| 质粒名称: | pLYL76 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000595 |
| 产品用途: | Mammalian PhEF1α-driven constitutive MOR9-1 expression vector (PhEF1α-MOR9-1-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MOR9-1 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLYL76
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL76载体质粒基本信息
pLYL76质粒图谱载体图谱和pLYL76载体序列质粒序列多克隆位点信息
pLYL76质粒载体简介
pLYL76质粒序列载体序列
LOCUS pLYL76 6782 bp DNA circular SYN 13-JAN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6782)
AUTHORS iGEM CPU-CHINA 2020
TITLE Direct Submission
JOURNAL Exported Jul 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6782
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 173..1239
/label=P_EF1a
intron 292..1230
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
CDS 1268..2302
/codon_start=1
/product="Mus musculus olfactory receptor MOR9-1 gene,
complete cds
GenBank: AY073004.1"
/label=MOR9-1
/translation="MNGTEGPNFYVPFSNATGVVREFTRMSYSNHSSTSFFLTGLPGLE
TVYLWLSIPLCTMYIASLAGNGLILWVVKSEPSLHQPMYYFLSMLAVTDLGLSVSTLPT
MLTIYMMGVSEVALDMCLAQLFFIHTFSIMESSVLLTMAFDRVVAISSPLHYATILTNP
RVASLGMVILVRSIGLHIPAPIMLKKLPYCQKRHLSHSYCLHPDVMKLACTDTRINSAY
GLFVVLSTLGVDSVLIVLSYGLILYTVLSIASKTERLKALNTCVSHICSVLLFYTPMIG
LSMIHRFGKWASPCSRVLLSYLHFLTPPVLNPVVYTIKTKQIRQRIWCIFRCGGRSIGH
IQGH"
polyA_signal 2379..2603
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2649..3077
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 3091..3420
/label=P_SV40
/label=nonstandard type: promoter_const
misc_feature 3387..3392
/label=SDS
CDS 3487..4281
/label=Translation 2136-2930
misc_feature 3487..4278
/label=Kan/neoR
/label=nonstandard type: resistance
polyA_signal 4455..4576
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4621..4641)
/label=M13-rev
primer_bind complement(4625..4641)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4647..4669)
/label=LacO
protein_bind 4649..4665
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4673..4703)
/label=lac promoter
protein_bind 4718..4739
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 4845..5785
/label=ColE1
/note="ColE1"
CDS complement(5786..6646)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6647..6751)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggcta gcgggaaagt
181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
301 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
361 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
421 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
481 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
541 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
601 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
661 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
721 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
781 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
841 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
901 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
961 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
1021 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
1081 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
1141 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
1201 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacaagct
1261 tgccaccatg aatggcacag aaggccctaa cttctacgtg cccttctcca atgcgacggg
1321 tgtggtacgc gaattcacgc gtatgtccta ctccaatcat tccagcacct cattctttct
1381 tactggcctc cctggccttg agacagtgta tctctggctc tccattcctt tgtgcaccat
1441 gtacattgcc tctctggcag ggaatggctt gattctgtgg gttgtaaagt cagagccctc
1501 cctgcaccag cctatgtact actttctatc tatgcttgca gttactgacc tcggcctgtc
1561 tgtctccaca ctgcctacca tgctgacgat ctatatgatg ggtgtcagcg aagtggcatt
1621 agacatgtgc cttgcacagc tcttcttcat ccatactttc tccatcatgg agtcatctgt
1681 gctgctgact atggcctttg accgtgttgt ggccatcagc agtcccctac actatgccac
1741 catcctcacc aaccctcggg ttgccagttt gggcatggtc attttggtac gaagcattgg
1801 tctccacatc cctgccccca tcatgctgaa gaagctacct tattgccaga agcgtcatct
1861 ttcccactct tactgcctgc acccagatgt tatgaagctg gcctgtactg acacccgcat
1921 caacagtgcc tatggcctct ttgtggttct ctctactctg ggtgtggact ctgtgctcat
1981 cgttctatcc tatgggctga tcctctacac agtgctgtca atcgcttcca agactgaacg
2041 cctcaaagcc ctcaacacct gtgtttccca catctgttct gtgctgctct tctacacacc
2101 tatgattggc ctttctatga tccaccgatt tggcaagtgg gcatccccct gcagccgtgt
2161 gttgctctct tatcttcact ttctcacacc tccagtgctc aacccagttg tttataccat
2221 aaagaccaag cagatccgac agaggatttg gtgcatcttc cggtgtggtg gaagaagcat
2281 tgggcatatc cagggtcact aagaattctg cagatatcca gcacagtggc ggccgctcga
2341 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
2401 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
2461 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
2521 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
2581 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
2641 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
2701 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
2761 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
2821 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
2881 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
2941 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
3001 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
3061 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
3121 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
3181 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
3241 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
3301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
3361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
3421 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
3481 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
3541 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
3601 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
3661 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
3721 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
3781 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
3841 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
3901 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
3961 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
4021 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
4081 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
4141 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
4201 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
4261 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
4321 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
4381 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
4441 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
4561 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
4621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
4681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
4741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
4801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
4861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
5041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
5101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
5161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
5221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
5281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
5341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
5401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
5461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
5521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
5581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
5761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
5821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
5881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
5941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
6001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
6061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
6121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
6181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
6241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
6301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
6361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
6421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
6481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
6541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
6601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
6661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
6721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
6781 tc
//
pLYL76载体图谱质粒图谱pdf版和pLYL76载体序列质粒序列等相关资料下载
pLYL76质粒载体应用举例
- 上一篇：pLYL67
- 下一篇：没有了
