p0521s

LOCUS       p0521s               16310 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Episome vector for Phaeodactylum tricornutum (pCC1BAC-based).
ACCESSION   .
VERSION     .
KEYWORDS    p0521s
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16310)
  AUTHORS   Karas BJ, Diner RE, Lefebvre SC, McQuaid J, Phillips AP, Noddings 
            CM, Brunson JK, Valas RE, Deerinck TJ, Jablanovic J, Gillard JT, 
            Beeri K, Ellisman MH, Glass JI, Hutchison CA 3rd, Smith HO, Venter 
            JC, Allen AE, Dupont CL, Weyman PD
  TITLE     Designer diatom episomes delivered by bacterial conjugation.
  JOURNAL   Nat Commun. 2015 Apr 21;6:6925. doi: 10.1038/ncomms7925.
  PUBMED    25897682
REFERENCE   2  (bases 1 to 16310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      6..620
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      696..915
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1006..1761
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1764..2014
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     misc_feature    2176..2679
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        2680..2866
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             2867..3529
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             3719..4894
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4894..5865
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5938..6411
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6671..7069
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    7087..7120
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     7188..7207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(7287..7562)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     primer_bind     7400..7422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7414..7431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7415..7431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7438..7457
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7438..7456
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7459..7515
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7558..7574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7558..7574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7571..7593)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7582..7598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7606..7636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7651..7672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(7892..8551)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8466..8485
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8552..8654)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        9530..9548
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             10252..10626
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        11354..11569
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11570..12373
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     oriT            13131..13240
                     /note="incP origin of transfer"
     CDS             13273..13644
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 tcgagctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca aacgcgccag
       61 aaacgccgtc gaagccgtgt gcgagacacc gcggccggcc gccggcgttg tggatacctc
      121 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
      181 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
      241 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
      301 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
      361 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
      421 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
      481 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
      541 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
      601 cccccttctc gaaccctccc ggtcgagtga gcgaggaagc accagggaac agcacttata
      661 tattctgctt acacacgatg cctgaaaaaa cttcccttgg ggttatccac ttatccacgg
      721 ggatattttt ataattattt tttttatagt ttttagatct tcttttttag agcgccttgt
      781 aggcctttat ccatgctggt tctagagaag gtgttgtgac aaattgccct ttcagtgtga
      841 caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat
      901 tgccctcaga agaagctgtt ttttcacaaa gttatccctg cttattgact cttttttatt
      961 tagtgtgaca atctaaaaac ttgtcacact tcacatggat ctgtcatggc ggaaacagcg
     1021 gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact
     1081 gaggcggcat atagtctctc ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag
     1141 atcagaaaat ctgatggcac cctacaggaa catgacggta tctgcgagat ccatgttgct
