pHook-3

价格:3850元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pHOOK-3
质粒类型: 哺乳动物表达载体;Capture-Tec系统;免疫磁珠
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 6199 bp
5' 测序引物及序列: CMV fwd 5’ CGCAAATGGGCGGTAGGCGTG3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: IgK、HA tag (N-term);2XMyc tag (N-term)
载体抗性: 氨苄青霉素
筛选标记: 博来霉素
克隆菌株: DH5α
宿主细胞(系): 哺乳动物细胞
备注:
pHOOK3载体可以用于:  
 1.       The effect of dominant, negative mutations of growth regulating proteins or toxic gene products
2.       The acute effects of constitutive mutations
3.       The effect of anti-sense RNA or ribozymes
4.       Temporal events along a developmental or signaling pathway
5.       Activation or inactivation of downstream genes using differential display
6.       You may also use the pHook-3 System to isolate a homogeneous population of transfected cells for:
        a)        Immunoblot analysis of proteins
        b)        Isolation of DNA or RNA
        c)         Flow Cytometry

See pHOOK-1
 
产品目录号: V690-20
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pHOOK-3载体图谱



pHOOK-3多克隆位点

载体简介

pHook-3载体使用方法——免疫磁珠法


pHOOK-3使用流程



pHOOK-3使用流程



pHOOK-3使用流程



载体序列

LOCUS       Exported                6199 bp ds-DNA     circular SYN 13-AUG-2016
KEYWORDS    pHOOK-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Biofeng Lab.
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        29..358
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      209..344
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        787..834
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     promoter        826..844
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             854..1228
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1292..1426
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1590..2178)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2263..2566
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2567..2770
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2905..2923
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3261..3309
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4079..4341
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             4366..4428
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /note="Ig-kappa leader"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             4429..4455
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             5194..5223
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     CDS             5239..5268
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     promoter        complement(5460..5478)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    5504..5728
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5774..3
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 attatggtga ccggatccgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
       61 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      181 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      361 gctggctcga tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
      421 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
      481 taagtttaga gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
      541 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
      601 cgttttctgt tctgcgccgt tacaacttca aggtatgcgc tgggacctgg caggcggcat
      661 ctgggacccc taggaagggc ttgggggtcc tcgtgcccaa ggcagggaac atagtggtcc
      721 caggaagggg agcagaggca tcagggtgtc cactttgtct ccgcagctcc tgagcctgca
      781 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgactcacta
      841 taggagggcc accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
      901 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
      961 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     1021 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     1081 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     1141 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     1201 gcacttcgtg gccgaggagc aggactgacc gacgccgacc aacaccgccg gtccgacggc
     1261 ggcccacggg tcccaggggg gtcgacctcg aaacttgttt attgcagctt ataatggtta
     1321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccctc ggagatcgat
     1441 ctgggcccat gcggccggcc gcggatcgat gctcactcaa aggcggtaat acggttatcc
     1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2221 aacgaaaact cacgttaagg gattttggtc atggctagag tccgttacat aacttacggt
     2281 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     2341 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     2401 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2461 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2521 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2581 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2641 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2701 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2761 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2821 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2881 ggacctgcag cacgtgttga caattaatac gactcactat agggagaccc aagcttggta
     2941 ccagatcttt taaagctagc tctagagtac cgagctcgga tcccccgggc cagtgtgctg
     3001 gaagtaccga gctcgatcgt acccagcttg gaggttgcag ggtcgatgcg acgcaatcgt
     3061 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
     3121 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
     3181 gagggcaaag gaatagggga gatgggggag gctaactgaa acacggaagg agacaatacc
     3241 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
     3301 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     3361 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     3421 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
     3481 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
     3541 tattttgggc gttgcgtggg gtctggtcca cgactggact gagcagacag acccatggtt
     3601 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
     3661 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgc ccagtggtcg
     3721 accaattctc atgtttgaca gcttatcatc gcagatcctg agcttgtatg gtgcactctc
     3781 agtacaatct gctctgctgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     3841 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     3901 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     3961 agatatacgc gtatctgagg ggactagggt gtgtttaggc gcccagcggg gcttcggttg
     4021 tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg gagggggaaa
     4081 tgtagtctta tgcaatacac ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     4141 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     4201 gtgccttatt aggaaggcaa cagacaggtc tgacatggat tggacgaacc actgaattcc
     4261 gcattgcaga gataattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     4321 ttcaccacat tggtgtgcac ctccaagctg ggtacgatat ccaccatgga gacagacaca
     4381 ctcctgctat gggtactgct gctctgggtt ccaggttcca ctggtgacta tccatatgat
     4441 gttccagatt atgctggggc ccagccggcc atggccgagg tcaagctgca ggagtcaggg
     4501 ggaggcttag tgcagcctgg agggtcccgg aaactctcct gtgcagcctc tggattcact
     4561 ttcagtagct ttggaatgca ctgggttcgt caggctccag agaaggggct ggagtgggtc
     4621 gcatatatta gtagtggcag tagtaccatc tactatgcag acacagtgaa gggacgattc
     4681 accatctcca gagacaatcc caagaacacc ctgttcctgc aaatgaccag tctaaggtct
     4741 gaggacacgg ncatgtatta ctgtgcaaga gattacgggg cttattgggg ccaagggacc
     4801 acggncaccg tctcctcagg tggaggcggc tcaggcggag gtggctctgg cggtggcgga
     4861 tcggacattg agctcaccca gtctccagca atcatgtctg catctccagg ggagagggtc
     4921 accatgacct gcagtgccag ttcaagtgta aggtacatga actggttcca acagaagtca
     4981 ggcacctccc ccaaaagatg gatttatgac acatccaaac tgtcttctgg agtccctgct
     5041 cgcttcagtg gcagtgggtc tgggacctct tactctctca caatcagcag catggaggct
     5101 gaagatgctg ccacttacta ctgccagcag tggagtagta acccactcac gttcggtgct
     5161 gggaccaagc tggagctgaa acgggcggcc gcagaacaaa aactcatctc agaagaggat
     5221 ctgaatgggg ccgtcgacga acaaaaactc atctcagaag aggatctgaa tgctgtgggc
     5281 caggacacgc aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc
     5341 tcagccatcc tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt
     5401 tggcagaaga agccacgtta ggcggccgct cgagcatgca tctagctaga gggccctatt
     5461 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
     5521 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5581 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5641 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5701 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5761 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     5941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6121 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     6181 taacaaaaat ttaacgcga
//

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