pLVX-EF1α-DsRed-Monomer-N1

价格:1350元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-EF1α-DsRed-Monomer-N1, pLVX-EF1a-DsRed-Monomer-N1, pLVX-EF1alpha-DsRed-Monomer-N1
质粒类型: 慢病毒表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
启动子: EF1α
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9483 bp
5' 测序引物及序列: EF1a Forward: TCAAGCCTCAGACAGTGGTTC
3' 测序引物及序列: --
载体标签: C-DsRed-Monomer
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系,293、CV-1、CHO等
备注: pLVX-EF1α-DsRed-Monomer-N1载体表达C端DsRed-Monomer融合蛋白;DsRed-Monomer是DsRed的单体突变体,与DsRed相比,编码序列经过了人工优化,适用于在哺乳动物细胞中的高水平表达;EF1α启动子是高表达启动子,对于那些使用CMV启动子表达效果不理想的细胞系具有良好效果。
产品目录号: 631990
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-EF1α-DsRed-Monomer-N1载体图谱



pLVX-EF1α-DsRed-Monomer-N1多克隆位点

pLVX-EF1α-DsRed-Monomer-N1载体特征

载体简介

载体序列

LOCUS       pLVX-EF1a-DsRed-Monomer-N1   9483 bp    DNA   circular            22-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 631990
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS(DNA\flap)
     misc_feature    5452..6043
                     /label=WPRE
     rep_origin      complement(7351..8024)
                     /label=pUC\origin
     CDS             complement(8169..9165)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             6246..6882
                     /label=3'\LTR
     misc_feature    3613..4290
                     /label=DsRed-Monomer
     misc_feature    4310..4818
                     /label=PGK\Promoter
     misc_feature    4839..5438
                     /label=Puro(R)
     promoter        2185..3519
                     /label=EF1a\Promoter
     misc_feature    3560..3599
                     /label=MCS
BASE COUNT     2244 a      2485 c      2603 g      2151 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac 
     3541 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca 
     3601 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg 
     3661 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag 
     3721 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc 
     3781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc 
     3841 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc 
     3901 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc 
     3961 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg 
     4021 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg 
     4081 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac 
     4141 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac 
     4201 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac 
     4261 gccgaggccc gccactccgg ctcccagtag agcggccgcg actctagata attctaccgg 
     4321 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc 
     4381 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc 
     4441 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg 
     4501 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt 
     4561 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg 
     4621 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt 
     4681 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc 
     4741 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc 
     4801 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg 
     4861 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 
     4921 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 
     4981 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 
     5041 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 
     5101 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 
     5161 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 
     5221 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 
     5281 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 
     5341 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 
     5401 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct 
     5461 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     5521 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     5581 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     5641 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     5701 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     5761 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     5821 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 
     5881 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     5941 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     6001 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc 
     6061 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 
     6121 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 
     6181 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga 
     6241 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 
     6301 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat 
     6361 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag 
     6421 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg 
     6481 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg 
     6541 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac 
     6601 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc 
     6661 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag 
     6721 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 
     6781 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 
     6841 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 
     6901 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat 
     6961 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 
     7021 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     7081 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     7141 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     7201 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     7261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     7321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     7381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     7441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     7501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     7561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     7621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     7681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     7741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     7801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     7861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     7921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     7981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     8041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     8101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     8161 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     8221 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     8281 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     8341 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     8401 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     8461 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     8521 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     8581 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     8641 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     8701 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     8761 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     8821 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     8881 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     8941 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     9001 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     9061 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     9121 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 
     9181 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     9241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     9301 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 
     9361 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc 
     9421 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt 
     9481 agt 
//

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