pLP2

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pLP2
质粒类型: 哺乳动物细胞慢病毒表达载体
高拷贝/低拷贝: 高拷贝
启动子: RSV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4180 bp
5' 测序引物及序列: CMV-F:  CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
备注:
载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。

第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG(或者pCMV-VSVG)。在使用的时候,按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时,对于60mm的细胞培养皿来说,按照慢病毒基因质粒(3ug)和package mix(6 ug,也就是6 ul)的比例进行慢病毒质粒包装实验。
 
产品目录号: K4970-00
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLP2载体图谱


pLP2载体特征

载体简介

载体序列

LOCUS       Exported File           4180 bp ds-DNA    circular SYN 10-一月-2013
DEFINITION  Lentiviral packaging plasmid containing the HIV-1 rev gene.
ACCESSION   .
VERSION     .
KEYWORDS    pLP2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="synthetic DNA construct"
                     /lab_host="M"
                     /mol_type="other DNA"
     promoter        1..262
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             391..741
                     /codon_start=1
                     /product="HIV-1 Rev protein"
                     /note="rev"
     polyA_signal    850..971
                     /note="HIV-1 poly(A)"
                     /note="polyadenylation signal from the LTR of HIV-1"
     rep_origin      1173..1628
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1910..2014
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2015..2875
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3046..3634
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
      241 attcaccaca ttggtgtgca cctccaagct cgagctcgtt tagtgaaccg tcagatcgcc
      301 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      361 ccctcgaagc tagtcgatta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa
      421 gacctcctca aggcagtcag actcatcaag tttctctatc aaagcaaccc acctcccaat
      481 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
      541 cagatccatt cgattagtga acggatcctt agcacttatc tgggacgatc tgcggagcct
      601 gtgcctcttc agctaccacc gcttgagaga cttactcttg attgtaacga ggattgtgga
      661 acttctggga cgcagggggt gggaagccct caaatattgg tggaatctcc tacaatattg
      721 gagtcaggag ctaaagaata gtgctgttag cttgctcaat gccacagcta tagcagtagc
      781 tgaggggaca gatagggtta tagaagtagt acaagaagct tggcactggc cgtcgtttta
      841 caacgtcgtg atctgagcct gggagatctc tggctaacta gggaacccac tgcttaagcc
      901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      961 taactagaga tcaggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1021 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1081 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     1141 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     1201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1381 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     1441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     1501 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     1561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     1621 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     1681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3721 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3781 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3841 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     3901 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     3961 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     4021 aggaaacagc tatgacatga ttacgaattc gatgtacggg ccagatatac gcgtatctga
     4081 ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc
     4141 ctcaggatat agtagtttcg cttttgcata gggaggggga
//

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