| 出品公司: | Clontech |
|---|---|
| 载体名称: | pBI101-T-DNA |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 5350 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | -- |
| 载体标签: | GUS |
| 载体抗性: | 无 |
| 筛选标记: | 无 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | 无抗性标记，在大肠杆菌中用蓝白斑法筛选 |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
LOCUS pBI101 T-DNA Region 5350 bp DNA SYN
DEFINITION pBI101 T-DNA Region
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5350
/organism="pBI101"
/mol_type="other DNA"
CDS 386..1180
/label="ORF frame 2"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 389..1177
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(695..1231)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
promoter 2407..2436
/label="lac_promoter"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 2450..2472
/label="M13_pUC_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
promoter 2471..2489
/label="M13_reverse_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS 2552..4360
/label="ORF frame 2"
/translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA
HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ*"
CDS complement(2666..3127)
/label="ORF frame 1"
/translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
misc_feature 4689..4832
/label="lacZ_a"
/translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
promoter complement(4692..4708)
/label="M13_forward20_primer"
/translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
misc_feature complement(4701..4723)
/label="M13_pUC_fwd_primer"
/translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
rep_origin 4850..5084
/label="f1_origin"
/translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
ORIGIN
1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
121 acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
181 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
241 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
301 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
361 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
1021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
1081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
1141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
1201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
1261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
1321 ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
1381 ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
1801 tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
1861 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
1921 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
1981 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
2041 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
2101 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
2161 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
2221 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
2341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
2401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
2461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
2521 ctctagagga tccccgggtg gtcagtccct tatgttacgt cctgtagaaa ccccaacccg
2581 tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
2641 tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag
2701 ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca
2761 gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
2821 ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
2881 ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
2941 caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
3001 cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccggaat
3061 ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
3121 gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
3181 tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
3241 tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
3301 tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg
3361 catccggtca gtggcagtga agggccaaca gttcctgatt aaccacaaac cgttctactt
3421 tactggcttt ggtcgtcatg aagatgcgga cttacgtggc aaaggattcg ataacgtgct
3481 gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca
3541 ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
3601 tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
3661 gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca
3721 ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
3781 tgccaacgaa ccggataccc gtccgcaagt gcacgggaat atttcgccac tggcggaagc
3841 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
3901 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
3961 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
4021 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
4081 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
4141 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
4201 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
4261 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
4321 gaacttcggt gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc
4381 accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc gatcgttcaa
4441 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
4501 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
4561 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
4621 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
4681 atcgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
4741 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
4801 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt
4861 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4921 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
4981 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
5041 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
5101 gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat
5161 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
5221 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat
5281 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
5341 tatcctgcca
//
