pcDNA5 FRT/TO SF-HERC2 (ShB-R)

LOCUS       pcDNA5 FRT/TO SF-HERC2 (ShB-R)               19695 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP
            tagged wild-type HERC2 in Flp-In TREx cells .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA5 FRT/TO SF-HERC2 (ShB-R)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19695)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 19695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..19695
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             991..1056
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1066..1089
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1096..1116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(15647..15664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15653..15877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(16010..16029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    16161..16208
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             16216..17238
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    17368..17489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(17405..17424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     17459..17478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(17538..17554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17538..17554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(17551..17573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    17562..17578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(17586..17616)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17631..17652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(17769..17786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17940..18528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18020..18039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(18699..19559)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19322..19341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19560..19664)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatac tccagcctcc
      961 ggactctagc gctagcacgc gtccaccatg gactacaaag accatgacgg tgattataaa
     1021 gatcatgaca tcgattacaa ggatgacgat gacaaggggt cggcctggag ccacccgcag
     1081 ttcgaaaaag gatccgagaa cctctacttc caaggctcga gtatgccctc tgaatctttc
     1141 tgtttggctg cccaggctcg cctcgactcc aaatggttga aaacagatat acagcttgca
     1201 ttcacaagag atgggctctg tggtctgtgg aatgaaatgg ttaaagatgg agaaattgta
     1261 tacactggaa cagaatcaac ccagaacgga gagctccctc ctagaaaaga tgatagtgtc
     1321 gaaccaagtg gaacaaagaa agaagatctg aatgacaaag agaaaaaaga tgaagaagaa
     1381 actcctgcac ctatatatag ggccaagtca attctggaca gctgggtatg gggcaagcaa
     1441 ccagatgtga atgaactgaa ggagtgtctt tctgtgctgg ttaaagagca gcaggccctg
     1501 gccgtccagt cagccaccac caccctctca gccctgcgac tcaagcagag gctggtgatc
     1561 ttggagcgct atttcattgc cttgaataga accgtttttc aggagaatgt caaagttaag
     1621 tggaaaagca gcggtatttc tctgcctcct gtggacaaaa aaagttcccg gcctgcgggc
     1681 aaaggtgtgg aggggctcgc cagagtggga tcccgagcgg cgctgtcttt tgcctttgcc
     1741 ttcctgcgca gggcctggcg atcaggcgag gatgcggacc tctgcagtga gctgttgcag
