pFGC5941

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: --
载体名称: pFGC5941
质粒类型: 植物载体;RNAi载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: 35S
载体大小: 11406 bp
5' 测序引物及序列: 35S promoter: CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列: --
载体标签: --
载体抗性: 卡那霉素
筛选标记: 草胺膦、草丁膦
克隆菌株: HB101等菌株
宿主细胞(系): 农杆菌/植物细胞
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

> >

载体质粒图谱和多克隆位点信息

pFGC5941载体图谱



pFGC5941多克隆位点1

pFGC5941多克隆位点2

载体简介

载体序列

LOCUS       Exported               11406 bp ds-DNA     circular SYN 27-JUL-2016
DEFINITION  Agrobacterium binary vector for expressing dsRNA, with two multiple 
            cloning sites separated by an intron.
ACCESSION   AY310901
VERSION     .
KEYWORDS    pFGC5941
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Kerschen A, Napoli CA, Jorgensen RA, Muller AE.
  TITLE     Effectiveness of RNA interference in transgenic plants.
  JOURNAL   FEBS Lett. 2004;566:223-8.
  PUBMED    15147899
REFERENCE   2  (bases 1 to 11406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, July 27, 2016 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     The GenBank sequence was corrected by inserting a G at position 
            7514.
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /note="BlpR"
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2305..2650
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_signal     2680..2734
                     /note="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    2734..2764
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     intron          2767..4115
                     /gene="chsA"
                     /note="chsA intron"
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    4122..4160
                     /note="MCS 2"
                     /note="multiple cloning site"
     terminator      4177..4884
                     /note="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    5128..5152
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6452..7081
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7510..8583
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8649..8843
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9187..9327
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9513..10101)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10188..10982)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agttagatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactag ctcagatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagactcc tttgccccgg agattacaat ggacgatttc
     1741 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     1801 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     1861 agagaggcct acgcagcagg tctcatcaag acgatctacc cgagtaacaa tctccaggag
     1921 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     1981 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     2041 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     2101 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     2161 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     2221 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     2281 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     2341 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     2401 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     2461 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     2521 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     2581 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     2641 ggagaggaca cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa
     2701 caacaaacaa cattacaatt acatttacaa ttaccatggg gcgcgcccaa tcgatgattt
     2761 aaatgtgtaa gaatttctta tgttacatta ttacattcaa cgttttatct taattggctc
     2821 ttcatttgat tgaaatttga caattatttc ttgttttttt ttttgtcaca ctctttttgg
     2881 gttggggtgg ccgacgaatt gtgggaaggt agaaagaggg gaggactttt gttatactcc
     2941 attagtaatt actgtttccg tttcaattta tgtgacaata tttccttttt agtcggttcc
     3001 aaaagaaaat gtcagcatta taaacaattt aattttgaaa ttacaatttt gccattaata
     3061 aaatgattta caaccacaaa agtatctatg agcctgtttg ggtgggctta taagcagctt
     3121 attttaagtg gcttataagt caaaaagtga cantttttga gaagttagaa aatcctaact
     3181 tctcaaaaag tagcttttaa gccacttatg acttataagt ccaaaaattt ttaagttacc
     3241 aaacatatat taatgggttt ataagcttat aagccacttt taagctcacc caaacgggtt
     3301 ctatgtctca ctttagacta caaattttaa aagtcttcat ttatttctta atctccgtgg
     3361 cgagtnaaac tataacacat aaagtgaaac ggagggaata agatggagtc ataaactaat
     3421 ccaaatctat actctctccg ttaatttgtt ttttagtttg atttggtaca ttaataaaac
     3481 agatttttcg aaggttataa acacagacag atgtttccca gcgagctagc aaaattccaa
     3541 gatttctgtc gaaaattcgt gtgtttctag ctagtacttg atgttatctt taacctttta
     3601 gtaatttttt gtccttttct ttctattttt catcttacaa tgaattatga gcaagttcct
     3661 taagtagcat cacacgtgag atgtttttta tgatattgac taaatccaat ctttaccatt
     3721 ccttaactag taaaatacaa cacatgttaa ttgatacatt gcttaacact gaggttagaa
     3781 aattttagaa attagttgtc caaatgcttt gaaattagaa atctttaatc ccttattttt
     3841 ttttaaaatg ttttttctca ctccaaagaa agagaaactg acatgaaagc tcaaaagatc
     3901 atgaatctta ctaactttgt ggaactaaat gtacatcaga atgtttctga catgtgaaaa
     3961 tgaaagctct taattttctt cttttattta ttgagggttt ttgcatgcta tgcattcaat
     4021 ttgagtactt taaagcacct ataaacactt acttacactt gccttggagt ttatgtttta
     4081 gtgttttctt cacatctttt ttggtcaatt tgcaggtatt tggatcctag gtgagtctag
     4141 agagttaatt aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac
     4201 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
     4261 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
     4321 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
     4381 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
     4441 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
     4501 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
     4561 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
     4621 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
     4681 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
     4741 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
     4801 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
     4861 cgacaacatg tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt
     4921 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4981 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     5041 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     5101 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     5161 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt
     5221 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
     5281 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     5341 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     5401 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     5461 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     5521 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     5581 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     5641 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     5701 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     5761 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     5821 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     5881 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     5941 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     6001 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     6061 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     6121 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     6181 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     6241 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     6301 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     6361 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     6421 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     6481 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     6541 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     6601 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     6661 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     6721 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     6781 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     6841 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     6901 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     6961 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     7021 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     7081 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     7141 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     7201 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     7261 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     7321 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     7381 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     7441 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     7501 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     7561 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
     7621 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     7681 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
     7741 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     7801 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     7861 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
     7921 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     7981 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     8041 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     8101 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     8161 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     8221 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     8281 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     8341 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     8401 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     8461 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     8521 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     8581 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt
     8641 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     8701 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     8761 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     8821 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
     8881 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
     8941 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
     9001 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
     9061 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     9121 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     9181 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     9241 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     9301 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     9361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10141 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca
    10201 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
    10261 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
    10321 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
    10381 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
    10441 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
    10501 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
    10561 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
    10621 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
    10681 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
    10741 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
    10801 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
    10861 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
    10921 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
    10981 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
    11041 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
    11101 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
    11161 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    11221 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    11281 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    11341 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    11401 ggctgg
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng