pTet-tTAK

价格:6500元

联系方式:I47-825O-882O

买家导航

pTettTAK载体基本信息

出品公司: Thermofisher
载体名称: pTet-tTAK;pTettTAK
质粒类型: 哺乳动物细胞载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: Tet
载体大小: 6.2 kb
5' 测序引物及序列: pBluescriptKS primer:TCGAGGTCGACGGTATC 
3' 测序引物及序列: pBluescriptSK Primer:TCTAGAACTAGTGGATC
载体抗性: 氨苄青霉素
克隆菌株: DH5α 等
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 这个质粒含Tet操纵子,这个操纵子控制质粒上面的CMV启动子,所以当这个质粒与激活质粒pTet-Splice共表达的时候,可以控制pTet-splice质粒上面的目的蛋白在Doxycycline多西环素(一种四环素)的诱导下进行表达。所以这个质粒是哺乳细胞四环素诱导表达质粒。
产品目录号: #217569
稳定性: 稳表达
组成型/诱导型: 可诱导
病毒/非病毒: 非病毒

pTettTAK载体质粒图谱和多克隆位点信息

pTet-tTAK载体图谱
pTet-tTAK载体图谱

pTettTAK载体简介

pTettTAK载体序列

LOCUS       pTet-tTAK     6206 bp ds-DNA     circular SYN 15-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, February 15, 2019 from SnapGene Viewer 3.2.1
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    15..285
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        318..355
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     484..500
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             515..1525
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /note="tTA"
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
     primer_bind     complement(1562..1578)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          1781..1846
                     /note="small t intron"
                     /note="simian virus 40 (SV40) small t antigen intron"
     CDS             1976..1996
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    2421..2555
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(3347..3365)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3375..3391)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3533..3988
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4014..4118
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4119..4979
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      5150..5738
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6026..6047
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6062..6092
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6100..6116
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6124..6140
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6161..6179
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgagta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      421 ccgggaccga tccagcctcc gcggccccga attcgagctc ggtacccggg gatcctctag
      481 agtcgaggtc gacggtatcg ataagcttgc caccatggct tctagattag ataaaagtaa
      541 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
      601 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
      661 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
      721 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
      781 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
      841 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
      901 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
      961 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     1021 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     1081 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     1141 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
     1201 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
     1261 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
     1321 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     1381 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
     1441 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     1501 tggaattgac gagtacggtg ggtagggggc gcgaggatca tcgaattcct gcagcccggg
     1561 ggatccacta gttctagagc actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     1621 cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg
     1681 aatggcagaa attcgccgga ggcggatctt tgtgaaggaa ccttacttct gtggtgtgac
     1741 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     1801 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     1861 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     1921 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     1981 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     2041 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     2101 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     2161 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     2221 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     2281 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     2341 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2401 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2521 ccaaactcat caatgtatct tatcatgtct ggatccccag gaagctcctc tgtgtcctca
     2581 taaaccctaa cctcctctac ttgagaggac attccaatca taggctgccc atccaccctc
     2641 tgtgtcctcc tgttaattag gtcacttaac aaaaaggaaa ttgggtaggg gtttttcaca
     2701 gaccgctttc taagggtaat tttaaaatat ctgggaagtc ccttccactg ctgtgttcca
     2761 gaagtgttgg taaacagccc acaaatgtca acagcagaaa catacaagct gtcagctttg
     2821 cacaagggcc caacaccctg ctcatcaaga agcactgtgg ttgctgtgtt agtaatgtgc
     2881 aaaacaggag gcacattttc cccacctgtg taggttccaa aatatctagt gttttcattt
     2941 ttacttggat caggaaccca gcactccact ggataagcat tatccttatc caaaacagcc
     3001 ttgtggtcag tgttcatctg ctgactgtca actgtagcat tttttggggt tacagtttga
     3061 gcaggatatt tggtcctgta gtttgctaac acaccctgca gctccaaagg ttccccacca
     3121 acagcaaaaa aatgaaaatt tgacccttga atgggttttc cagcaccatt ttcatgagtt
     3181 ttttgtgtcc ctgaatgcaa gtttaacata gcagttaccc caataacctc agttttaaca
     3241 gtaacagctt cccacatcaa aatatttcca caggttaagt cctcatttaa attaggcaaa
     3301 ggaattgctc tagagcggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg
     3361 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3421 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3481 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3541 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3601 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3661 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3721 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3781 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3841 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3901 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3961 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5941 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6001 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6061 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6121 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6181 aacaaaagct gggtaccggg cccccc
//

pTettTAK载体质粒图谱pdf版和相关资料下载

pTettTAK载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng
欢迎您的访问和咨询....