     1801 gagtccctgg acgccctgcg agcacttccc gaggcctcgc tctttgacga gagcaccgtg
     1861 tcctctgtgt ggctggaggt ggtggagaga gcgaccaggt tcctcaggtc cgtcgtgacg
     1921 ggggatgttc acggaacgcc agccaccaaa gggccaggaa gcatccccct gcaggaccag
     1981 cacttggccc tggccatcct gctggagctg gctgtgcaga gaggcacgct gagccaaatg
     2041 ttgtctgcca tcctgttgtt gcttcagctg tgggacagcg gggcacagga gactgacaat
     2101 gagcgttccg cccagggcac cagcgcccca cttttgccct tgctgcaaag gttccagagc
     2161 atcatttgca ggaaggatgc accccactcc gagggcgaca tgcacctttt gtctggccct
     2221 ctgagcccca atgagagttt cctgaggtac ctcacccttc cacaagacaa cgagcttgcc
     2281 attgatctgc gacaaacggc ggttgttgtc atggcccatt tagaccgtct ggctacgccc
     2341 tgtatgcctc cgctgtgtag ctctccgaca tctcataagg gatcattgca agaggtcata
     2401 ggttgggggt taataggatg gaaatactat gccaatgtga ttggtccaat ccagtgcgaa
     2461 ggcctggcca acctgggagt cacacagatt gcctgtgcag agaagcgttt cctgattctg
     2521 tcacgcaatg gccgcgtgta cacacaggcc tataatagtg acacgctggc cccacagctg
     2581 gtccaaggcc ttgcctccag aaacattgta aaaattgctg cccattctga tggtcaccac
     2641 tacctagcct tggctgctac tggagaggtg tactcctggg gctgtgggga cggcggacgg
     2701 ctgggccatg gggacactgt gcctttggag gagcctaagg tgatctccgc cttctctgga
     2761 aagcaggccg ggaagcacgt ggtgcacatc gcttgcggga gcacttacag tgcggccatc
     2821 actgccgagg gggagctgta cacctggggc cgcgggaact acggccggct gggccatggc
     2881 tccagtgagg acgaggccat tccgatgctg gtagccgggc ttaaaggact gaaggtcatc
     2941 gatgtggcgt gtgggagtgg ggatgctcaa accctggctg tcactgagaa cgggcaagtg
     3001 tggtcttggg gagatggtga ctatgggaaa ttgggcagag gtggtagtga tggctgcaaa
     3061 accccaaagc tgattgaaaa gcttcaagac ttggatgtgg tcaaagtccg ctgtggaagt
     3121 cagttttcca ttgctttgac gaaagatggc caagtttatt catggggaaa aggtgacaac
     3181 cagagacttg gacatggaac agaggaacat gttcgttatc caaaactctt agaaggcttg
     3241 caagggaaga aggtgattga tgtggctgca ggctccaccc actgcctggc tctgactgag
     3301 gacagcgagg tccacagctg ggggagcaac gaccagtgcc agcactttga caccttgcgc
     3361 gtgaccaagc cagaacctgc agcattgcca ggactggaca ccaaacacat agtgggaatt
     3421 gcctgtgggc ctgcccagag ctttgcttgg tcatcatgtt ctgagtggtc cattggcctc
     3481 cgtgtccctt ttgtggtgga catctgctca atgacttttg agcagctgga tctcctgctt
     3541 cggcaggtga gtgaggggat ggatggttcc gcggactggc ccccgcccca ggagaaagag
     3601 tgtgtggccg tggcaacgct gaatcttcta cgacttcagt tgcatgctgc cattagtcac
     3661 caggttgacc cggaattcct tggtttaggt ctgggcagca tcctcctgaa cagcctgaag
     3721 cagacggtgg tgaccctggc cagcagtgcg ggcgtgctga gcaccgtgca gtcggccgcc
     3781 caggccgtgc tgcagagtgg ctggtccgtg ctgctgccca ccgcggagga gcgggcccgg
     3841 gcactctctg ctctcctgcc ctgcgcagtt tcaggcaatg aagtgaacat aagtccaggt
     3901 cgtcgattca tgattgatct tctggtgggc agcttgatgg ctgatggagg gttggagtca
     3961 gccttacacg cagccattac tgcagagatc caggatattg aagccaaaaa agaagcacag
     4021 aaggaaaaag aaattgatga acaggaagcg aatgcctcaa catttcatag aagcaggact
     4081 ccactggata aagaccttat taatacgggg atctgtgagt cttctggcaa acagtgtttg
     4141 cctctggttc agctcataca acagcttctt agaaacattg cttctcagac tgtagccaga
     4201 ttgaaagatg ttgcccgtcg gatttcatca tgtctggact ttgagcaaca cagtcgtgaa
