| 质粒名称: | pAAV-hSyn-GRAB-gDA3mut |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000418 |
| 产品用途: | Expresses the DA control sensor GRAB_gDA3mut in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_gDA3mut |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
Zhuo Y., Luo B., Yi X., Dong H., Miao X., Wan J., Williams J.T., Campbell M.G., Cai R., Qian T., Li F., Weber S.J., Wang L., Li B., Wei Y., Li G., Wang H., Zheng Y., Zhao Y., Wolf M.E., Zhu Y., Watabe-Uchida M., Li Y.
Nat. Methods 2023 214 21, 680–691 (2023)
|
pAAV-hSyn-GRAB-gDA3mut
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-hSyn-GRAB-gDA3mut载体质粒基本信息
pAAV-hSyn-GRAB-gDA3mut质粒图谱载体图谱和pAAV-hSyn-GRAB-gDA3mut载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB-gDA3mut质粒载体简介
pAAV-hSyn-GRAB-gDA3mut质粒序列载体序列
LOCUS pAAV-hSyn-GRAB-gDA3mut 6551 bp DNA circular SYN 23-JAN-2024
DEFINITION Expresses the genetically-encoded fluorescent dopamine (DA) control
sensor GRAB_gDA3mut in neurons.
ACCESSION .
VERSION .
KEYWORDS pAAV-hSyn-GRAB-gDA3mut
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6551)
TITLE Improved dual-color GRAB sensors for monitoring dopaminergic
activity in vivo
REFERENCE 2 (bases 1 to 6551)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6551
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 15..144
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 145..588
/label=hSyn
regulatory 595..604
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 601..2667
/codon_start=1
/label=gDA3mut
/translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGRTLNTSAMDGTGL
VVERDFSVRILTACFLSLLILSTLLGNTLVCAAVIRFRHLRSKVTNFFVISLAVSDLLV
AVLVMPWKAVAEIAGFWPFGSFCNIWVAFDIMCSTASILNLCVISVDRYWAISSPARYE
RKMTPKAAFILISVAWTLSVLISFIPVQLSWHKAKPTSPSDGNATSLAETIDNCDSSLS
RTYAISASVIPFYIPVAIMIVLYTRIYRIAQKQLNSLIYVYIKADKQKNGIKANFGIRH
NIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAG
ITLGMDELYKGGTGGLMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFK
NDGFYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNNHDQLKRETKVLKTLS
VIMGVFVCCWLPFFILNCILPFCGSGETQPFCIDSNTFDVFVWFGWANSSLNPIIYAFN
ADFRKAFSTLLGCYRLCPATNNAIETVSINNNGAAMFSSHHEPRGSISKECNLVYLIPH
AVGSSEDLKKEEAAGIARPLEKLSPALSVILDYDTDVSLEKIQPITQNGQHPT"
sig_peptide 601..663
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 664..688
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind 1587..1608
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1714..1735)
/label=EGFP-N
/note="EGFP, reverse primer"
misc_feature 2692..3280
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2745..2765)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3163..3174)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3312..3788
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3511..3530)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3828..3968
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3828..3957
/label=AAV2 ITR
rep_origin 4043..4498
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4130..4149)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4340..4361
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4515..4534)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4634..4656
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4694..4712)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4780..4884
/gene="bla"
/label=AmpR promoter
CDS 4885..5745
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5103..5122)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5916..6504
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6405..6424
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc
61 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
121 ccaactccat cactaggggt tcctgcggcc gcacgcgtat gaggcggggt gggggtgcct
181 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
241 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
301 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
361 gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc
421 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
481 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
541 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgagaaac cggtgccacc
601 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
661 gacacaagtt tgtacaaaaa agttggcacc accggtagga ctctgaacac ctctgccatg
721 gacgggactg ggctggtggt ggagagggac ttctctgttc gtatcctcac tgcctgtttc
781 ctgtcgctgc tcatcctgtc cacgctcctg gggaacacgc tggtctgtgc tgccgttatc
841 aggttccgac acctgcggtc caaggtgacc aacttctttg tcatctcctt ggctgtgtca
901 gatctcttgg tggccgtcct ggtcatgccc tggaaggcag tggctgagat tgctggcttc
961 tggccctttg ggtccttctg taacatctgg gtggcctttg acatcatgtg ctccactgca
1021 tccatcctca acctctgtgt gatcagcgtg gacaggtatt gggctatctc cagccctgcc
1081 cggtatgaga gaaagatgac ccccaaggca gccttcatcc tgatcagtgt ggcatggacc
1141 ttgtctgtac tcatctcctt catcccagtg cagctcagct ggcacaaggc aaaacccaca
1201 agcccctctg atggaaatgc cacttccctg gctgagacca tagacaactg tgactccagc
1261 ctcagcagga catatgccat ctcagcctct gtaataccct tttacatccc tgtggccatc
1321 atgattgtct tgtacaccag gatctacagg attgctcaga aacagctgaa ttcactcatt
1381 tatgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt cgggatccgc
1441 cacaacatcg aggacggtgg cgtgcagctc gcctaccact accagcagaa cacccccatc
1501 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
1561 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
1621 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggctgat ggtgagcaag
1681 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
1741 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
1801 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
1861 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
1921 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caagaatgac
1981 ggcttttaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
2041 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
2101 aacaatcatg accaactgaa aagagaaact aaagtcctga agactctgtc ggtgatcatg
2161 ggtgtgtttg tgtgctgttg gctacctttc ttcatcttga actgcatttt gcccttctgt
2221 gggtctgggg agacgcagcc cttctgcatt gattccaaca cctttgacgt gtttgtgtgg
2281 tttgggtggg ctaattcatc cttgaacccc atcatttatg cctttaatgc tgattttcgg
2341 aaggcatttt caaccctctt aggatgctac agactttgcc ctgcgacgaa taatgccata
2401 gagacggtga gtatcaataa caatggggcc gcgatgtttt ccagccatca tgagccacga
2461 ggctccatct ccaaggagtg caatctggtt tacctgatcc cacatgctgt gggctcctct
2521 gaggacctga aaaaggagga ggcagctggc atcgccagac ccttggagaa gctgtcccca
2581 gccctatcgg tcatattgga ctatgacact gacgtctctc tggagaagat ccaacccatc
2641 acacaaaacg gtcagcaccc aacctgagaa ttcgatatca agcttatcga taatcaacct
2701 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
2761 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
2821 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
2881 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
2941 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
3001 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
3061 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt
3121 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
3181 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
3241 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc
3301 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
3361 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
3421 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
3481 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
3541 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
3601 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
3661 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
3721 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
3781 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
3841 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
3901 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
3961 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4021 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4081 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
4141 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4201 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
4261 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4321 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
4381 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
4441 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
4501 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
4561 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
4621 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
4681 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
4741 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
4861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
4921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
4981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
5101 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
5161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
5221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
5281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
5341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5401 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
5641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
5701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
5761 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5821 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5881 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5941 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6001 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6061 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
6121 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
6181 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
6241 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6301 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6361 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6421 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6481 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6541 ttttgctggc c
//
