pLVX-shRNA1

价格:1300元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-shRNA1 , pLVX shRNA1
质粒类型: 哺乳动物细胞慢病毒表达载体,shRNA,RNA干扰载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7758 bp
5' 测序引物及序列: LKO.1 5’:GACTATCATATGCTTACCGT
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
筛选标记: 嘌呤霉素
备注: 含有组成型人U6启动子的慢病毒shRNA载体
产品目录号: 632177
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-shRNA1载体图谱


pLVX-shRNA1多克隆位点

载体简介

Description

pLVX-shRNA1 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is drivenby the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA1 can either be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.

pLVX-shRNA1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA (1), leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-shRNA1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, thiselement creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (3).

In addition to lentiviral elements, pLVX-shRNA1 contains a puromycin resistance gene (Puror)under the control of the murine phosphoglycerate kinase promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

Use
pLVX-shRNA1 is available in the Lenti-X™ shRNA Expression System (Cat. No. 632177). Before it can be transduced into target cells, the vector must be cotransfected into 293T cells with our Lenti-X HTX Packaging System (Cat. Nos. 631247 and 631249) and packaged into viral particles. This packaging system allows the safe production of high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).

载体序列

LOCUS       pLVX-shRNA1   7757 bp    DNA   circular             8-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632177,632178
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     promoter        2457..2965
                     /label=PGK\promoter
     CDS             2986..3585
                     /label=Puro(R)
     misc_feature    2187..2444
                     /label=Human\U6\promoter
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     misc_feature    3679..4270
                     /label=WPRE
     rep_origin      complement(5578..6251)
                     /label=pUC\origin
     CDS             complement(6396..7392)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             551..635
                     /label=U5
     misc_binding    636..653
                     /label=PBS
     LTR             4473..5109
                     /label=3'\LTR
BASE COUNT     1968 a      1930 c      2022 g      1837 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt 
     2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 
     2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 
     2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 
     2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccggacaa gcttcgaatt 
     2461 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 
     2521 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 
     2581 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 
     2641 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 
     2701 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 
     2761 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 
     2821 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 
     2881 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 
     2941 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag 
     3001 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 
     3061 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 
     3121 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 
     3181 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 
     3241 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 
     3301 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 
     3361 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 
     3421 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 
     3481 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 
     3541 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac 
     3601 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggcgtctcg agtctagagg 
     3661 gcccgacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 
     3721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 
     3781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 
     3841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 
     3901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 
     3961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     4021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 
     4081 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 
     4141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 
     4201 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 
     4261 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta 
     4321 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg 
     4381 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag 
     4441 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa 
     4501 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga 
     4561 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 
     4621 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 
     4681 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg 
     4741 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 
     4801 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 
     4861 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc 
     4921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 
     4981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 
     5041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 
     5101 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 
     5161 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc 
     5221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     5281 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     5341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     5401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     5461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     5521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     5581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     5641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     5701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     5761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     5821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     5881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     5941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     6001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     6061 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     6121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     6181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     6241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     6301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     6361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     6421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     6481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     6541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     6601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     6661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     6721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     6781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     6841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     6901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     6961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     7021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     7081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     7141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     7201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     7261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     7321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     7381 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 
     7561 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg 
     7621 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat 
     7681 ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt 
     7741 actacaaact tagtagt 
//

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