     4261 agatctgctt cattggattt gttactgcgt tttcaacgtt tgcttattag taaactttat
     4321 ccaggagaaa gtattggtca gacctcagat atttctagtc cagagctaat gggtgttggt
     4381 tccttgctga agaagtacac agccctcctg tgcacgcata tcggggatat actgcctgtg
     4441 gccgccagca ttgcttctac cagctggcgg cacttcgcgg aggtggctta cattgtggaa
     4501 ggggacttta ctggtgttct ccttccagaa ctagtagttt ctatagtgct tctgctcagt
     4561 aaaaatgctg gtctcatgca agaggctgga gctgtacctc tgctgggtgg cctgttggaa
     4621 catctggatc ggttcaacca tctggcacca ggaaaggaac gggatgatca tgaagagtta
     4681 gcctggcctg gcataatgga gtcatttttt acaggtcaga actgtagaaa taatgaggaa
     4741 gtgacactta tacgcaaagc tgatttggag aaccataata aagatggagg cttctggact
     4801 gtgattgacg ggaaggtgta tgatataaag gacttccaga cacagtcgtt aacaggaaat
     4861 agtattcttg ctcagtttgc aggggaagac ccagtggtag ctttggaagc tgctttgcag
     4921 tttgaagaca cccgggaatc catgcacgcg ttttgtgttg gccagtattt ggagcctgac
     4981 caagaaatcg tcaccatacc agatctgggg agtctctctt cacctctgat agacacagag
     5041 aggaatctgg gcctgcttct cggattacac gcttcgtatt tggcaatgag cacaccgctg
     5101 tctcctgtcg agattgaatg tgccaaatgg cttcagtcat ccatcttctc tggaggcctg
     5161 cagaccagcc agatccacta cagctacaac gaggagaaag acgaggacca ctgcagctcc
     5221 ccagggggca cacctgccag caaatctcga ctctgctccc acagacgggc cctgggggac
     5281 cattcccagg catttctgca agccattgca gacaacaaca ttcaggatca caacgtgaag
     5341 gactttttgt gtcaaataga aaggtactgt aggcagtgcc atttgaccac accgatcatg
     5401 tttccccccg agcatcccgt ggaagaggtc ggtcgcttgt tgttatgttg cctcttaaaa
     5461 catgaagatt taggtcatgt ggcattatct ttagttcatg caggtgcact tggtattgag
     5521 caagtaaagc acagaacgtt gcctaagtca gtggtggatg tttgtagagt tgtctaccaa
     5581 gcaaaatgtt cgctcattaa gactcatcaa gaacagggcc gttcttacaa ggaggtctgc
     5641 gctcctgtca tcgaacgttt gagattcctc tttaatgaat tgagacctgc tgtttgtaat
     5701 gacctctcta taatgtctaa gtttaaattg ttaagttctt tgccccgttg gaggaggata
     5761 gctcaaaaga taattcgaga acgaaggaaa aagagagttc ctaagaagcc agaatctacg
     5821 gatgatgaag aaaaaattgg aaacgaagag agtgatttag aagaagcttg cattttgcct
     5881 catagtccaa taaatgtgga caagagaccc attgcaatta aatcacccaa ggacaaatgg
     5941 cagccgctgt tgagtactgt tacaggtgtt cacaaataca agtggttgaa gcagaatgtg
     6001 cagggtcttt atccgcagtc tccactcctc agtacaattg ctgaatttgc ccttaaagaa
     6061 gagccagtgg atgtggaaaa aatgagaaag tgcctactaa aacagttgga gagagcagag
     6121 gttcgcctgg aagggataga tacaatttta aaactggcga gcaagaattt cttacttcca
     6181 tctgtgcagt atgcgatgtt ttgtggatgg caaagactta ttcctgaggg aatcgatata
     6241 ggggaacctc ttactgattg tttaaaggat gttgatttga tcccgccttt taatcggatg
     6301 ctgctggaag tcacctttgg caagctgtac gcttgggctg tacagaacat tcgaaatgtt
     6361 ttgatggatg ccagtgccaa atttaaagag cttggtatcc agccggttcc cctgcaaacc
     6421 atcaccaatg agaacccgtc aggaccgagc ctggggacca tcccgcaagc ccgcttcctc
     6481 ctggtgatgc tcagcatgct caccctgcag cacggcgcaa acaacctcga ccttctgctc
     6541 aattccggca tgctggccct cacgcagacg gcactgcgcc tgattggccc cagttgtgac
     6601 aacgttgagg aagatatgaa tgcttctgct caaggtgctt ctgccacagt tttggaagaa
     6661 acaaggaagg aaacggctcc tgtgcagctc cctgtttcag gaccagaact ggctgccatg
     6721 atgaagattg gaacaagggt catgagaggt gtggactgga aatggggcga tcaggatggg
     6781 cctcctccag gcctaggccg cgtgattggt gagctgggag aggacggatg gataagagtc
     6841 cagtgggaca caggcagcac caactcctac aggatgggga aagaaggaaa atacgacctc
     6901 aagctggcag agctgccggc tgctgcacag ccctcagcag aggattcgga cacagaggat
     6961 gactctgaag ccgaacaaac tgaaaggaac attcacccca ctgcaatgat gtttaccagc
     7021 actattaact tactgcagac tctttgtctg tctgctggag ttcatgctga gatcatgcag
     7081 agcgaagcca ccaagacttt atgcggactg ctgcgaatgt tagtggaaag cggaacgacg
     7141 gacaagacat cttctccaaa caggctggtg tacagggagc aacaccggag ctggtgcacg
     7201 ctggggtttg tgcggagcat cgctctcacg ccgcaggtat gcggcgccct cagctccccg
     7261 cagtggatca cgctgctcat gaaggtcgtg gaagggcacg cacccttcac tgccacctcg
     7321 ctgcagaggc agatcttagc tgtgcatttg ttgcaagcag tccttccatc atgggacaag
     7381 accgaaaggg cgagggacat gaaatgcctc gtggagaagc tgtttgactt cttgggaagc
     7441 ttgctcacta cctgctcctc tgacgtgcca ttactcagag agtccacgct gaggcggcgc
     7501 agggtgcgcc cgcaggcctc gctgactgcc acccacagca gcacactggc ggaggaggtg
     7561 gtggcactgc tgcgcacgct gcactccctg actcagtgga atgggctcat caacaagtac
     7621 atcaactccc agctccgctc catcacccac agctttgtgg gaaggccttc cgaaggggcc
     7681 cagttagagg actacttccc cgactccgag aaccctgaag tggggggcct catggcagtc
     7741 ctggctgtga ttggaggcat cgatggtcgc ctgcgcctgg gcggtcaagt tatgcacgat
     7801 gagtttggag aaggcactgt gactcgcatc accccaaagg gcaaaatcac cgtgcagttc
     7861 tctgacatgc ggacgtgtcg cgtttgccca ttgaatcagc tgaaaccact ccctgccgtg
     7921 gcctttaatg tgaacaacct gcccttcaca gagcccatgc tgtctgtctg ggctcagttg
     7981 gtgaacctcg ctggaagcaa gttagaaaag cacaaaataa agaaatcgac taaacaggcc
     8041 tttgcaggac aagtggacct ggacctgctg cggtgccagc agttgaagct atacatcctg
     8101 aaagcaggtc gggcgctgct ctcccaccag gataaactgc ggcagatcct gtctcagcca
     8161 gctgttcagg agactggaac tgttcacaca gatgatggag cagtggtatc acctgacctt
     8221 ggggacatgt ctcctgaagg gccgcagccc cccatgatcc tcttgcagca gctgctggcc
     8281 tcggccaccc agccgtctcc tgtgaaggcc atatttgata aacaggaact tgaggctgct
     8341 gcactggccg tttgccagtg cttggctgtg gagtccactc acccttcgag cccaggattt
     8401 gaagactgca gctccagtga ggccaccacg cctgtcgccg tgcagcacat ccgccctgcc
     8461 agagtgaaga ggcgcaagca gtcgcccgtt cccgctctgc cgatcgtggt gcagctcatg
     8521 gagatgggat tttccagaag gaacatcgag tttgccctga agtctctcac tggtgcttcc
     8581 gggaatgcat ccagcttgcc tggtgtggaa gccttggtcg ggtggctgct ggaccactcc
     8641 gacatacagg tcacggagct ctcagatgca gacacggtgt ccgacgagta ttctgacgag
     8701 gaggtggtgg aggacgtgga tgatgccgcc tactccatgt ctactggtgc tgttgtgacg
     8761 gagagccaga cgtacaaaaa acgagctgat ttcttgagta atgatgatta tgctgtatat
     8821 gtgagagaga atattcaggt gggaatgatg gttagatgct gccgagcgta tgaagaagtg
     8881 tgcgaaggtg atgttggcaa agtcatcaag ctggacagag atggattgca tgatctcaat
     8941 gtgcagtgtg actggcagca gaaagggggc acctactggg ttaggtacat tcatgtggaa
     9001 cttataggct atcctccacc aagttcttct tctcacatca agattggtga taaagtgcgg
     9061 gtcaaagcct ctgtcaccac accaaaatac aaatggggat ctgtgactca tcagagtgtg
     9121 ggggttgtga aggctttcag tgccaatgga aaagatatca ttgtcgactt tccccagcag
