pBI101 T-DNA

价格:1800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pBI101-T-DNA
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 5350 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: --
载体标签: GUS
载体抗性:
筛选标记:
克隆菌株: --
宿主细胞(系): 植物细胞、农杆菌
备注: 无抗性标记,在大肠杆菌中用蓝白斑法筛选
产品目录号: --
稳定性: 瞬表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pBI101-T-DNA载体图谱



pBI101-T-DNA 多克隆位点

载体简介

载体序列


LOCUS       pBI101 T-DNA Region	5350 bp 	DNA	SYN
DEFINITION  pBI101 T-DNA Region
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="pBI101"
                     /mol_type="other DNA"
     CDS             386..1180
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            389..1177
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(695..1231)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     promoter        2407..2436
                     /label="lac_promoter"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    2450..2472
                     /label="M13_pUC_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     promoter        2471..2489
                     /label="M13_reverse_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             2552..4360
                     /label="ORF frame 2"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDA
                     HTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNK
                     KGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ*"
     CDS             complement(2666..3127)
                     /label="ORF frame 1"
                     /translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
                     HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
                     RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
     misc_feature    4689..4832
                     /label="lacZ_a"
                     /translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
                     HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
                     RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
     promoter        complement(4692..4708)
                     /label="M13_forward20_primer"
                     /translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
                     HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
                     RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
     misc_feature    complement(4701..4723)
                     /label="M13_pUC_fwd_primer"
                     /translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
                     HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
                     RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
     rep_origin      4850..5084
                     /label="f1_origin"
                     /translation="MRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGN
                     HHSRRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSD
                     RIETQHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*"
ORIGIN
    1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
   61 aatctgatca tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
  121 acaagccgtt ttacgtttgg aactgacaga accgcaacga ttgaaggagc cactcagccg
  181 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
  241 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
  301 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
  361 ctgcaccgga tctggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
  421 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
  481 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
  541 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
  601 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
  661 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
  721 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
  781 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
  841 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
  901 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
  961 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
 1021 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
 1081 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
 1141 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
 1201 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
 1261 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
 1321 ggggatctca tgctggagtt cttcgcccag ggatctctgc ggaacaggcg gtcgaaggtg
 1381 ccgatatcat tacgacagca acggccgacg agcacaacgc cacgatcctg agcgacaata
 1441 tgatcgggcc cggcgtccac atcaacggcg tcggcggcga ctgcccaggc aagaccgaga
 1501 tgcaccgcga tatcttgctg cgttcggata ttttcgtgga gttcccgcca cagacccgga
 1561 tgatccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
 1621 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
 1681 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
 1741 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
 1801 tgtcatctat gttactagat cggggcctcc tgtcaatgct ggcggcggct ctggtggtgg
 1861 ttctggtggc ggctctgagg gtggtggctc tgagggtggc ggttctgagg gtggcggctc
 1921 tgagggaggc ggttccggtg gtggctctgg ttccggtgat tttgattatg aaaagatggc
 1981 aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa aacgcgctac agtctgacgc
 2041 taaaggcaaa cttgattctg tcgctactga ttacggtgct gctatcgatg gtttcattgg
 2101 tgacgtttcc ggccttgcta atggtaatgg tgctactggt gattttgctg gctctaattc
 2161 ccaaatggct caagtcggtg acggtgataa ttcaccttta atgaataatt tccgtcaata
 2221 tttaccttcc ctccctcaat cggttgaatg tcgccctttt gtctttggcc caatacgcaa
 2281 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
 2341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
 2401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
 2461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga
 2521 ctctagagga tccccgggtg gtcagtccct tatgttacgt cctgtagaaa ccccaacccg
 2581 tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
 2641 tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag
 2701 ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca
 2761 gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
 2821 ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
 2881 ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
 2941 caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
 3001 cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccggaat
 3061 ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
 3121 gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
 3181 tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
 3241 tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
 3301 tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg
 3361 catccggtca gtggcagtga agggccaaca gttcctgatt aaccacaaac cgttctactt
 3421 tactggcttt ggtcgtcatg aagatgcgga cttacgtggc aaaggattcg ataacgtgct
 3481 gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca
 3541 ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
 3601 tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
 3661 gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca
 3721 ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
 3781 tgccaacgaa ccggataccc gtccgcaagt gcacgggaat atttcgccac tggcggaagc
 3841 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
 3901 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
 3961 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
 4021 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
 4081 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
 4141 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
 4201 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
 4261 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
 4321 gaacttcggt gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc
 4381 accatcgtcg gctacagcct cgggaattgc taccgagctc gaatttcccc gatcgttcaa
 4441 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
 4501 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
 4561 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
 4621 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
 4681 atcgggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
 4741 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
 4801 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgcccgctc ctttcgcttt
 4861 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
 4921 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
 4981 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
 5041 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
 5101 gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat
 5161 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
 5221 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cccagtacat
 5281 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
 5341 tatcctgcca
//

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