又名: | pcrF19NM2载体，pcrF19NM2质粒，pcrF19NM2载体质粒 |
| 英文名称: | pcrF19NM2, pcrF19NM2 Vector, pcrF19NM2 Plasmid |
| 质粒类型: | 枯草芽孢杆菌载体，Crispr载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 4.1 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | mCherry |
| 克隆菌株: | JM109，DH5α,TOP10 等 |
| 宿主细胞: | 枯草芽孢杆菌，Bacillus subtilis |
| 备注: | 该质粒用于在枯草芽孢杆菌中表达crRNA array（复制起点具有温度敏感性），并在大肠杆菌中能够复制。质粒中的mCherry可通过同源替换用于快速筛选。 |
| 产品目录号: | MC_0101256 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pcrF19NM2载体质粒基本信息
pcrF19NM2质粒图谱载体图谱和pcrF19NM2载体序列质粒序列多克隆位点信息
pcrF19NM2质粒载体简介
pcrF19NM2质粒序列载体序列
LOCUS pCrF19NM2 4063 bp DNA circular SYN 03-MAR-2026
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4063)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 3, 2026 from SnapGene 8.1.0 to Vector NTI(R) format
https://www.biofeng.com
COMMENT Sequence Label: pCrF19NM2
COMMENT VNTDATE|1122768000|
COMMENT VNTDBDATE|1122768000|
COMMENT VNTNAME|pCrF19NM2|
FEATURES Location/Qualifiers
rep_origin 111..699
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 3242..3951
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
ORIGIN
1 atgtcatgac attggtgtac agaatggcga atggccatga ccaaaatccc ttaacgtgag
61 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
781 gattctgtgg ataaccgtat taccgccttt gagtgagctg aaattatgag gggatctctc
841 agagctcgag gtcatcgttc aaaatggtat gcgttttgac acatccacta tatatccgtg
901 tcgttctgtc cactcctgaa tcccattcca gaaattctct agcgattcca gaagtttctc
961 agagtcggaa agttgaccag acattacgaa ctggcacaga tggtcataac ctgaaggaag
1021 atctgattgc ttaactgctt cagttaagac cgaagcgctc gtcgtataac agatgcgatg
1081 atgcagacca atcaacatgg cacctgccat tgctacctgc acagtcaagg atggtagaaa
1141 tgttgtcggt ccttgcacac gaatattacg ccatttgcct gcatattcaa acagctcttc
1201 tacgataagg gcacaaatcg catcgtggaa cgtttgggct tctaccgatt tagcagtttg
1261 atacactttc tctaagtatc cacctgaatc ataaatcggc aaaatagaga aaaattgacc
1321 atgtgtaagc ggccaatctg attccacctg agatgcataa tctagtagaa tctcttcgct
1381 atcaaaattc acttccacct tccactcacc ggttgtccat tcatggctga actctgcttc
1441 ctctgttgac atgacacaca tcatctcaat atccgaatag ggcccatcag tctgacgacc
1501 aagagagcca taaacaccaa tagccttaac atcatcccca tatttatcca atattcgttc
1561 cttaatttca tgaacaatct tcattctttc ttctctagtc attattattg gtccattcac
1621 tattctcatt cccctttcag ataattttag atttgctttt ctaaataaga atatttggag
1681 agcaccgttc ttattcagct attaaaccca ttatatcggg tttttgaggg gatttcaact
1741 gcagacacct aaattcaaaa tctatcggtc agatttatac cgatttgatt ttatatattc
1801 ttgaataaca tacgccgagt tatcacataa aagcgggaac caatcatcaa atttaaactt
1861 cattgcataa tccattaaac tcttaaattc tacgattcct tgttcatcaa taaactcaat
1921 catttcttta attaatttat atctatctgt tgttgttttc tttaataatt catcaacatc
1981 tacaccgcca taaactatca tatcttcttt ttgatattta aatttattag gatcgtccat
2041 gtgaagcata tatctcacaa gacctttcac acttcctgca atctgcggaa tagtcgcatt
2101 caattcttct gtaattattt ttatctgttc ataagattta ttaccctcat acatcactag
2161 aatatgataa tgctcttttt tcatcctatc ttctgtatca gtatccctat catgtaatgg
2221 agacactaca aattgaatgt gtaactcttt taaatactct aaccactcgg cttttgctga
2281 ttctggatat aaaacaaatg tccaattacg tcctcttgaa tttttcttgt tttcagtttc
2341 ttttattaca ttttcgctca tgatataata acggtgctaa tacatttaac aaaatttagt
2401 catagatagg cagcatgcca gtgctgtcta tctttttttg tttaaaatgc accgtattcc
2461 tcctttgcat atttttttat tagaataccg gttgcatctg atttgctaat attatatttt
2521 tctttgattc tatttaatat ctcattttct tctgttgtaa gtcttaaagt aacagcaact
2581 tttttctctt cttttctatc tacaaccatc actgtacctc ccaacatctg tttttttcac
2641 tttaacataa aaaacaacct tttaacatta aaaacccaat atttatttat ttgtttggac
2701 aatggacaat ggacacctag gggggaggtc gtagtacccc cctatgtttt ctcccctaaa
2761 taaccccaaa aatctaagaa aaaaagacct caaaaaggtc tttaattaac atctcaaatt
2821 tcgcatttat tccaatttcc tttttgcgtg tgatgcgtta ttaacgttga tataatttaa
2881 attttatttg acaaaaatgg gctcgtgttg tacaataaat gtagaggtag agacgcgagg
2941 tctaagaact ttaaataatt tctactgttg tagatagaga ccgtgaagtt aataaggtct
3001 caaatttcta ctgttgtaga tcgtctctga actgattcaa gcaagcttaa acccagctca
3061 atgagctggg ttttttgttt gttttttcaa acttagttag cttggccagt gcctctagag
3121 tcttaaatac caaaaatgtg ttgacaacca gcagataaaa tgctataatg ctagagtgat
3181 agcggtaccc tcgagaaaag gaggaaaaaa aatgtttatg aaatctactg gtattgtacg
3241 tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
3301 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
3361 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
3421 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
3481 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
3541 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
3601 cggcgaattc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
3661 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
3721 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
3781 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
3841 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
3901 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagagtcga
3961 cctgttacga acggcagatc agaattttgt aataaaaaaa gagcctgctc attacactgc
4021 gggctctttt tcatggtcag aagacgggta accaagataa caa
//
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pcrF19NM2质粒载体应用举例
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