| 出品公司: | Thermofisher |
|---|---|
| 载体名称: | pTet-tTAK；pTettTAK |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Tet |
| 载体大小: | 6.2 kb |
| 5' 测序引物及序列: | pBluescriptKS primer:TCGAGGTCGACGGTATC |
| 3' 测序引物及序列: | pBluescriptSK Primer:TCTAGAACTAGTGGATC |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 这个质粒含Tet操纵子，这个操纵子控制质粒上面的CMV启动子，所以当这个质粒与激活质粒pTet-Splice共表达的时候，可以控制pTet-splice质粒上面的目的蛋白在Doxycycline多西环素（一种四环素）的诱导下进行表达。所以这个质粒是哺乳细胞四环素诱导表达质粒。 |
| 产品目录号: | #217569 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 可诱导 |
| 病毒/非病毒: | 非病毒 |
买家导航
pTet-tTAK载体 pTettTAK质粒基本信息
pTet-tTAK质粒图谱 pTettTAK载体图谱和pTet-tTAK载体序列 pTettTAK质粒序列多克隆位点信息
pTet-tTAK质粒 pTettTAK载体简介
pTet-tTAK质粒序列 pTettTAK载体序列
LOCUS pTet-tTAK 6206 bp ds-DNA circular SYN 15-FEB-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6206)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, February 15, 2019 from SnapGene Viewer 3.2.1
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6206
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 15..285
/bound_moiety="tetracycline repressor TetR"
/note="tetracycline response element"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 318..355
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 484..500
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
CDS 515..1525
/codon_start=1
/product="tetracycline-controlled transactivator,
comprising a fusion of the tetracycline repressor TetR with
the C-terminal activation domain of herpes simplex virus
VP16"
/note="tTA"
/note="In the Tet-Off(R) system, tTA binds to the
Tet-responsive element and stimulates transcription only in
the absence of tetracycline or doxycycline."
primer_bind complement(1562..1578)
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
intron 1781..1846
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS 1976..1996
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
polyA_signal 2421..2555
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(3347..3365)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3375..3391)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3533..3988
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4014..4118
/gene="bla"
/note="AmpR promoter"
CDS 4119..4979
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 5150..5738
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6026..6047
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6062..6092
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6100..6116
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6124..6140
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 6161..6179
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
421 ccgggaccga tccagcctcc gcggccccga attcgagctc ggtacccggg gatcctctag
481 agtcgaggtc gacggtatcg ataagcttgc caccatggct tctagattag ataaaagtaa
541 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
601 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
661 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
721 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
781 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
841 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
901 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
961 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
1021 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
1081 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
1141 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
1201 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
1261 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
1321 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
1381 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
1441 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
1501 tggaattgac gagtacggtg ggtagggggc gcgaggatca tcgaattcct gcagcccggg
1561 ggatccacta gttctagagc actgcgatga gtggcagggc ggggcgtaat ttttttaagg
1621 cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg
1681 aatggcagaa attcgccgga ggcggatctt tgtgaaggaa ccttacttct gtggtgtgac
1741 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
1801 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
1861 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
1921 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
1981 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
2041 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
2101 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
2161 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
2221 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
2281 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
2341 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
2401 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
2461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
2521 ccaaactcat caatgtatct tatcatgtct ggatccccag gaagctcctc tgtgtcctca
2581 taaaccctaa cctcctctac ttgagaggac attccaatca taggctgccc atccaccctc
2641 tgtgtcctcc tgttaattag gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca
2701 gaccgctttc taagggtaat tttaaaatat ctgggaagtc ccttccactg ctgtgttcca
2761 gaagtgttgg taaacagccc acaaatgtca acagcagaaa catacaagct gtcagctttg
2821 cacaagggcc caacaccctg ctcatcaaga agcactgtgg ttgctgtgtt agtaatgtgc
2881 aaaacaggag gcacattttc cccacctgtg taggttccaa aatatctagt gttttcattt
2941 ttacttggat caggaaccca gcactccact ggataagcat tatccttatc caaaacagcc
3001 ttgtggtcag tgttcatctg ctgactgtca actgtagcat tttttggggt tacagtttga
3061 gcaggatatt tggtcctgta gtttgctaac acaccctgca gctccaaagg ttccccacca
3121 acagcaaaaa aatgaaaatt tgacccttga atgggttttc cagcaccatt ttcatgagtt
3181 ttttgtgtcc ctgaatgcaa gtttaacata gcagttaccc caataacctc agttttaaca
3241 gtaacagctt cccacatcaa aatatttcca caggttaagt cctcatttaa attaggcaaa
3301 ggaattgctc tagagcggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg
3361 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
3421 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
3481 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
3541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
3601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
3661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
3721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
3781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
3841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
3901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
3961 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
4561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
4621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
4681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
4741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
4801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
4861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
4921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
4981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
5521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
5581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
5641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
5701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
5761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
5821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
5881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
5941 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
6001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
6061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
6121 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
6181 aacaaaagct gggtaccggg cccccc
//
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pTet-tTAK质粒 pTettTAK载体应用举例
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