pACYC177

价格:850元

联系方式:I47-825O-882O

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载体基本信息

出品公司: ATCC
载体名称: pACYC177
质粒类型: 大肠杆菌克隆载体
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 3941bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Ampicillin
筛选标记: --
备注: --
产品目录号: 37031
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pACYC177载体图谱
pACYC177载体特征

载体简介

pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.

载体序列

LOCUS       pACYC177	3941 bp 	DNA	SYN
DEFINITION  pACYC177
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="pACYC177"
                     /mol_type="other DNA"
     gene            10..618
                     /label="Ampicillin (252 - 860)"
                     /gene="Ampicillin (252 - 860)"
     CDS             100..618
                     /label="ORF frame 1"
                     /translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVT
                     RLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAG
                     PLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQ
                     IAEIGASLIKHW*"
     rep_origin      complement(767..1595)
                     /label="p15A_origin"
                     /translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVT
                     RLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAG
                     PLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQ
                     IAEIGASLIKHW*"
     gene            1923..2738
                     /label="KanR2"
                     /gene="KanR2"
                     /translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVT
                     RLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAG
                     PLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQ
                     IAEIGASLIKHW*"
     CDS             1923..2738
                     /label="ORF frame 3"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_feature    3025..3515
                     /label="Tn3R"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     promoter        3629..3657
                     /label="AmpR_promoter"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     gene            3699..618
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     CDS             3699..618
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
   61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
  121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
  181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
  241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
  301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
  361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
  421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
  481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
  541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
  601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
  661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
  721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct
  781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
  841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
  901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
  961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
 1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
 1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
 1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
 1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
 1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
 1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
 1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
 1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
 1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
 1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
 1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
 1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
 1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
 1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
 1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
 1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg
 1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
 2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
 2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
 2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
 2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
 2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
 2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
 2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
 2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
 2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
 2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
 2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
 2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
 2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac
 2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc
 2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg
 2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc
 3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt
 3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag
 3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc
 3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac
 3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg
 3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag
 3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt
 3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg
 3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
 3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
 3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
 3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
 3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
 3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
 3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
 3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t
//

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