pGL4.73[hRluc/SV40]

价格:1500元

联系方式:I47-825O-882O

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pGL4.73载体基本信息

出品公司: Promega
载体名称: pGL4.73 ; pGL4.73[hRluc/SV40]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: SV40
载体大小: 3921 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRluc
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.73[luc2P]载体是信号通路报告载体;含海肾 荧光素酶报告基因hRluc。
产品目录号: E6911
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.73载体质粒图谱和多克隆位点信息

pGL4.73载体图谱



pGL4.73 载体特征

pGL4.73载体简介

pGL4.73载体简介
The pGL4.73[hRluc/SV40] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.73[hRluc/SV40] Vector contains the hRluc reporter gene and an SV40 early enhancer/promoter and can be used as an expression control or a co-reporter vector.

pGL4.73载体序列

LOCUS       AY738229                3921 bp    DNA     circular SYN 08-NOV-2004
DEFINITION  Renilla luciferase reporter vector pGL4.73[hRluc/SV40], complete
            sequence.
ACCESSION   AY738229
VERSION     AY738229.1  GI:55535639
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.73[hRluc/SV40]
  ORGANISM  Renilla luciferase reporter vector pGL4.73[hRluc/SV40]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Almond,B., Chauvin,F. and Browning,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Renilla luciferase reporter vector
                     pGL4.73[hRluc/SV40]"
                     /mol_type="other DNA"
                     /db_xref="taxon:296666"
     regulatory      51..469
                     /regulatory_class="promoter"
                     /note="SV40 early"
     CDS             499..1434
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase hRluc"
                     /protein_id="AAV52883.1"
                     /db_xref="GI:55535640"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1466..1687
                     /note="SV40 late poly(A) region"
     primer_bind     1755..1774
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      2012
                     /note="ColE1-derived"
     gene            complement(2803..3663)
                     /gene="ampR"
     CDS             complement(2803..3663)
                     /gene="ampR"
                     /note="AmpR"
                     /codon_start=1
                     /transl_table=11
                     /product="synthetic beta-lactamase"
                     /protein_id="AAV52884.1"
                     /db_xref="GI:55535641"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3768..3921
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic; transcriptional pause site"
     primer_bind     complement(3870..3889)
                     /note="reporter vector primer 3 (RVPrimer3)"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcagatct gcgcagcacc
       61 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
      121 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gcaatccggt
      481 actgttggta aagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg
      541 atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc
      601 aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct
      661 gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc
      721 atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc
      781 ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc
      841 atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa
      901 gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac
      961 gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg
     1021 gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg
     1081 gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg
     1141 cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc
     1201 cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc
     1261 atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct
     1321 aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa
     1381 atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag
     1441 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     1501 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     1561 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     1621 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     1681 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
     1741 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     1801 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     1861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2761 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     2821 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     2881 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     2941 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     3001 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     3061 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     3121 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     3181 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     3241 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     3301 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     3361 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     3421 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     3481 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     3541 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     3601 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     3661 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     3721 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
     3781 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     3841 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     3901 caggtgccag aacatttctc t
//

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