pIRES

价格:800元

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载体基本信息

出品公司: Clontech
载体名称: pIRES
质粒类型: 哺乳动物细胞表达载体;双顺反子载体;双表达载体
启动子: CMV
表达水平:
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6092 bp
5' 测序引物:   CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物: pIRES-R:5'-GCCCTAGATGCATGCTCG-3'
载体标签:
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: 新霉素(Neomycin)
备注:
pIRES载体含有IRES元件,可以同时表达两个基因。
产品目录号: 631605
稳定性: 稳表达 或 瞬表达
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pIRES载体图谱和多克隆位点

载体简介

pIRES is a mammalian expression vector that allows high level expression of two genes of interest from the same bicistronic mRNA transcript. The vector contains the encephalomyocarditis virus (ECMV) internal ribosome entry site (IRES) flanked by two multiple cloning sites (MCS A and B), an arrangement that allows cap-independent translation of the gene cloned into MCS B (1–3). pIRES utilizes a partially disabled IRES sequence (1) that reduces the rate at which the gene cloned into MCS B is translated relative to that of MCS A.

Expression of the bicistronic transcript is driven by the constitutively active cytomegalovirus immediate early promoter (PCMV IE), located upstream of MCS A. An intervening sequence (IVS) known to enhance the stability of mRNA (4) is located between PCMV IE and MCS A, and is efficiently spliced out following transcription. SV40 polyadenylation signals downstream of MCS B direct proper processing of the 3' end of the mRNA. Bacteriophage T7 and T3 promoters are located upstream of MCS A and downstream of MCS B, respectively. pIRES includes a neomycin resistance gene (Neor) to aid in the selection of transfected cells. Neor is expressed from the SV40 enhancer/promoter, and a synthetic polyadenyla¬tion signal directs proper processing of the 3' end of the Neor mRNA. The SV40 origin al¬lows for replication in mammalian cells expressing the SV40 T antigen. The vector also contains an ampicillin resistance gene (Ampr), and a ColE1 origin of replication for se¬lection and propagation in E. coli, and an f1 origin for single-stranded DNA production.
 

载体序列

LOCUS       pIRES                   6107 bp    DNA     circular SYN 30-DEC-2014
DEFINITION  pIRES.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.biofeng.com/
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="pIRES"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        163..723
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    218..505
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        669..750
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    680..700
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     misc_feature    724..743
                     /vntifkey="21"
                     /label=pCEP_fwd_primer
     misc_feature    726..750
                     /vntifkey="21"
                     /label=LNCX_primer
     misc_feature    890..1022
                     /vntifkey="21"
                     /label=chim_int
     promoter        1067..1085
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    1136..1714
                     /vntifkey="21"
                     /label=IRES
     terminator      complement(1806..1925)
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    complement(1820..1839)
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      2133..2439
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(2713..2733)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2719..2934)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2731..2999
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2898..2975
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2960..2979
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             3098..3892
                     /vntifkey="4"
                     /label=ORF\frame\2
     gene            3101..3889
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(3407..3943)
                     /vntifkey="4"
                     /label=ORF\frame\2
     terminator      3956..4004
                     /vntifkey="43"
                     /label=synth_PA_terminator
     misc_feature    4164..4186
                     /vntifkey="21"
                     /label=pGEX_3_primer
     promoter        4345..4373
                     /vntifkey="30"
                     /label=AmpR_promoter
     gene            4415..5275
                     /gene="Ampicillin"
                     /vntifkey="60"
     CDS             4415..5275
                     /vntifkey="4"
                     /label=ORF\frame\2
     rep_origin      5430..6049
                     /vntifkey="33"
                     /label=pBR322_origin
BASE COUNT     1465 a      1547 c      1532 g      1563 t 
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt 
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa 
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact 
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac 
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact 
     1081 ataggctagc ctcgagaatt cacgcgtcga gcatgcatct agggcggcca attccgcccc 
     1141 tctccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 
     1201 taaggccggt gtgcgtttgt ctatatgtga ttttccacca tattgccgtc ttttggcaat 
     1261 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 
     1321 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 
     1381 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 
     1441 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 
     1501 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 
     1561 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 
     1621 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 
     1681 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg 
     1741 ggatcctcta gagtcgaccc gggcggccgc ttccctttag tgagggttaa tgcttcgagc 
     1801 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 
     1861 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 
     1921 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg 
     1981 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat 
     2041 ccgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 
     2101 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     2161 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     2221 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
     2281 tagggttccg atttagagct ttacggcacc tcgaccgcaa aaaacttgat ttgggtgatg 
     2341 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     2401 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     2461 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
     2521 tttaacaaat atttaacgcg aattttaaca aaatattaac gtttacaatt tcgcctgatg 
     2581 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag 
     2641 caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa 
     2701 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 
     2761 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
     2821 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 
     2881 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
     2941 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
     3001 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgattctt 
     3061 ctgacacaac agtctcgaac ttaaggctag agccaccatg attgaacaag atggattgca 
     3121 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 
     3181 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 
     3241 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 
     3301 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 
     3361 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 
     3421 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 
     3481 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 
     3541 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 
     3601 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 
     3661 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 
     3721 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 
     3781 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 
     3841 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 
     3901 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg gccgcaataa 
     3961 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagcgataag 
     4021 gatccgcgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc 
     4081 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 
     4141 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 
     4201 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 
     4261 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 
     4321 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 
     4381 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 
     4441 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 
     4501 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 
     4561 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 
     4621 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 
     4681 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 
     4741 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 
     4801 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 
     4861 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 
     4921 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 
     4981 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 
     5041 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 
     5101 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 
     5161 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 
     5221 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 
     5281 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     5341 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     5401 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     5461 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     5521 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     5581 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     5641 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     5701 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     5761 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     5821 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     5881 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     5941 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     6001 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     6061 gttcctggcc ttttgctggc cttttgctca catggctcga cagatct 
//

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