pESC-His

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Agilent 安捷伦
载体名称: pESC-His, pESC His
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1和GAL10
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6706 bp
5' 测序引物及序列: GAL1-F: AATATACCTCTATACTTTAACGTC
3' 测序引物及序列: CYC1-R: GCGTGAATGTAAGCGTGAC
载体标签: C-Flag, C-Myc
载体抗性: 氨苄
筛选标记: His3
备注: 利用半乳糖诱导,可以同时使两个基因在酿酒酵母中表达。
产品目录号: 217451
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pESC-His载体图谱

pESC-His多克隆位点

载体简介

载体序列

  
LOCUS       pESC-HIS                6706 bp    DNA     circular SYN 16-SEP-2005
DEFINITION  Cloning vector pESC-HIS, complete sequence.
ACCESSION   AF063850
VERSION     AF063850.1  GI:3228560
SOURCE      Cloning vector pESC-HIS.
  ORGANISM  Cloning vector pESC-HIS
            artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Cloning vector pESC-HIS"
                     /mol_type="genomic DNA"
                     /specific_host="Escherichia coli K12"
                     /db_xref="taxon:76667"
                     /vntifkey="98"
     promoter        2556..3010
                     /vntifkey="29"
                     /label=GAL1\Promoter
     promoter        complement(2344..2555)
                     /vntifkey="29"
                     /label=GAL10\promoter
     CDS             504..1160
                     /vntifkey="4"
                     /label=HIS3
                     /note="yeast HIS3 ORF"
     rep_origin      complement(1555..1861)
                     /vntifkey="33"
                     /label=f1\ori
     terminator      complement(1961..2125)
                     /vntifkey="43"
                     /label=ADH1\terminator
     misc_feature    complement(2256..2339)
                     /vntifkey="21"
                     /label=MCS1
     misc_feature    complement(2274..2300)
                     /vntifkey="21"
                     /label=FLAG\tag
     misc_feature    3012..3087
                     /vntifkey="21"
                     /label=MCS2
     misc_feature    3052..3087
                     /vntifkey="21"
                     /label=c-myc\tag
     terminator      3114..3303
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      3490..4157
                     /vntifkey="33"
                     /label=pUC\ori
     CDS             complement(4308..5165)
                     /vntifkey="4"
                     /label=AmpR
     rep_origin      5299..6454
                     /vntifkey="33"
                     /label=2\micron\ori
BASE COUNT     1868 a      1467 c      1480 g      1891 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc 
     1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa 
     2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata 
     2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg 
     2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg 
     2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg 
     2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg 
     2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt 
     2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat 
     2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt 
     2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg 
     2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt 
     2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact 
     2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat 
     2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct 
     2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa 
     2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca 
     2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga 
     3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag 
     3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct 
     3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 
     3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga 
     3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 
     3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 
     5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 
     5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 
     5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa 
     5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa 
     5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt 
     5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 
     5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg 
     5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat 
     5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg 
     5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt 
     5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 
     6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt 
     6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag 
     6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta 
     6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt 
     6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag 
     6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat 
     6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga 
     6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta 
     6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat 
     6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt 
     6601 ctcatccttc aatgctatca tttcctttga tattggatca tctaagaaac cattattatc 
     6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc 
//

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