pFLAG-CMV-2

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: SIGMA
载体名称: pFLAG-CMV-2
质粒类型: 哺乳动物表达载体
高拷贝/低拷贝: 低拷贝
克隆方法: 多克隆位点;限制性内切酶
启动子: CMV
载体大小: 4679 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: hGH-pA-R:CCAGCTTGGTTCCCAATAGA
载体标签: FLAG tag (N-末端)
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: --
宿主细胞(系): 常用细胞系,如293、Hela等
备注: CMV启动子驱动目的蛋白高 水平表达;
FLAG标签含8个氨基酸残基(DYKDDDDK),
一般位于融合蛋白的外表面,具有高度免疫 灵敏性,便于检测和纯化目的蛋白;
产品目录号: E7398
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pFLAG-CMV-2 载体图谱



pFLAG-CMV-2 多克隆位点

载体简介

载体序列

LOCUS       pFLAG-CMV-2	4679 bp 	DNA	SYN
DEFINITION  pFLAG-CMV-2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="pFLAG-CMV-2"
                     /mol_type="other DNA"
     misc_feature    complement(12..155)
                     /label="lacZ_a"
     misc_feature    126..148
                     /label="M13_pUC_fwd_primer"
     promoter        141..157
                     /label="M13_forward20_primer"
     promoter        319..895
                     /label="CMV_immearly_promoter"
     misc_feature    398..685
                     /label="CAG_enhancer"
     promoter        841..912
                     /label="CMV_promoter"
     misc_feature    852..872
                     /label="CMV_fwd_primer"
     misc_feature    896..915
                     /label="pCEP_fwd_primer"
     terminator      1030..1496
                     /label="hGH_PA_terminator"
     misc_feature    complement(1666..1686)
                     /label="pBABE_3_primer"
     misc_feature    complement(1672..1887)
                     /label="SV40_enhancer"
     promoter        1684..1952
                     /label="SV40_promoter"
     rep_origin      1851..1928
                     /label="SV40_origin"
     misc_feature    1913..1932
                     /label="SV40pro_F_primer"
     promoter        complement(2039..2057)
                     /label="T7_promoter"
     misc_feature    complement(2084..2106)
                     /label="M13_pUC_rev_primer"
     promoter        complement(2120..2149)
                     /label="lac_promoter"
     gene            complement(3232..4092)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3232..4092)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4134..4162)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4241..4547
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
   61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
  121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
  181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
  241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
  301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
  361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
  421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
  481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
  541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
  601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
  661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
  721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
  781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
  841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
  901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
  961 tgcggccgcg aattcatcga tagatctgat atcggtacca gtcgactcta gaggatcccg
 1021 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
 1081 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
 1141 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
 1201 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
 1261 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
 1321 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
 1381 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
 1441 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
 1501 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg
 1561 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
 1621 ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt
 1681 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
 1741 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
 1801 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
 1861 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
 1921 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
 1981 gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc
 2041 tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
 2101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
 2161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
 2221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
 2281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
 2341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
 2401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
 2461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
 2521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
 2581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
 2641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
 2701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
 2761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
 2821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
 2881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
 2941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
 3001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
 3061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
 3121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
 3181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
 3241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
 3301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
 3361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
 3421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
 3481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
 3541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
 3601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
 3661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
 3721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
 3781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
 3841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
 3901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
 3961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
 4021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
 4081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
 4141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
 4201 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
 4261 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
 4321 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
 4381 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
 4441 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
 4501 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
 4561 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
 4621 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattt

//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng