pIRES2-DsRed-Express2

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pIRES2-DsRed-Express2
质粒类型: 哺乳细胞表达载体 ; 双瞬反子载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 多克隆位点,限制性内切酶
启动子: CMV
载体大小: 5265 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 红色荧光蛋白DsRed-Express2
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5α, HB101
宿主细胞(系): 常规细胞系,293、CV-1、CHO等
备注: --
产品目录号: 632540
稳定性: 稳表达 或 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pIRES2-DsRed-Express2载体图谱



pIRES2-DsRed-Express2多克隆位点

pIRES2-DsRed-Express2载体特征

载体简介

pIRES2-DsRed-Express2 is a bicistronic expression vector that allows the simultaneous expression of your protein of interest and DsRed-Express2 from the same mRNA transcript.The vector is designed to allow efficient flow cytometric detection of transiently transfected mammalian cells expressing either DsRed-Express2 and a protein of interest, or DsRedExpress2 alone (at lower signal intensity).
DsRed-Express2 is a variant of the Discosoma sp. red fluorescent protein, DsRed (1).It retains the fast maturation and high photostability characteristic of its predecessor,DsRed-Express (2), and has been engineered (through additional amino acid substitutions) for increased solubility (3). Although it most likely forms the same tetrameric structure as wild-type DsRed, DsRed-Express2 displays a greatly reduced tendency to aggregate,resulting in reduced cyto- and phototoxicity, and making DsRed-Express2 much better suited for in vivo applications involving sensitive cells, such as primary or stem cells.DsRed-Express2 also exhibits extremely low residual green fluorescence, which allows cells expressing the protein to be effectively separated from other fluorescently labeled cell populations by flow cytometry
Bicistronic expression of the protein of interest and DsRed-Express2 is facilitated by the encephalomyocarditis virus (EMCV) internal ribosome entry site 2 (IRES2), located between the multiple cloning site (MCS) and the DsRed-Express2 coding region. This IRES allows cap-independent translation of DsRed-Express2 from an internal start site at the IRES/DsRed-Express2 junction (4, 5). Expression of the bicistronic transcript is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE) located just upstream of the MCS.
SV40 polyadenylation signals downstream of the DsRed-Express2 coding sequence direct proper processing of the 3' ends of the bicistronic mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 large T antigen, a pUC origin of replication for propagation in E. coli,and an f1 origin for single-stranded DNA production. This vector also has a neomycin-resistance cassette (Neor) that allows G418 selection of stably transfected eukaryotic cells (6). This cassette consists of the SV40 early promoter, a Tn5 kanamycin/neomycin resistance gene, and herpes simplex virus thymidine kinase (HSV TK) polyadenylation signals. A bacterial promoter upstream of this cassette allows kanamycin resistance in E. coli.
Use
When cloning into the pIRES2 DsRed-Express2 MCS, your gene of interest must contain an initiation codon (ATG) and a stop codon. Cells expressing the gene of interest can be quickly identified by screening for DsRed-Express2 fluorescence.pIRES2 DsRed-Express2 and its derivatives can be introduced into mammalian cells using any standard transfection method. Cells expressing DsRed-Express2 (excitation and emission maxima: 554 and 591,respectively) can be detected by flow cytometry or microscopy 24 hr after transfection. However, in some cases, up to 48 hr may be required for detection. If required, stable transfectants can be selected using G418. Please refer to the Living Colors® User Manual provided with this vector for additional information on detection of DsRed-Express2.

载体序列

LOCUS       pIRES2 DsRed-Express2	5265 bp 	DNA	SYN
DEFINITION  pIRES2 DsRed-Express2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="pIRES2 DsRed-Express2"
                     /mol_type="other DNA"
     promoter        10..562
                     /label="CMV_immearly_promoter"
     misc_feature    65..352
                     /label="CAG_enhancer"
     misc_feature    519..539
                     /label="CMV_fwd_primer"
     promoter        520..589
                     /label="CMV_promoter"
     misc_feature    666..1250
                     /label="IRES"
     CDS             1242..1931
                     /label="ORF frame 3"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     gene            1257..1928
                     /label="E2_Crimson (variant)"
                     /gene="E2_Crimson (variant)"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    complement(1434..1454)
                     /label="dsRed1_N_primer"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    1846..1869
                     /label="dsRed1_C_primer"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    2144..2163
                     /label="EBV_rev_primer"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     rep_origin      complement(2313..2619)
                     /label="f1_origin"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     promoter        2698..2726
                     /label="AmpR_promoter"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    complement(2792..2812)
                     /label="pBABE_3_primer"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    complement(2798..3013)
                     /label="SV40_enhancer"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     promoter        2810..3078
                     /label="SV40_promoter"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     rep_origin      2977..3054
                     /label="SV40_origin"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     misc_feature    3039..3058
                     /label="SV40pro_F_primer"
                     /translation="MATTMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG
                     TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV
                     LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY
                     ERAEARHHLFQ*"
     CDS             3161..3955
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            3164..3952
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(3470..4006)
                     /label="ORF frame 3"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      4130..4399
                     /label="TK_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     rep_origin      4547..5166
                     /label="pBR322_origin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
ORIGIN
    1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
   61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
  121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
  181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
  241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
  301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
  361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
  421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
  481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
  541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
  601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
  661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
  721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
  781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
  841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
  901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
  961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
 1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
 1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
 1141 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
 1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggata
 1261 gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt gcacatggag ggctccgtga
 1321 acggccacga gttcgagatc gagggcgagg gcgagggcaa gccctacgag ggcacccaga
 1381 ccgccaagct gcaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc
 1441 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca
 1501 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
 1561 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg caccttcatc taccacgtga
 1621 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actctgggct
 1681 gggagccctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca
 1741 aggcgctgaa gctgaagggc ggcggccact acctggtgga gttcaagtca atctacatgg
 1801 ccaagaagcc cgtgaagctg cccggctact actacgtgga ctccaagctg gacatcacct
 1861 cccacaacga ggactacacc gtggtggagc agtacgagcg cgccgaggcc cgccaccacc
 1921 tgttccagta ggcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
 1981 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
 2041 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
 2101 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
 2161 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
 2221 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
 2281 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
 2341 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
 2401 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
 2461 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
 2521 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
 2581 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
 2641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
 2701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
 2761 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
 2821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
 2881 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
 2941 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
 3001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
 3061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
 3121 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
 3181 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
 3241 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
 3301 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
 3361 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
 3421 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
 3481 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
 3541 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
 3601 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
 3661 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
 3721 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
 3781 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
 3841 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
 3901 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
 3961 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
 4021 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
 4081 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
 4141 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
 4201 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
 4261 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
 4321 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
 4381 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
 4441 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
 4501 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
 4561 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
 4621 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
 4681 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
 4741 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
 4801 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
 4861 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
 4921 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
 4981 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
 5041 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
 5101 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
 5161 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
 5221 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//

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