| 质粒名称: | PPRE-pNL1.3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84394 |
| 存储实验室: | Severine Degrelle, Thierry Fournier |
|
载体骨架基本信息 |
|
| 载体名称: | pNL1.3[secNluc] |
| 载体出品公司: | Promega |
| 空载体大小: | 3200 bp |
| 完整质粒大小: | 4600 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PPARG response element,PPRE,ApoCIII promotor |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1200 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | PPRE |
| 标签/融合蛋白: | secNluc（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | KpnI(未破坏) |
| 3’克隆位点: | BglII (未破坏) |
| 5’测序引物 | RVprimer3 |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PPRE-pNL1.3 was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 84394 ; http://n2t.net/addgene:84394 ; RRID:Addgene_84394) |
| 参考文献部分: | New Transcriptional Reporters to Quantify and Monitor PPARgamma Activity. Degrelle SA, Shoaito H, Fournier T. PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1. 10.1155/2017/6139107 PubMed 29225614 |
买家导航
PPREpNL1.3载体基本信息
PPREpNL1.3载体图谱质粒图谱和多克隆位点信息
PPREpNL1.3载体简介
PPREpNL1.3载体序列
LOCUS PPRE-pNL1.3 4303 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Secreted Luciferase reporter for PPARgamma activity.
ACCESSION .
VERSION .
KEYWORDS PPRE-pNL1.3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4303)
AUTHORS Degrelle SA, Shoaito H, Fournier T
TITLE New Transcriptional Reporters to Quantify and Monitor PPARgamma
Activity.
JOURNAL PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017
Nov 1.
PUBMED 29225614
REFERENCE 2 (bases 1 to 4303)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4303
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1253..1765
/codon_start=1
/product="NanoLuc(R) luciferase"
/label=Nluc
/note="human codon-optimized"
/translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
LCERILA"
polyA_signal 1814..1935
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1825..1844)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1879..1898
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2183..2200)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2354..2942)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2434..2453)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3142..4002)
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4107..4155
/note="synthetic polyadenylation signal"
misc_feature 4169..4260
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4209..4228
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 cagagccggg ccactttctc aggcctctga tttcatactg tggtgttagt tacttctgag
61 aggacagctt gctgccagag ctctattttt tatgttagag gctccttctg cctgcagact
121 ctgctgtctg ggaagggcac agcgttagga gggagaggga ggtgtgagtc cctccgtgga
181 cccgctgctt tgtacttctc tatctcattt ccttttcagc accactctgg gaaatcagta
241 ttccagcccc attttatcct cagaaaattg aggctctgag atgttatctc tgtgacctgg
301 gtcctattac gtgccaaagg catcatttaa gcctaagatg tcctggctcc aaggtgtcag
361 catctggaag acaggcgccc tcatcctgcc atccctgctg cggcttcact gtgggcccag
421 gggacatctc agccccgaga agggtcagcg gcccctcctg gaccaccgac tccccgcaga
481 actcctctgt gccctctcct caccagacct tgttcctccc agttgctccc acagccaggg
541 ggcagtgagg gctgctcttc ccccagcccc actgaggaac ccaggaaggt gaacgagaga
601 atcagtcctg gtgggggctg gggagggccc cagacatgag accagctcct cccccagggg
661 atgttatcag tgggtccaga gggcaaaata gggagcctgg tggagggagg ggcctgaaat
721 tccagtgtga aaggctgaga tgggcccgag gcccctggcc tatgtccaag ccatttcccc
781 tctcaccagc ctctccctgg ggagccagtc agctaggaag gaatgagggc tccccaggcc
841 caccccagtt cctgagctca tctgggctgc agggctggcg ggacagcagc gtggactcag
901 tctcctaggg atttcccaac tctcccgccc gcttgctgca tctggacacc ctgcctcagg
961 ccctcatctc cactggtcag caggtgacct ttgcccagcg ccctgggtcc tcagtgcctg
1021 ctgccctgga gatgatataa aacaggtcag aaccctcctg cctgtctgct cagttcatcc
1081 ctagaggcag gactctagag gatctgcgat cagatctggc ctcggcggcc aagcttggca
1141 atccggtact gttggtaaag ccaccatgaa ctccttctcc acaagcgcct tcggtccagt
1201 tgccttctcc ctgggcctgc tcctggtgtt gcctgctgcc ttccctgccc cagtcttcac
1261 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct
1321 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca
1381 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta
1441 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc
1501 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt
1561 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg
1621 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct
1681 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg
1741 gctgtgcgaa cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt
1801 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
1861 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
1921 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
1981 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
2041 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
2101 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
2161 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2761 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
2821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca
3121 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
3181 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
3241 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
3301 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
3361 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
3421 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
3481 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
3541 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
3601 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
3661 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
3721 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
3781 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
3841 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
3901 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
3961 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
4021 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa
4081 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
4141 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
4201 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
4261 ggcctaactg gccggtacca tgcctgcagg tcaattctga ggg
//