     1201 aaatatgctg aaatattcgg attgacctct gcggaagcca gtaaggatat acggcaggca
     1261 ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat
     1321 gaaaaaggct atgaatcttt tccttggttt atcaaacgtg cgcacagtcc atccagaggg
     1381 ctttacagtg tacatatcaa cccatatctc attcccttct ttatcgggtt acagaaccgg
     1441 tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca atccgtatgc catgcgttta
     1501 tacgaatccc tgtgtcagta tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc
     1561 gactggatca tagagcgtta ccagctgcct caaagttacc agcgtatgcc tgacttccgc
     1621 cgccgcttcc tgcaggtctg tgttaatgag atcaacagca gaactccaat gcgcctctca
     1681 tacattgaga aaaagaaagg ccgccagacg actcatatcg tattttcctt ccgcgatatc
     1741 acttccatga cgacaggata gtctgagggt tatctgtcac agatttgagg gtggttcgtc
     1801 acatttgttc tgacctactg agggtaattt gtcacagttt tgctgtttcc ttcagcctgc
     1861 atggattttc tcatactttt tgaactgtaa tttttaagga agccaaattt gagggcagtt
     1921 tgtcacagtt gatttccttc tctttccctt cgtcatgtga cctgatatcg ggggttagtt
     1981 cgtcatcatt gatgagggtt gattatcaca gtttattact ctgaattggc tatccgcgtg
     2041 tgtacctcta cctggagttt ttcccacggt ggatatttct tcttgcgctg agcgtaagag
     2101 ctatctgaca gaacagttct tctttgcttc ctcgccagtt cgctcgctat gctcggttac
     2161 acggctgcgg cgagcatcac gtgctataaa aataattata atttaaattt tttaatataa
     2221 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     2281 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta ccttgctctt
     2341 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     2401 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     2461 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     2521 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
     2581 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
     2641 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc tagtacactc tatatttttt
     2701 tatgcctcgg taatgatttt catttttttt ttccacctag cggatgactc tttttttttc
     2761 ttagcgattg gcattatcac ataatgaatt atacattata taaagtaatg tgatttcttc
     2821 gaagaatata ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga cagagcagaa
     2881 agccctagta aagcgtatta caaatgaaac caagattcag attgcgatct ctttaaaggg
     2941 tggtccccta gcgatagagc actcgatctt cccagaaaaa gaggcagaag cagtagcaga
     3001 acaggccaca caatcgcaag tgattaacgt ccacacaggt atagggtttc tggaccatat
     3061 gatacatgct ctggccaagc attccggctg gtcgctaatc gttgagtgca ttggtgactt
     3121 acacatagac gaccatcaca ccactgaaga ctgcgggatt gctctcggtc aagcttttaa
     3181 agaggcccta ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg cgcctttgga
     3241 tgaggcactt tccagagcgg tggtagatct ttcgaacagg ccgtacgcag ttgtcgaact
     3301 tggtttgcaa agggagaaag taggagatct ctcttgcgag atgatcccgc attttcttga
     3361 aagctttgca gaggctagca gaattaccct ccacgttgat tgtctgcgag gcaagaatga
     3421 tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt gccataagag aagccacctc
     3481 gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagt tttacacagg
     3541 agtctggact tgacgctagt gataataagt gactgaggta tgtgctcttc ttatctcctt
     3601 ttgtagtgtt gctcttattt taaacaactt tgcggttttt tgatgacttt gcgattttgt
     3661 tgttgctttg cagtaaattg caagatttaa taaaaaaacg caaagcaatg attaaaggat
     3721 gttcagaatg aaactcatgg aaacacttaa ccagtgcata aacgctggtc atgaaatgac
     3781 gaaggctatc gccattgcac agtttaatga tgacagcccg gaagcgagga aaataacccg
     3841 gcgctggaga ataggtgaag cagcggattt agttggggtt tcttctcagg ctatcagaga
     3901 tgccgagaaa gcagggcgac taccgcaccc ggatatggaa attcgaggac gggttgagca
     3961 acgtgttggt tatacaattg aacaaattaa tcatatgcgt gatgtgtttg gtacgcgatt
     4021 gcgacgtgct gaagacgtat ttccaccggt gatcggggtt gctgcccata aaggtggcgt
     4081 ttacaaaacc tcagtttctg ttcatcttgc tcaggatctg gctctgaagg ggctacgtgt
     4141 tttgctcgtg gaaggtaacg acccccaggg aacagcctca atgtatcacg gatgggtacc
     4201 agatcttcat attcatgcag aagacactct cctgcctttc tatcttgggg aaaaggacga
     4261 tgtcacttat gcaataaagc ccacttgctg gccggggctt gacattattc cttcctgtct
     4321 ggctctgcac cgtattgaaa ctgagttaat gggcaaattt gatgaaggta aactgcccac
     4381 cgatccacac ctgatgctcc gactggccat tgaaactgtt gctcatgact atgatgtcat
     4441 agttattgac agcgcgccta acctgggtat cggcacgatt aatgtcgtat gtgctgctga
     4501 tgtgctgatt gttcccacgc ctgctgagtt gtttgactac acctccgcac tgcagttttt
     4561 cgatatgctt cgtgatctgc tcaagaacgt tgatcttaaa gggttcgagc ctgatgtacg
     4621 tattttgctt accaaataca gcaatagcaa tggctctcag tccccgtgga tggaggagca
     4681 aattcgggat gcctggggaa gcatggttct aaaaaatgtt gtacgtgaaa cggatgaagt
     4741 tggtaaaggt cagatccgga tgagaactgt ttttgaacag gccattgatc aacgctcttc
     4801 aactggtgcc tggagaaatg ctctttctat ttgggaacct gtctgcaatg aaattttcga
