| 质粒名称: | pLPCX2-MT1-MMP-eGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 89819 |
| 存储实验室: | Shengyu Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pLNCX2 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6100 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 逆转录病毒表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MT1-MMP,MMP14 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | NM_004995.3 |
| 启动子 | -- |
| 标签/融合蛋白: | eGFP（插入件上的C端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLPCX2-MT1-MMP-eGFP was a gift from Shengyu Yang (Addgene plasmid # 89819 ; http://n2t.net/addgene:89819 ; RRID:Addgene_89819) |
| 参考文献部分: | STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates invadopodium formation and melanoma invasion. Sun J, Lu F, He H, Shen J, Messina J, Mathew R, Wang D, Sarnaik AA, Chang WC, Kim M, Cheng H, Yang S. J Cell Biol. 2014 Nov 24;207(4):535-48. doi: 10.1083/jcb.201407082. Epub 2014 Nov 17. 10.1083/jcb.201407082 PubMed 25404747 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLPCX2-MT1-MMP-eGFP 8743 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express in MT1-MMP mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pLPCX2-MT1-MMP-eGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8743)
AUTHORS Sun J, Lu F, He H, Shen J, Messina J, Mathew R, Wang D, Sarnaik AA,
Chang WC, Kim M, Cheng H, Yang S
TITLE STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates
invadopodium formation and melanoma invasion.
JOURNAL J Cell Biol. 2014 Nov 24;207(4):535-48. doi: 10.1083/jcb.201407082.
Epub 2014 Nov 17.
PUBMED 25404747
REFERENCE 2 (bases 1 to 8743)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8743
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS complement(1150..1161)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
regulatory 2353..2362
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2359..3078
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2359..3075
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2404..2425)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2665..2684)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3012..3033
/label=EGFP-C
/note="EGFP, forward primer"
LTR 3144..3737
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(3840..3862)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
protein_bind complement(3990..4023)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(4083..4116)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 4136..4155
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4236..4253)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4407..4995)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4487..4506)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5166..6026)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5789..5808
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6027..6131)
/gene="bla"
/label=AmpR promoter
primer_bind 6199..6217
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
LTR 6819..6994
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 7057..7256
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 7457..7873
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 7803..7825
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 7841..7857
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 7970..8569
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7970..7989)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8466..8486
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggactcag atctcgagct caagcttgga gtttctcgga ccatgtctcc cgccccaaga
601 cccccccgtt gtctcctgct ccccctgctc acgctcggca ccgcgctcgc ctccctcggc
661 tcggcccaaa gcagcagctt cagccccgaa gcctggctac agcaatatgg ctacctgcct
721 cccggggacc tacgtaccca cacacagcgc tcaccccagt cactctcagc ggccatcgct
781 gccatgcaga agttttacgg cttgcaagta acaggcaaag ctgatgcaga caccatgaag
841 gccatgaggc gcccccgatg tggtgttcca gacaagtttg gggctgagat caaggccaat
901 gttcgaagga agcgctacgc catccagggt ctcaaatggc aacataatga aatcactttc
961 tgcatccaga attacacccc caaggtgggc gagtatgcca catacgaggc cattcgcaag
1021 gcgttccgcg tgtgggagag tgccacacca ctgcgcttcc gcgaggtgcc ctatgcctac
1081 atccgtgagg gccatgagaa gcaggccgac atcatgatct tctttgccga gggcttccat
1141 ggcgacagca cgcccttcga tggtgagggc ggcttcctgg cccatgccta cttcccaggc
1201 cccaacattg gaggagacac ccactttgac tctgccgagc cttggactgt caggaatgag
1261 gatctgaatg gaaatgacat cttcctggtg gctgtgcacg agctgggcca tgccctgggg
1321 ctcgagcatt ccagtgaccc ctcggccatc atggcaccct tttaccagtg gatggacacg
1381 gagaattttg tgctgcccga tgatgaccgc cggggcatcc agcaacttta tgggggtgag
1441 tcagggttcc ccaccaagat gccccctcaa cccaggacta cctcccggcc ttctgttcct
1501 gataaaccca aaaaccccac ctatgggccc aacatctgtg acgggaactt tgacaccgtg
1561 gccatgctcc gaggggagat gtttgtcttc aaggagcgct ggttctggcg ggtgaggaat
1621 aaccaagtga tggatggata cccaatgccc attggccagt tctggcgggg cctgcctgcg
1681 tccatcaaca ctgcctacga gaggaaggat ggcaaattcg tcttcttcaa aggagacaag
1741 cattgggtgt ttgatgaggc gtccctggaa cctggctacc ccaagcacat taaggagctg
1801 ggccgagggc tgcctaccga caagattgat gctgctctct tctggatgcc caatggaaag
1861 acctacttct tccgtggaaa caagtactac cgtttcaacg aagagctcag ggcagtggat
1921 agcgagtacc ccaagaacat caaagtctgg gaagggatcc ctgagtctcc cagagggtca
1981 ttcatgggca gcgatgaagt cttcacttac ttctacaagg ggaacaaata ctggaaattc
2041 aacaaccaga agctgaaggt agaaccgggc taccccaagt cagccctgag ggactggatg
2101 ggctgcccat cgggaggccg gccggatgag gggactgagg aggagacgga ggtgatcatc
2161 attgaggtgg acgaggaggg cggcggggcg gtgagcgcgg ctgccgtggt gctgcccgtg
2221 ctgctgctgc tcctggtgct ggcggtgggc cttgcagtct tcttcttcag acgccatggg
2281 acccccaggc gactgctcta ctgccagcgt tccctgctgg acaaggtccc gcgggcccgg
2341 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
2401 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
2461 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
