| 载体名称: | pXC18.4 |
|---|---|
| 载体又名: | pXC18.4载体，pXC18.4质粒，pXC18.4载体质粒 |
| 英文名称: | pXC18.4, pXC18.4 Vector, pXC18.4 Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4.1 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | GS高压筛选 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | 用于ChOK1SV等细胞的GS筛选哺乳细胞蛋白表达。 |
| 产品目录号: | 217530 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 相关载体: | pXC17.4 |
pXC18.4
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pXC18.4载体质粒基本信息
pXC18.4质粒图谱载体图谱和pXC18.4载体序列质粒序列多克隆位点信息
pXC18.4质粒载体简介
pXC18.4质粒序列载体序列
Snapgene载体序列下载: pXC18.4序列 .gb格式载体序列下载:pXC18.4载体序列
LOCUS pXC18.4 4059 bp DNA circular SYN 20-JUN-2026
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4059)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jun 20, 2026 from SnapGene 8.1.0
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4059
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 116..250
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(550..1138)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(join(1309..2100,2101..2169))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 1309 .. 2100 / #ccffcc
2: 2101 .. 2169 / #ccffcc / signal sequence
Cleavage site after base 2100"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2170..2274)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
terminator 2443..2470
/label=T7Te terminator
/note="phage T7 early transcription terminator"
/note="color: #ffffff; direction: RIGHT"
promoter 2547..3081
/label=mCMV
/note="color: #ffffff; direction: RIGHT"
intron 3175..4001
/label=CMV intron A
/note="human cytomegalovirus intron A (Chapman et al.,
1991)"
/note="color: #------"
ORIGIN
1 gaattcattg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
241 tgtctggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
301 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
361 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
421 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
481 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgacctc gggccgcgtt
541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
781 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
841 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
901 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
961 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
1021 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
1081 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
1141 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
1201 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
1261 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
1321 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
1381 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
1441 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
1501 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
1561 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
1621 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
1681 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
1741 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
1801 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
1861 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
1921 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
1981 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
2041 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
2101 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
2161 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
2221 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
2281 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
2341 aaataggcgt atcacgaggc cctgatggct ctttgcggca cccatcgttc gtaatgttcc
2401 gtggcaccga ggacaaccct caagagaaaa tgtaatcaca ctggctcacc ttcgggtggg
2461 cctttctgcg tttataagga gacactttat gtttaagaag gttggtaaat tccttgcggc
2521 tttggcagcc aagctagcgg ccgctgaggc gcgcctactg agtcattagg gactttccaa
2581 tgggttttgc ccagtacata aggtcaatag gggtgaatca acaggaaagt cccattggag
2641 ccaagtacac tgagtcaata gggactttcc attgggtttt gcccagtaca aaaggtcaat
2701 agggggtgag tcaatgggtt tttcccatta ttggcacgta cataaggtca ataggggtga
2761 gtcattgggt ttttccagcc aatttaatta aaacgccatg tactttccca ccattgacgt
2821 caatgggcta ttgaaactaa tgcaacgtga cctttaaacg gtactttccc atagctgatt
2881 aatgggaaag taccgttctc gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa
2941 acgtaacacc gccccggttt tcccctggaa attccatatt ggcacgcatt ctattggctg
3001 agctgcgttc tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg
3061 tctgaccacc gtagaacgca gcctcaggac ctccatagaa gacaccggga ccgatccagc
3121 ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt
3181 accgcctata gagtctatag gcccaccccc ttggcttctt atgcatgcta tactgttttt
3241 ggcttggggt ctatacaccc ccgcttcctc atgttatagg tgatggtata gcttagccta
3301 taggtgtggg ttattgacca ttattgacca ctcccctatt ggtgacgata ctttccatta
3361 ctaatccata acatggctct ttgccacaac tctctttatt ggctatatgc caatacactg
3421 tccttcagag actgacacgg actctgtatt tttacaggat ggggtctcat ttattattta
3481 caaattcaca tatacaacac caccgtcccc agtgcccgca gtttttatta aacataacgt
3541 gggatctcca cgcgaatctc gggtacgtgt tccggacatg ggctcttctc cggtagcggc
3601 ggagcttcta catccgagcc ctgctcccat gcctccagcg actcatggtc gctcggcagc
3661 tccttgctcc taacagtgga ggccagactt aggcacagca cgatgcccac caccaccagt
3721 gtgccgcaca aggccgtggc ggtagggtat gtgtctgaaa atgagctcgg ggagcgggct
3781 tgcaccgctg acgcatttgg aagacttaag gcagcggcag aagaagatgc aggcagctga
3841 gttgttgtgt tctgataaga gtcagaggta actcccgttg cggtgctgtt aacggtggag
3901 ggcagtgtag tctgagcagt actcgttgct gccgcgcgcg ccaccagaca taatagctga
3961 cagactaaca gactgttcct ttccatgggt cttttctgca gtcaccgtcc ttgacacgaa
4021 gcttcgcgac gtacgttcga acccgggcac gtgcggacc
//
