YCplac111

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: YCplac111
质粒类型: 酿酒酵母表达载体;中心粒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lacZ
载体大小: 6112 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: LEU3
表达菌株: 酿酒酵母、白色念珠菌
备注: --
产品目录号: 87587
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

YCplac111载体图谱



YCplac111 多克隆位点

载体简介

载体序列

LOCUS       YCplac111	6112 bp 	DNA	SYN
DEFINITION  YCplac111
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="YCplac111"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     misc_feature    504..1356
                     /label="CEN4"
     CDS             complement(2066..2545)
                     /label="ORF frame 1"
                     /translation="MKFIIETTRNYKMEYVHRVELILMFDSLSSIVQLHGDESWQEYQ
                     EFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQ
                     ESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK*"
     gene            complement(2589..3683)
                     /label="LEU2 (variant)"
                     /gene="LEU2 (variant)"
                     /translation="MKFIIETTRNYKMEYVHRVELILMFDSLSSIVQLHGDESWQEYQ
                     EFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDWVGRQ
                     ESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNAKK*"
     CDS             complement(2589..3695)
                     /label="ORF frame 3"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     promoter        4241..4269
                     /label="AmpR_promoter"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     gene            4311..5171
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
                     FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
                     ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
                     WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
                     PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
                     SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
                     KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     CDS             4311..5171
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5326..5945
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
  541 tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat
  601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
  661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
  721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
  781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
  841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
  901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
  961 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa
 1021 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
 1081 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
 1141 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
 1201 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
 1261 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
 1321 acagttttac agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga
 1381 gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
 1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
 1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
 1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
 1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
 1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
 1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
 1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
 1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
 1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
 1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
 2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
 2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
 2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
 2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
 2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
 2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
 2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatcgatg
 2461 ataagctgtc aaacatgaga attaattcta ccctatgaac atattccatt ttgtaatttc
 2521 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
 2581 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
 2641 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
 2701 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
 2761 gcagacaaga tagtggcgat agggtcaacc ttattctttg gcaaatctgg agcagaaccg
 2821 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
 2881 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
 2941 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
 3001 gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt
 3061 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
 3121 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
 3181 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
 3241 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
 3301 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
 3361 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
 3421 ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcaacc
 3481 ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcatc gatagcagca
 3541 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
 3601 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
 3661 acgatcttct taggggcaga cataggggca gacattagaa tggtatatcc ttgaaatata
 3721 tatatatatt gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc
 3781 acctattgga aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc
 3841 aagcatttag tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcctctcacc
 3901 tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa
 3961 ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc tgtgtgttct
 4021 cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat
 4081 acctgagtat tcccacagtt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
 4141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
 4201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
 4261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
 4321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
 4381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
 4441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
 4501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
 4561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
 4621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
 4681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
 4741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
 4801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
 4861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
 4921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
 4981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
 5041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
 5101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
 5161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
 5221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
 5281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
 5341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
 5401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
 5461 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
 5521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
 5581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
 5641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
 5701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
 5761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
 5821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
 5881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
 5941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
 6001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
 6061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga

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