| 质粒名称: | pSIE1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 136355 |
| 存储实验室: | Andrew Goodman |
|
载体骨架基本信息 |
|
| 载体名称: | pExchange_tdk |
| 载体出品公司: | Clontech |
| 空载体大小: | 2980bp |
| 完整质粒大小: | 6196bp |
| 载体类型: | Unspecified |
| 筛选标记: | Gentamicin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Pir1 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | Type VI effector bfe1 |
| 物种来源: | Bacteroides fragilis 638R |
| 插入大小: | 993bp |
| 基因突变: | ATG removed to add signal sequence before gene |
| 启动子: | P1TDP-GHO23 |
| 克隆方法: | Gibson Cloning |
| 插入基因名称2: | Type VI effector bte1 |
| 物种来源: | Bacteroides fragilis NCTC9343 |
| 插入大小: | 864bp |
| 基因突变: | ATG removed to add signal sequence before gene |
| 启动子: | P1TDP-GHO23 |
| 克隆方法: | Gibson Cloning |
| 插入基因名称3: | BT_4676、Periplasmic signal sequence |
| 物种来源: | Bacteroides thetaiotaomicron VPI5482 |
| 插入大小: | 63bp |
| 插入基因名称4: | tetR |
| 物种来源: | E. coli |
| 插入大小: | 624bp |
|
文献引用方法 |
|
| 材料和方法部分: | pSIE1 was a gift from Andrew Goodman (Addgene plasmid # 136355 ; http://n2t.net/addgene:136355 ; RRID:Addgene_136355) |
| 参考文献部分: | Genetic Manipulation of Wild Human Gut Bacteroides. Bencivenga-Barry NA, Lim B, Herrera CM, Trent MS, Goodman AL. J Bacteriol. 2020 Jan 15;202(3). pii: JB.00544-19. doi: 10.1128/JB.00544-19. Print 2020 Jan 15. 10.1128/JB.00544-19 PubMed 31712278 |
买家导航
pSIE1载体质粒基本信息
pSIE1质粒图谱载体图谱和pSIE1载体序列质粒序列多克隆位点信息
pSIE1质粒载体简介
pSIE1质粒序列载体序列
LOCUS pSIE1 6195 bp DNA circular SYN 14-APR-2025
DEFINITION Selection by Inducible Effectors.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6195)
AUTHORS Bencivenga-Barry NA, Lim B, Herrera CM, Trent MS, Goodman AL
TITLE Genetic Manipulation of Wild Human Gut Bacteroides.
JOURNAL J Bacteriol. 2020 Jan 15;202(3). pii: JB.00544-19. doi:
10.1128/JB.00544-19. Print 2020 Jan 15.
PUBMED 31712278
REFERENCE 2 (bases 1 to 6195)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J
Bacteriol."; date: "2020-01-15"; volume: "202(3). pii: JB.00544-19.
doi"; pages: " 10.1128/JB.00544-19. Print 2020 Jan 15"
COMMENT Sequence Label: pSIE1
FEATURES Location/Qualifiers
source 1..6195
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(join(30..821,822..890))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 653..672
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS complement(2161..2784)
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind 2692..2711
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
terminator 2937..2978
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 3074..3092
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3136..3154
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4208..4226
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4270..4288
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind complement(5377..5393)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5377..5393)
/label=pBluescriptSK
/note="For pBluescript vector"
rep_origin 5426..5814
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
oriT 5985..6094
/note="incP origin of transfer"
ORIGIN
1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
2161 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
2221 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
2281 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
2341 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
2401 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
2461 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
2521 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
2581 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
2641 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
2701 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
2761 tttactttta tctaatctag acataatgtt attttttaaa tgttgtgtga tcagtcctac
2821 tttgtttctt tcgacactgc aaatataaga acattatttg aaagttcaag tgaaacttta
2881 aattttaaca atagattaac cattgcaaac aaaacaaaaa aaaggtagcc caattgtaaa
2941 acgaaaggcc cagtctttcg actgagcctt tcgttttagt cgactttttg cacccgcttt
3001 ccaagagaag aaagccttgt taaattgact tagtgtaaaa gcgcagtact gcttgaccat
3061 aagaacaaaa aaatctctat cactgatagg gataaagttt ggaagataaa gctaaaagtt
3121 cttatctttg cagtctccct atcagtgata gagacgaaat aaagacatat aaaagaaaag
3181 acaccatgaa gaaaatttta tctttgcttg tgatggctat tgtagctata cagttctcat
3241 ttgccggcgg taaggaaata gaaataaaga aattgccaga gttcgaggct atggtgaatg
3301 ccggtaatac gacctatacg ggattggtag aaggcatcgg gtatgtgtat tggacaacag
3361 aaaccctata ttttatccgc actaatccag aacaattatg ggcgattcca aaatatcagc
3421 aaataccttt cccctatttt caaaggaaag atgcaatcat tgagaccaaa acattacata
3481 cactccatgt cttgtcaaaa gatgaactgt tgaaattgga ttacgatgcc tattatgcat
3541 tttatggtat cgtggaggag atgctaaaat ttattcatcg ggcggatgct attaaaagtt
3601 attgtgaaat acctcttccc ataataaaat ccaaaggagc actaaaggga aatgacgcaa
3661 gaaatgggat actatcatta ggtagtcaaa taaatgacca aatcggagct ccccttgatg
3721 cagcgaatat gctaatggat aataaacata taggcaaaat tggagatgga ttgtcgctta
3781 tttctattat agatgaggta ggtaatggtg aatattggtc agctgccgga gacattttac
3841 tgtttgcagc cggaaaaaca aaattaagtc cctatatgac tgtcataagt ttaggcacat
3901 ggatgtatga gacggacttg atgcaatgga gattagcatg tataaattat agcgattaca
3961 aaaaaacact aataaaatat cgagaattac aaaaaaaatt tgaaagtgga gacaaatctg
4021 tagaggaaaa gatgaatgaa tgtcacaaaa tactgaattc acattatata gagatgcaaa
4081 aaaatttagg taatctagga gttaaattct aactgcagtt tttgcacccg ctttccaaga
4141 gaagaaagcc ttgttaaatt gacttagtgt aaaagcgcag tactgcttga ccataagaac
4201 aaaaaaatct ctatcactga tagggataaa gtttggaaga taaagctaaa agttcttatc
4261 tttgcagtct ccctatcagt gatagagacg aaataaagac atataaaaga aaagacacca
4321 tgaagaaaat tttatctttg cttgtgatgg ctattgtagc tatacagttc tcatttgccg
4381 gcgcttatag aaatgaatat aaaacgcaaa gtgaatttaa tgtcatcttg gaacgggggg
4441 atgactatga aggatttgta gttggcttgg gatatacttg gatgagtagc aaggtaattc
4501 taccggtaaa tcaaaacgga tggagtccaa tatctcggaa tgtttcggtg gacgagagtt
4561 ttcatacgat agtctcagaa aggaaatacg atacctccca atatgcctat gaaaaaagtc
4621 tgatgcaaga tccaacaaaa gtttccgaaa aggtccgtga cttaatagtt aaaaacaaag
4681 gtaataatat cactgagata aatttaggcc aagaaaagca atatttgccc acggataata
4741 gtcagataag tattgtaatt actgacactg gcagtcggta cgaaattgtg attagtgcca
4801 cggataactc aaatggaaag acttatgagg caaagtatga gagtttgacg gacttggtgt
4861 cagcggtacg cgattctggt agcctgcctg ctgtaaataa ggaaggaccc aatctggaag
4921 gactggcagg gttaggattc ggaattgctg aaacagccgg aaattgggct gagaagatta
4981 tggataatcg aggtgcgtac ttacctaagc agatgcgttt ctcgcccaaa acgcttccgc
5041 cgattataag attgccttta gggaactatc aagtccccgc taaaggtatg agtagagtgc
5101 gtggagtagg taaagctttg ggatgggcag gaatggtgct tactggctat caagttgtgc
5161 gtgatgtgca aaatggtcga tttgccgtgg caggtacaag aattgccgta gcaggtttag
5221 cttatggcgt tacttttatt ccctatgtcg gttgggtctt agctatcggt atcggagtgg
5281 ctgattatac ttggggtgac gagttctatg actggataga caatagagct tctgaattgg
5341 aaatgtggtg ggacggtgta agattagcat tatgaggatc cactagttct agagcggccg
5401 ccaccgcggt ggaggggaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat
5461 tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac
5521 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
5581 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
5641 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
5701 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
5761 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac
5821 gccggacgca tcgtggccgg atcaattccg ttttccgctg cataaccctg cttcggggtc
5881 attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa
5941 agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa
6001 gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg
6061 ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc
6121 aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg
6181 atccccctcg aattg
//
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