     9181 tctcactgga ctgggttgct atcagaaatg gagttggtac ccagtattca tcctggggtt
     9241 acgtgtgatg gatgtcagat gtttcctatc aatggatcca gattcaaatg cagaaactgt
     9301 gatgactttg atttttgtga aacgtgtttc aagaccaaaa aacacaatac caggcataca
     9361 tttggcagaa taaatgaacc aggtcagtct gcggtatttt gtggccgttc tggaaaacag
     9421 ctgaagcgtt gccacagcag ccagccaggc atgctgctgg acagctggtc ccgcatggtg
     9481 aagagcctga atgtgtcgtc ctccgtgaac caggcatccc gtctcattga cggcagcgag
     9541 ccctgctggc agtcatcggg gtcgcaagga aagcactgga ttcgtttgga gattttccca
     9601 gatgttcttg ttcatagatt aaaaatgatc gtagatcctg ctgacagtag ctacatgccg
     9661 tccctggttg tagtgtcagg tggaaattcc ctgaataacc ttattgaact aaagacaatc
     9721 aatattaacc cttctgacac cacagtgccc cttctgaatg actgcacaga gtatcacagg
     9781 tatattgaaa ttgctataaa gcagtgcagg agctcaggaa tcgattgtaa aatccatggt
     9841 ctcatcctgc tgggacggat ccgtgcagaa gaggaagatt tggctgcagt tcctttctta
     9901 gcttcggata atgaagagga ggaggatgag aaaggcaaca gcggaagcct cattagaaag
     9961 aaggctgctg ggctggaatc agcagctacg ataagaacca aggtgtttgt gtggggcctg
    10021 aatgacaagg accagctggg cgggctgaaa ggctccaaga taaaggttcc ttcgttctct
    10081 gagacactgt cagctttgaa tgtggtacag gtggctggtg gatctaaaag tttgtttgca
    10141 gtgactgtgg aagggaaggt gtatgcctgt ggagaagcca cgaatggccg gctggggctg
    10201 ggcatttcca gcgggacggt gcccatccca cggcagatca cagctctcag cagctacgtg
    10261 gtcaagaagg tggctgttca ctcaggtggc cggcacgcga cggctttaac tgtcgatgga
    10321 aaagtgtttt cgtggggcga aggtgacgat ggaaaacttg gacacttcag cagaatgaac
    10381 tgtgacaaac caaggctgat cgaggccctg aaaaccaagc gtatccggga tatcgcctgt
    10441 gggagctcgc acagcgcagc cctcacatcc agcggagaac tgtacacctg gggcctcggc
    10501 gagtacggcc ggctgggaca tggggataat acgacacagc taaagcccaa aatggtgaaa
    10561 gtccttctcg gtcacagagt aatccaggtt gcatgtggga gtagagacgc gcagaccctg
    10621 gctctgaccg atgaaggttt ggtattttcc tggggtgatg gtgactttgg aaaactgggc
    10681 cggggcggaa gtgaaggctg taacattccc cagaacattg agagactaaa tggacagggg
    10741 gtgtgccaga ttgagtgtgg agctcagttc tccctggcgc tcaccaagtc tggagtggtg
    10801 tggacatggg gaaaggggga ttacttcaga ttgggccacg gctctgacgt gcacgtgcgg
    10861 aaaccacagg tggtggaagg gctgagaggg aagaagatcg tgcatgtggc tgtcggggcc
    10921 ctgcactgcc tggcggtcac ggactcgggg caggtgtatg cttggggtga caacgaccac
    10981 ggccagcagg gcaatggcac gaccacggtt aacaggaagc ccacactcgt gcaaggctta
    11041 gaaggccaga agatcacacg cgtggcttgt gggtcgtccc acagtgtggc gtggacaact
    11101 gtggatgtgg ccacgccctc tgtccacgag cccgtcctct tccagactgc aagagaccct
    11161 ttaggtgctt cctatttagg cgtgccttca gatgctgatt cttctgctgc cagtaataaa
    11221 ataagtggtg caagtaattc taagccaaat cgcccttctc ttgccaagat tctcttgtca
    11281 ttggatggaa atctggccaa acagcaggcc ttatcacata ttcttacagc attgcaaatc
    11341 atgtatgcca gagatgctgt tgtcggggcc ctgatgccgg ccgccatgat cgccccggtg
    11401 gagtgcccct cgttctcctc ggcggcccct tccgacgcat ctgcgatggc tagtcccatg
    11461 aatggagaag aatgcatgct ggctgttgat atcgaagaca gactgagtcc aaatccatgg
    11521 caagaaaaga gagagattgt ttcctctgag gacgcagtga ccccctctgc agtgactccg
    11581 tcggccccct cagcctccgc tcggcctttt atcccagtga cggatgacct gggagccgca
