| 出品公司: | Agilent 安捷伦 |
|---|---|
| 载体名称: | pESC-His, pESC His |
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1和GAL10 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6706 bp |
| 5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC |
| 3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
| 载体标签: | C-Flag, C-Myc |
| 载体抗性: | 氨苄 |
| 筛选标记: | His3 |
| 备注: | 利用半乳糖诱导，可以同时使两个基因在酿酒酵母中表达。 |
| 产品目录号: | 217451 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pESC-HIS 6706 bp DNA circular SYN 16-SEP-2005
DEFINITION Cloning vector pESC-HIS, complete sequence.
ACCESSION AF063850
VERSION AF063850.1 GI:3228560
SOURCE Cloning vector pESC-HIS.
ORGANISM Cloning vector pESC-HIS
artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6706
/organism="Cloning vector pESC-HIS"
/mol_type="genomic DNA"
/specific_host="Escherichia coli K12"
/db_xref="taxon:76667"
/vntifkey="98"
promoter 2556..3010
/vntifkey="29"
/label=GAL1\Promoter
promoter complement(2344..2555)
/vntifkey="29"
/label=GAL10\promoter
CDS 504..1160
/vntifkey="4"
/label=HIS3
/note="yeast HIS3 ORF"
rep_origin complement(1555..1861)
/vntifkey="33"
/label=f1\ori
terminator complement(1961..2125)
/vntifkey="43"
/label=ADH1\terminator
misc_feature complement(2256..2339)
/vntifkey="21"
/label=MCS1
misc_feature complement(2274..2300)
/vntifkey="21"
/label=FLAG\tag
misc_feature 3012..3087
/vntifkey="21"
/label=MCS2
misc_feature 3052..3087
/vntifkey="21"
/label=c-myc\tag
terminator 3114..3303
/vntifkey="43"
/label=CYC1\terminator
rep_origin 3490..4157
/vntifkey="33"
/label=pUC\ori
CDS complement(4308..5165)
/vntifkey="4"
/label=AmpR
rep_origin 5299..6454
/vntifkey="33"
/label=2\micron\ori
BASE COUNT 1868 a 1467 c 1480 g 1891 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc
1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa
2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata
2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg
2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg
2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg
2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg
2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt
2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat
2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt
2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg
2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt
2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact
2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat
2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct
2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa
2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca
2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga
3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag
3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct
3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
6601 ctcatccttc aatgctatca tttcctttga tattggatca tctaagaaac cattattatc
6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
