| 质粒名称: | pOpen-GBDpol |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165501 |
| 存储实验室: | Drew Endy, Jennifer Molloy, FreeGenes Project |
|
载体骨架基本信息 |
|
| 载体名称: | pBuild |
| 完整质粒大小: | 4439 bp |
| 载体类型: | Synthetic Biology |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Pyrococcus Sp. Heat-Stable DNA Polymerase |
| 物种来源: | Other |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AATG (unknown if destroyed) |
| 3’克隆位点: | GCTT (unknown if destroyed) |
| 5’测序引物 | M13 forward |
| 3’测序引物 | M13 reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pOpen-GBDpol was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165501 ; http://n2t.net/addgene:165501 ; RRID:Addgene_165501) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pOpen-GBDpol 4439 bp ds-DNA circular SYN 26-JUL-2021
DEFINITION Robust and extremely thermostable polymerase with 5x higher fidelity
than Taq. Ideal for GC-rich or looped sequences..
ACCESSION .
VERSION .
KEYWORDS pOpen-GBDpol
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4439)
TITLE Open Enzyme Collection
REFERENCE 2 (bases 1 to 4439)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 26, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4439
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2462..2480)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 2476..2523
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2528..2544)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2528..2544)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin complement(2611..3199)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2691..2710)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3390..4250)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4013..4032
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4251..4355)
/gene="bla"
/label=AmpR promoter
primer_bind 4371..4387
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 4388..4419
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
ORIGIN
1 aaaggggccg tcaatatcag ggtctctaat gatcattgac gctgactaca tcactgaaga
61 tggcaaaccg atcatccgta tttttaaaaa agagaaaggt gaatttaaag tcgaatacga
121 ccgcaccttt cgcccgtaca tttacgcgct gctgaaagat gacagcgcta ttgatgaagt
181 gaagaaaatt actgcggaac gtcatggtaa aattgttcgt attacggagg tggaaaaagt
241 ccaaaaaaaa ttcctgggcc gtcctatcga ggtgtggaag ctgtacctgg aacatcctca
301 ggatgttccg gcaattcgtg aaaaaatccg tgaacacccg gccgtcgtag atatcttcga
361 gtacgatatc ccgttcgcaa aacgttacct gattgataaa ggcctgacgc cgatggaggg
421 caacgaagaa ctgacctttc tggcggtgga catcgaaact ctgtatcacg aaggcgaaga
481 atttggtaaa ggccctatta tcatgatttc gtatgcggat gaggaaggag cgaaagtgat
541 cacatggaaa agcattgacc tgccgtacgt tgaagtggtc agctcagaac gcgaaatgat
601 taaacgcctc gttaaagtca ttcgtgaaaa agatccggac gtaatcatca cctacaatgg
661 tgataacttc gactttccgt atcttctgaa acgtgcggaa aaattgggca ttaaactgcc
721 gcttggtcgt gacaacagcg aaccgaaaat gcagcgcatg ggtgacagcc tggcggtgga
781 aattaaaggt cgtatccact tcgacctgtt cccggcgatc cgtcgtacca tcaacctgcc
841 gacgtatacc ctggaaacgg tttacgaagt tattttcggc aaatccaaag aaaaagttta
901 cgctcacgaa atcgcggaag catgggaaac cggtaaaggc ctggaacgtg tggcaaagta
961 cagcatggaa gatgcaaaag tgaccagcga actggggaaa gaatttttcc caatggaagc
1021 gcaactggca cgcctggtgg gtcatccggt ctgggacgtg tctcgtagct cgactggcaa
1081 cctggtggaa tggttcctgc tgactaaagc ttacgaacgc aacgagctcg caccgaacaa
1141 accggatgaa cgtgaatacg aacgccgtct gcgtgagagc tacgaaggcg gttacgtgaa
1201 cgaaccggaa aaaggcctgt gggagggtat cgtgagcctg gatttccgta gcctgtaccc
1261 gtcgattatc atcactcata acgtatcccc agatacgctc aaccgtgaaa actgtaaaga
1321 atatgacgtt gcgcctcagg tgggtcatcg cttctgcaag gattttccag gtttcatccc
1381 gagcctgctg ggaaacctgc tggaggaacg tcaaaaaatc aaaaaacgca tgaaagagag
1441 taaagacccg gtggaaaaaa agctgctgga ctaccgtcag cgtgctatta agatccttgc
1501 gaactcttat tacggttatt acggctatgc aaaagcgcgt tggtactgca aagaatgcgc
1561 tgaatccgtt actgcatggg gtcgtcagta catcgacctg gtacgtaggg aactggagag
1621 ccgtggcttc aaagtactgt atatcgacac tgatggtctg tacgccacta tcccgggcgc
1681 aaaacatgaa gagatcaaag agaaagccct gaaattcgtc gagtatataa actccaaact
1741 cccgggcctg ctggaactgg aatatgaagg cttctacgcg cgcggcttct ttgtgaccaa
1801 aaaaaaatac gctctgattg atgaggaagg taaaatagtt acccgcggcc tggaaatcgt
1861 gcgccgtgac tggagcgaaa tcgccaaaga gactcaggca aaagttctgg aagcaatcct
1921 gaagcacggc aacgttgacg aggcggtgaa aatcgtaaag gaagttaccg aaaagctgtc
1981 gaagtatgag atcccaccag aaaaactggt tatttacgag caaatcaccc gcccgttatc
2041 agaatacaaa gcaattggcc cgcatgtagc tgtggccaaa cgcctggccg cgaaaggcgt
2101 aaaagttaaa ccgggtatgg taattgggta catcgtcctg aagggagatg gtccaatcag
2161 caaacgtgcc attgctattg aagagtttga tccgaaaaaa cacaaatacg acgccgaata
2221 ctatattgaa aaccaagttc tgccggccgt tgaacgtatc ctgcgcgcgt tcggctaccg
2281 taaggaggat ctgcgctacc aaaaaaccaa acaggtgggt ctgggtgctt ggctgaaatt
2341 ttgaagagct ttgagaccac ggagccctta ttgtaaccgc ttcctacgcg cgctaaacct
2401 agggataaca gggtaatttg tcagtcgatt cagtcggccg ctgaggtgtc aatcgtcgga
2461 gccgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
2521 ttgcatggtc atagctgttt cctgagagct tggcaggtga tgacacacat taacaaattt
2581 cgtgaggagt ctccagaaga atgccattaa tttccatagg ctccgccccc ctgacgagca
2641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
2701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
2761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
3001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
3061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
3121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacacg
3181 cagaaaaaaa ggatctcaag aaggcctact attagcaaca acgatccttt gatcttttct
3241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
3301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
3421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
3481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
3541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
3601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
3661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
3721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
3781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
3841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
3901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
3961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
4021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
4081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
4141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
4201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
4261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
4321 tgtatttaga aaaataaaca aataggggtt ccgcgcacct gcaccagtca gtaaaacgac
4381 ggccagtagt caaaagcctc cgaccggagg cttttgactt ggttcaggtg gagtgggag
//
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