     4861 tcgtctgatt aaaccacgct gggagattag ataatgaagc gtgcgcctgt tattccaaaa
     4921 catacgctca atactcaacc ggttgaagat acttcgttat cgacaccagc tgccccgatg
     4981 gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat tactttgcct
     5041 gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag tgttgagaag
     5101 acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga ggacgcactg
     5161 gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt cggtcgaaga
     5221 gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc tgcacttacc
     5281 gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc tgcattatcc
     5341 agattgggta acgattatcg cccaacaagt gcttatgaac gtggtcagcg ttatgcaagc
     5401 cgattgcaga atgaatttgc tggaaatatt tctgcgctgg ctgatgcgga aaatatttca
     5461 cgtaagatta ttacccgctg tatcaacacc gccaaattgc ctaaatcagt tgttgctctt
     5521 ttttctcacc ccggtgaact atctgcccgg tcaggtgatg cacttcaaaa agcctttaca
     5581 gataaagagg aattacttaa gcagcaggca tctaaccttc atgagcagaa aaaagctggg
     5641 gtgatatttg aagctgaaga agttatcact cttttaactt ctgtgcttaa aacgtcatct
     5701 gcatcaagaa ctagtttaag ctcacgacat cagtttgctc ctggagcgac agtattgtat
     5761 aagggcgata aaatggtgct taacctggac aggtctcgtg ttccaactga gtgtatagag
     5821 aaaattgagg ccattcttaa ggaacttgaa aagccagcac cctgatgcga cctcgtttta
     5881 gtctacgttt atctgtcttt acttaatgtc ctttgttaca ggccagaaag cataactggc
     5941 ctgaatattc tctctgggcc cactgttcca cttgtatcgt cggtctgata atcagactgg
     6001 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg
     6061 tatcgtcggt ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgataa
     6121 tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccatgg
     6181 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
     6241 gtctgattat tagtctggaa ccacggtccc actcgtatcg tcggtctgat tattagtctg
     6301 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac gatcccactc
     6361 gtgttgtcgg tctgattatc ggtctgggac cacggtccca cttgtattgt cgatcagact
     6421 atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg caagtattga catgtcgtcg
     6481 taacctgtag aacggagtaa cctcggtgtg cggttgtatg cctgctgtgg attgctgctg
     6541 tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg acaaaatacc tggttaccca
     6601 ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag tgtgtcgctg tcgacgagct
     6661 cgcgagctcg gacatgaggt tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt
     6721 tgtttttacg ttaagttgat gcagatcaat taatacgata cctgcgtcat aattgattat
     6781 ttgacgtggt ttgatggcct ccacgcacgt tgtgatatgt agatgataat cattatcact
     6841 ttacgggtcc tttccggtga tccgacaggt tacggggcgg cgacctcgcg ggttttcgct
     6901 atttatgaaa attttccggt ttaaggcgtt tccgttcttc ttcgtcataa cttaatgttt
     6961 ttatttaaaa taccctctga aaagaaagga aacgacaggt gctgaaagcg agctttttgg
     7021 cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag tccgagctca
     7081 tcgctaataa cttcgtatag catacattat acgaagttat attcgatgcg gccgcaaggg
     7141 gttcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     7201 gattgtactg agagtgcacc atatgcggtg tgaaatacca cacagatgcg taaggagaaa
     7261 ataccgcatc aggcgccatt cgccattcag ctgcgcaact gttgggaagg gcgatcggtg
     7321 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     7381 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     7441 tacgactcac tatagggcga attcgagctc ggtacccggg gatcctctag agtcgacctg
     7501 caggcatgca agcttgagta ttctatagtc tcacctaaat agcttggcgt aatcatggtc
     7561 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7621 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7681 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7741 ccaacgcgaa ccccttgcgg ccgcccgggc cgtcgaccaa ttctcatgtt tgacagctta
     7801 tcatcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg
     7861 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt
     7921 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     7981 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     8041 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     8101 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     8161 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     8221 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     8281 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     8341 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     8401 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     8461 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     8521 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     8581 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     8641 ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa
     8701 cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgc
     8761 gataagctca tggagcggcg taaccgtcgc acaggaagga cagagaaagc gcggatctgg
     8821 gaagtgacgg acagaacggt caggacctgg attggggagg cggttgccgc cgctgctgct
     8881 gacggtgtga cgttctctgt tccggtcaca ccacatacgt tccgccattc ctatgcgatg
     8941 cacatgctgt atgccggtat accgctgaaa gttctgcaaa gcctgatggg acataagtcc
     9001 atcagttcaa cggaagtcta cacgaaggtt tttgcgctgg atgtggctgc ccggcaccgg
     9061 gtgcagtttg cgatgccgga gtctgatgcg gttgcgatgc tgaaacaatt atcctgagaa
     9121 taaatgcctt ggcctttata tggaaatgtg gaactgagtg gatatgctgt ttttgtctgt
     9181 taaacagaga agctggctgt tatccactga gaagcgaacg aaacagtcgg gaaaatctcc
     9241 cattatcgta gagatccgca ttattaatct caggagcctg tgtagcgttt ataggaagta
     9301 gtgttctgtc atgatgcctg caagcggtaa cgaaaacgat ttgaatatgc cttcaggaac
     9361 aatagaaatc ttcgtgcggt gttacgttga agtggagcgg attatgtcag caatggacag
     9421 aacaacctaa tgaacacaga accatgatgt ggtctgtcct tttacagcca gtagtgctcg
     9481 ccgcagtcga gcgacagggc gaagcccacc atgattacgc caagctcgaa attaaccctc
     9541 actaaaggga acaaaagctg gtacctaaca ggattagtgc aattcgagtt gaatcactgg
     9601 gaaaaacatt gtcttctttt ttatattatc atttgcatta gtgctgcagt cgtagatact
     9661 tgttggttga aagacatcag ctgggaggga ctggactagc gtttggtaag gagacatacc
     9721 tgttaacgtt ggttgcaaaa ttccatttcg cgatttatgt tatctgtaaa tcctgatttg
     9781 tctggaattc ttgatacttc cgttttttta gaggccaatg attagcatcg gcgattctca
     9841 aaatagcatt ttcgacatgc ggtgctgatt tcataaacat agacaacgct tttacatgta
     9901 aaagtaactt gcggacttgg aacagtgctc tgtttttggt gtgaacgtaa ctcagcaata
     9961 tttctgtgct agcaaggttt tttatgatcg accgaagatc tcaaaactcc gggtctttca
    10021 actgtctgac tagaccatgt tcgtaacgtc ggacagcagc tttcgttgta ctggtagaat
    10081 ttctacgtgc gaagcacgtg taggcaggtt gaacgacgat ccctgccgat ggatggattg
    10141 gcacgcggcg gaacgctttc gtgatctaca ccacctggat cttcacatat cttcgaaatc
    10201 gaaaaattaa ccaagtcgac ggtatcgata atattctagc tgagggtacc catggccaag
    10261 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
    10321 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
    10381 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
    10441 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
    10501 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
    10561 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
    10621 gactgaccga cgccgaccaa caccgccggt ccgacgcggc ccgacgggtc cgaggcctcg
    10681 gagatctggg cccatgcggc cgcaacaact acctcgactt tggctgggac actttcagtg
    10741 aggacaagaa gcttcagaag cgtgctatcg aactcaacca gggacgtgcg gcacaaatgg
    10801 gcatccttgc tctcatggtg cacgaacagt tgggagtctc tatccttcct taaaaattta
    10861 attttcatta gttgcagtca ctccgctttg gtttcacagt caggaataac actagctcgt
    10921 cttcacgtaa ctataacggt cctaaggtag cgaacgttgc aggccatgct gtccaggcag
    10981 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
    11041 tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
    11101 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
    11161 ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat
    11221 tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca
    11281 acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatcg
    11341 gggggggggg ggtttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
    11401 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
    11461 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
    11521 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
    11581 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
    11641 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
    11701 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
    11761 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
    11821 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
    11881 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
    11941 ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg gaagaagtaa caaaggaacc
    12001 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctagcta ctggagaata
    12061 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
    12121 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
    12181 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
    12241 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
    12301 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
    12361 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
    12421 agcttcaatt taattatatc agttattacc cggccgggaa tctcggtcgt aatgattttt
    12481 ataatgacga aaaaaaaaaa aattggaaag aaaacccccc cccccccgca gcgttgggtc