2521 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
2581 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
2641 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
2701 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
2761 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
2821 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
2881 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
2941 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
3001 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
3061 gacgagctgt acaagtaaag cggccgcctc ggccaaacat cgataaaata aaagatttta
3121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
3181 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
3241 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
3301 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
3361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
3421 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
3481 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
3541 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
3601 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
3661 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
3721 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac
3781 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
3841 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
3901 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
3961 cagtcacgta gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt
4021 tattaggtct gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta
4081 atataacttc gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca
4141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
4201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
4261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
4621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
4861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
4981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
5041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
5161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
5221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
5281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
5341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
5401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
5461 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
5521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
5581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
5641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
5701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
5761 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
5821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
5881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
5941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
6001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
6061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
6121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
6181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta
6241 gcaattgcta gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt
6301 gtccccctca cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc
6361 ccacactcac cggagccaaa gccgcggccc ttccgtttct ttgcttttga aagaccccac
6421 ccgtaggtgg caagctagct taagtaacgc cactttgcaa ggcatggaaa aatacataac
6481 tgagaataga aaagttcaga tcaaggtcag gaacaaagaa acagctgaat accaaacagg
6541 atatctgtgg taagcggttc ctgccccggc tcagggccaa gaacagatga gacagctgag
6601 tgatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcgg ggccaagaac
6661 agatggtccc cagatgcggt ccagccctca gcagtttcta gtgaatcatc agatgtttcc
6721 agggtgcccc aaggacctga aaatgaccct gtaccttatt tgaactaacc aatcagttcg
6781 cttctcgctt ctgttcgcgc gcttccgctc tccgagctca ataaaagagc ccacaacccc
6841 tcactcggcg cgccagtctt ccgatagact gcgtcgcccg ggtacccgta ttcccaataa
6901 agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg gtctcctctg
6961 agtgattgac tacccacgac gggggtcttt catttggggg ctcgtccggg atttggagac
7021 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg
7081 tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt actagttagc
7141 taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg
7201 caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag
7261 ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct
7321 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc
7381 ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
7441 tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct taggtcactg
7501 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
7561 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
7621 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
7681 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
7741 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
7801 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
7861 ttctctaggc gccggaatga tcccccgggc tgcaggtcgg ccgccacgac cggtgccgcc
7921 accatcccct gacccacgcc cctgacccct cacaaggaga cgaccttcca tgaccgagta
7981 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
8041 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
8101 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
8161 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
8221 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
8281 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
8341 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
8401 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
8461 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
8521 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
8581 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccaccc
8641 ggggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag taatagtaat
8701 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
