| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-EF1α-DsRed-Monomer-N1, pLVX-EF1a-DsRed-Monomer-N1, pLVX-EF1alpha-DsRed-Monomer-N1 |
| 质粒类型: | 慢病毒表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | EF1α |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 9483 bp |
| 5' 测序引物及序列: | EF1a Forward: TCAAGCCTCAGACAGTGGTTC |
| 3' 测序引物及序列: | -- |
| 载体标签: | C-DsRed-Monomer |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素(Puromycin) |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系，293、CV-1、CHO等 |
| 备注: | pLVX-EF1α-DsRed-Monomer-N1载体表达C端DsRed-Monomer融合蛋白；DsRed-Monomer是DsRed的单体突变体，与DsRed相比，编码序列经过了人工优化，适用于在哺乳动物细胞中的高水平表达；EF1α启动子是高表达启动子，对于那些使用CMV启动子表达效果不理想的细胞系具有良好效果。 |
| 产品目录号: | 631990 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1a-DsRed-Monomer-N1 9483 bp DNA circular 22-MAR-2010
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 631990
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=Packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS(DNA\flap)
misc_feature 5452..6043
/label=WPRE
rep_origin complement(7351..8024)
/label=pUC\origin
CDS complement(8169..9165)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 6246..6882
/label=3'\LTR
misc_feature 3613..4290
/label=DsRed-Monomer
misc_feature 4310..4818
/label=PGK\Promoter
misc_feature 4839..5438
/label=Puro(R)
promoter 2185..3519
/label=EF1a\Promoter
misc_feature 3560..3599
/label=MCS
BASE COUNT 2244 a 2485 c 2603 g 2151 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac
3541 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
3601 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg
3661 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag
3721 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc
3781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc
3841 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc
3901 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc
3961 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg
4021 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg
4081 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac
4141 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac
4201 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac
4261 gccgaggccc gccactccgg ctcccagtag agcggccgcg actctagata attctaccgg
4321 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
4381 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
4441 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
4501 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
4561 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
4621 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
4681 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
4741 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
4801 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
4861 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
4921 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
4981 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
5041 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
5101 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
5161 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
5221 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
5281 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
5341 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
5401 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct
5461 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
5521 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
5581 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
5641 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
5701 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
5761 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
5821 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
5881 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
5941 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
6001 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc
6061 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
6121 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
6181 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
6241 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
6301 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
6361 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
6421 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
6481 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
6541 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
6601 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
6661 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
6721 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
6781 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
6841 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
6901 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
6961 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
7021 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
7081 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
7141 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
7201 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
7261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
7321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
7381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
7441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
7501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
7561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
7621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
7681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
7741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
7801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
7861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
7921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
7981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
8041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
8101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
8161 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
8221 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
8281 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
8341 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
8401 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
8461 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
8521 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
8581 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
8641 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
8701 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
8761 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
8821 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
8881 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
8941 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
9001 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
9061 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
9121 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
9181 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
9241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
9301 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
9361 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
9421 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
9481 agt
//