    12541 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
    12601 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
    12661 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
    12721 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
    12781 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
    12841 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
    12901 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
    12961 catcgagtta cgctagggat aacaggtaat atagcatctt ccgctgcata accctgcttc
    13021 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
    13081 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
    13141 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
    13201 gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat
    13261 gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag
    13321 gtgtactgcc ttccagacga acgaagagcg attgaggaaa aggcggcggc ggccggcatg
    13381 agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac
    13441 tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg
    13501 ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc
    13561 gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg
    13621 gtccgcccga gggcagagcc atgacttttt tagccgctaa aacggccggg gggtgcgcgt
    13681 gattgccaag cacgtcccca tgcgctccat caagaagagg cacttcgagc tgtaagtaca
    13741 tcaccgacga gcaaggcaag acgatccgcg cctgttttat tgagaacgtt gttcgtgttg
    13801 gcctcaatgg tagcgatgcg tcattcagcg aagttctggt gctgatgatg tggttcgctt
    13861 tgccactggt caatgtggta agcccgtgta atgtcagtaa cctttttact gatctcagct
    13921 tgagcacggt cgctgatgag cttatccatg gccccacggt aacggatatg atcctctagg
    13981 gcgttgacaa attctttgtc ggttttcatg gggtagacgt cagtgaccag ggaacgtctc
    14041 cctacaaaaa gttgcgcata ctttgctccg ttgtcgacgg caggggtgtc ggaccagatt
    14101 gtatctgtgg caacggcctc attgcgtcaa tggaccttta aagccgggaa acgagacttg
    14161 aaatgtttac gtagaggtgc attatagacc tcacgcgcat attgagtggt tgcaaaaatg
    14221 gttttgcgaa cggtatcact ggagacccat gccaggcaag gacggagggc atcataatca
    14281 tgttcattgc gttggacagc atgcttgtta cacgtaagta tacggtcgag agcatcgtga
    14341 cgttgagaat tacagatggc aacgtgtcgg tgcatgtatt ggccgagatt gtcaaatcgt
    14401 ggctcaatat atggcatgcc aggtaagtca tggatgtcgg tatgccattc agccttcatg
    14461 tcgatttcgt tgtcgagaac tgatgggtcc cagtcaacgt cagaagtaag aacaacatgg
    14521 ggaagggaat gaagttcatg actggtgggt gcgcgcatat ccatgtacgg aagaccctgt
    14581 cgtatgttta gtgggatgat gtaatcatcc agggtaacaa tacgttggtt gccgcccacg
    14641 gtacgagacc gatcttgaac acaattgtgg caatgttcaa gttgggcgct ggaatggatg
    14701 gttttacctt taccaagatg cgcgtattga tgcatgataa caacaatggg ccctcgttgc
    14761 gattcgacaa gaccggcagc cgtgacaatg tctagattgg acaaggtatg ttcatttata
    14821 ccggttatgt tggctgaacg tccagttttg tgcagtacgg taacatcact ccccgcaagt
    14881 ccaccgttgg caccgcgatc gacaagtgca gatgtggtat tttgtactgt gtgtcgggag
    14941 acttggtact gtaacacatt tgactgaagt gacttggtgg taccatgagg acggagggaa
    15001 cccgttgata cggtgttgcg tgacgctgca agtaccttac gtatgtcgcc tggatccata
    15061 cgtccaaccc gatcagaaag atgtgctagg agttccgttt ctggtgcaca atcatgaaag
    15121 gtatccacgg gagttccatt gttgtcgtcc ggggctgcgt cgcgactgcc agttgcatgt
    15181 acatgcgcca tcaagggacg tgccaatcca ttactaaccg ggagaacctt agctggggca
    15241 gccaatccct gaagtatagc cttagcatca tctgagagct ggttccacat atctctaggg
    15301 atataaggtc gttcacggtt agtgggcgta ctatgatgtc ggttattagt acaattctcc
    15361 ctgcgggcat gcgcattggc ttcatagagt acggaaggtg acaagtcaat attgtagtta
    15421 acatccggat cagtgtcaaa gtctgtagga tggtaagaaa gatcagtaga atgaatacta
    15481 cgcttttcct tgggactacg ggaattagag aagttgtttc ctttattgta gagtgacgct
    15541 gaagcaagta gaagactaag gtaactctcg tagctaatag gattacctcc tttggctagg
    15601 tcaagagtgg ctgtgatctt cacttgacaa agttccggta cattgtggac agcattctcc
    15661 aaaagactaa gacacagttg ctttgggagt tgctcagcca ttggtacagt attgtggtag
    15721 atacaaaggt ggttcttcca atgaaggata aatccttctg ctgtgcctgt ccatgaggat
    15781 ccatatttcg ccgtagttag gtaaccaagc gtggtggctg aactgatctt cgcacttgct
    15841 gattccgtat agtgtttgac aactttacaa aacacttctt gcgcagttcg ctctagcctg
    15901 gacagagaag ggacaatctt cgtcgttaag actcgtgcga atagcaaaag atcacaaaat
    15961 agcacatcgg caccgaccaa cgattatttc caaggaaaaa aagaatgctt cactacaaga
    16021 aattgtgtca tccctataca gagtcttgtt actgtgacag aaaattgatg gaagatgtgg
    16081 cggattgcct ttacactagc caacttgttc gactaattgc agcttcttct gagaggcttc
    16141 accgagtaac gcgaagaaca ccggtgtctc gtacatgctc gtcggtgaac gctcgtccaa
    16201 tgacaccccc cactttgtat caatatccca acttggtagt gaactggaat gatacatgca
    16261 atttcgcgcc gcatcaacag ccacgggcca ccatcgacga atagactcgg
//