| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001679 |
| 产品用途: | 3' AAVLINK plasmid for ABCC6 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ABCC6 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABCC6-FLAG 7095 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7095)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7095
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3808
/codon_start=1
/label=ABCC6
/translation="GMNLSGGQKQRLSLARAVYRKAAVYLLDDPLAALDAHVGQHVFNQ
VIGPGGLLQGTTRILVTHALHILPQADWIIVLANGAIAEMGSYQELLQRKGALMCLLDQ
ARQPGDRGEGETEPGTSTKDPRGTSAGRRPELRRERSIKSVPEKDRTTSEAQTEVPLDD
PDRAGWPAGKDSIQYGRVKATVHLAYLRAVGTPLCLYALFLFLCQQVASFCRGYWLSLW
ADDPAVGGQQTQAALRGGIFGLLGCLQAIGLFASMAAVLLGGARASRLLFQRLLWDVVR
SPISFFERTPIGHLLNRFSKETDTVDVDIPDKLRSLLMYAFGLLEVSLVVAVATPLATV
AILPLFLLYAGFQSLYVVSSCQLRRLESASYSSVCSHMAETFQGSTVVRAFRTQAPFVA
QNNARVDESQRISFPRLVADRWLAANVELLGNGLVFAAATCAVLSKAHLSAGLVGFSVS
AALQVTQTLQWVVRNWTDLENSIVSVERMQDYAWTPKEAPWRLPTCAAQPPWPQGGQIE
FRDFGLRYRPELPLAVQGVSFKIHAGEKVGIVGRTGAGKSSLASGLLRLQEAAEGGIWI
DGVPIAHVGLHTLRSRISIIPQDPILFPGSLRMNLDLLQEHSDEAIWAALETVQLKALV
ASLPGQLQYKCADRGEDLSVGQKQLLCLARALLRKTQILILDEATAAVDPGTELQMQAM
LGSWFAQCTVLLIAHRLRSVMDCARVLVMDKGQVAESGSPAQLLAQKGLFYRLAQESGL
V"
misc_feature 1940..1945
/label=SDS
misc_feature 2178..2183
/label=SDS
misc_feature 3030..3035
/label=SDS
misc_feature 3246..3251
/label=SDS
CDS 3815..3880
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3884..4315
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4028..4033
/label=SDS
repeat_region 4358..4498
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4358..4487
/label=AAV2 ITR
rep_origin 4573..5028
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5310..5414
/gene="bla"
/label=AmpR promoter
CDS 5415..6275
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6446..7034
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca tgaatctctc
1561 cggaggccag aagcagcggc tgagcctggc ccgggctgta tacagaaagg cagctgtgta
1621 cctgctggat gaccccctgg cggccctgga tgcccacgtt ggccagcatg tcttcaacca
1681 ggtcattggg cctggtgggc tactccaggg aacaacacgg attctcgtga cgcacgcact
1741 ccacatcctg ccccaggctg attggatcat agtgctggca aatggggcca tcgcagagat
1801 gggttcctac caggagcttc tgcagaggaa gggggccctc atgtgtcttc tggatcaagc
1861 cagacagcca ggagatagag gagaaggaga aacagaacct gggaccagca ccaaggaccc
1921 cagaggcacc tctgcaggca ggaggcccga gcttagacgc gagaggtcca tcaagtcagt
1981 ccctgagaag gaccgtacca cttcagaagc ccagacagag gttcctctgg atgaccctga
2041 cagggcagga tggccagcag gaaaggacag catccaatac ggcagggtga aggccacagt
2101 gcacctggcc tacctgcgtg ccgtgggcac ccccctctgc ctctacgcac tcttcctctt
2161 cctctgccag caagtggcct ccttctgccg gggctactgg ctgagcctgt gggcggacga
2221 ccctgcagta ggtgggcagc agacgcaggc agccctgcgt ggcgggatct tcgggctcct
2281 cggctgtctc caagccattg ggctgtttgc ctccatggct gcggtgctcc taggtggggc
2341 ccgggcatcc aggttgctct tccagaggct cctgtgggat gtggtgcgat ctcccatcag
2401 cttctttgag cggacaccca ttggtcacct gctaaaccgc ttctccaagg agacagacac
2461 ggttgacgtg gacattccag acaaactccg gtccctgctg atgtacgcct ttggactcct
2521 ggaggtcagc ctggtggtgg cagtggctac cccactggcc actgtggcca tcctgccact
2581 gtttctcctc tacgctgggt ttcagagcct gtatgtggtt agctcatgcc agctgagacg
2641 cttggagtca gccagctact cgtctgtctg ctcccacatg gctgagacgt tccagggcag
2701 cacagtggtc cgggcattcc gaacccaggc cccctttgtg gctcagaaca atgctcgcgt
2761 agatgaaagc cagaggatca gtttcccgcg actggtggct gacaggtggc ttgcggccaa
2821 tgtggagctc ctggggaatg gcctggtgtt tgcagctgcc acgtgtgctg tgctgagcaa
2881 agcccacctc agtgctggcc tcgtgggctt ctctgtctct gctgccctcc aggtgaccca
2941 gacactgcag tgggttgttc gcaactggac agacctagag aacagcatcg tgtcagtgga
3001 gcggatgcag gactatgcct ggacgcccaa ggaggctccc tggaggctgc ccacatgtgc
3061 agctcagccc ccctggcctc agggcgggca gatcgagttc cgggactttg ggctaagata
3121 ccgacctgag ctcccgctgg ctgtgcaggg cgtgtccttc aagatccacg caggagagaa
3181 ggtgggcatc gttggcagga ccggggcagg gaagtcctcc ctggccagtg ggctgctgcg
3241 gctccaggag gcagctgagg gtgggatctg gatcgacggg gtccccattg cccacgtggg
3301 gctgcacaca ctgcgctcca ggatcagcat catcccccag gaccccatcc tgttccctgg
3361 ctctctgcgg atgaacctcg acctgctgca ggagcactcg gacgaggcta tctgggcagc
3421 cctggagacg gtgcagctca aagccttggt ggccagcctg cccggccagc tgcagtacaa
3481 gtgtgctgac cgaggcgagg acctgagcgt gggccagaaa cagctcctgt gtctggcacg
3541 tgcccttctc cggaagaccc agatcctcat cctggacgag gctactgctg ccgtggaccc
3601 tggcacggag ctgcagatgc aggccatgct cgggagctgg tttgcacagt gcactgtgct
3661 gctcattgcc caccgcctgc gctccgtgat ggactgtgcc cgggttctgg tcatggacaa
3721 ggggcaggtg gcagagagcg gcagcccggc ccagctgctg gcccagaagg gcctgtttta
3781 cagactggcc caggagtcag gcctggtctg tacagactac aaagaccatg acggtgatta
3841 taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga taatcaacct
3901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
3961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
4021 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
4081 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt
4141 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat
4201 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
4261 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc
4321 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
4381 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
4441 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
4501 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
4561 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
4621 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
4681 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
4741 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
4801 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
4861 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
4921 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
4981 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
5041 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
5101 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
5161 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
5221 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
5281 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
5341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
5401 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
5461 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
5521 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
5581 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
5641 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
5701 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
5761 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
5821 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
5881 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
5941 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
6001 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
6061 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
6121 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
6181 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
6241 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
6301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
6361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
7081 cttttgctca catgt
//
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