    11641 agcatcattg cagaaaccat gaccaaaacc aaagaggatg ttgaaagcca aaataaagca
    11701 gcaggtccgg agcctcaggc cttggatgag ttcaccagtc tgctgattgc ggatgacact
    11761 cgtgtggtgg tagacctgct caagctgtca gtgtgcagcc gggccgggga caggggcagg
    11821 gatgtgctct ccgcggtgct ttccggcatg gggaccgcct acccacaggt ggcagatatg
    11881 ctgttggagc tctgtgtcac cgagttggag gatgtggcca cagactcgca gagcggccgc
    11941 ctctcttctc agcctgtggt ggtggagagt agccaccctt acaccgacga cacctccacc
    12001 agtggcacag tgaagatacc aggtgcagaa ggactcaggg tagaatttga ccggcagtgc
    12061 tccacagaga ggcgccacga ccctctcaca gtcatggacg gcgtcaacag gatcgtctcc
    12121 gtgcggtcag gccgagagtg gtccgactgg tccagcgagc tgcgcatccc aggggatgag
    12181 ttaaagtgga agttcatcag cgatgggtct gtgaatggct ggggctggcg cttcaccgtc
    12241 tatcccatca tgccagctgc tggccctaaa gaactcctct ctgaccgctg cgtcctctcc
    12301 tgtccatcca tggacttggt gacgtgtctg ttagacttcc gactcaacct tgcctctaac
    12361 agaagcatcg tccctcgcct tgcggcctcg ctggcagctt gtgcacagct gagtgcccta
    12421 gctgccagtc acagaatgtg ggcccttcag agactgagga agctgcttac aactgaattt
    12481 gggcagtcaa ttaacataaa taggctgctt ggagaaaatg atggggaaac aagagctttg
    12541 agttttacag gtagtgctct tgctgctttg gtgaaaggtc ttccagaagc tttgcaaagg
    12601 cagtttgaat atgaagatcc tattgtgagg ggtggcaaac agctgctcca cagcccattc
    12661 tttaaggtac tggtagctct tgcttgtgac ctggagctgg acactctgcc ttgctgtgcc
    12721 gagacgcaca agtgggcctg gttccggagg tactgcatgg cctcccgtgt tgctgtggcc
    12781 cttgacaaaa gaacaccgtt gccccgtctg tttcttgatg aggtggctaa gaaaattcgt
    12841 gaattaatgg cagacagcga aaacatggat gttctgcatg agagccatga catttttaaa
    12901 agagagcaag acgaacaact tgtgcagtgg atgaacaggc gaccagatga ctggactctc
    12961 tctgctggtg gcagtggaac aatttatgga tggggacata atcacagggg ccagctcggg
    13021 ggcattgaag gcgcaaaagt caaagttccc actccctgtg aagcccttgc aactctcaga
    13081 cccgtgcagt taatcggagg ggaacagacc ctctttgctg tgacggctga tgggaagctg
    13141 tatgccactg ggtatggtgc aggtggcaga ctaggcattg gagggacaga gtcggtgtcc
    13201 accccaacat tgcttgaatc cattcagcat gtgtttatta agaaagtagc tgtgaactct
    13261 ggaggaaagc actgccttgc cctgtcttca gaaggagaag tttactcttg gggtgaggca
    13321 gaagatggga agttggggca tggcaacaga agtccgtgtg accgccctcg tgtcatcgag
    13381 tctctgagag gaattgaagt ggtcgatgtt gctgctggcg gagcccacag cgcctgtgtc
    13441 acagcagccg gggacctcta cacatggggc aaaggccgct acggccggct ggggcacagc
    13501 gacagtgagg accagctgaa gccgaagctg gtggaggcgc tgcagggcca ccgtgtggtt
    13561 gacatcgcct gtggcagtgg agatgcccag accctctgcc tcacagatga cgacactgtc
    13621 tggtcctggg gggacgggga ctacggcaag ctcggccggg gaggcagcga tggctgtaaa
    13681 gtgcctatga agattgattc tcttactggt cttggagtag ttaaagtgga atgcggatcc
    13741 cagttttctg ttgcccttac caaatctgga gctgtttata cctggggcaa aggcgattat
    13801 cacaggttgg gccatggatc agatgaccat gttcgaaggc ctcggcaggt ccaagggttg
    13861 caggggaaga aagtcatcgc catcgccact ggctccctgc actgtgtgtg ctgcacagag
    13921 gatggtgagg tttatacatg gggcgacaat gatgagggac aactgggaga cggaaccacc
    13981 aatgccatcc agaggcctcg gttggtagct gcccttcagg gtaagaaggt caaccgtgtg
    14041 gcctgtggct cagcacatac cctcgcctgg tcgaccagca agcccgccag tgctggcaaa
    14101 ctccctgcac aggtccccat ggagtacaat cacctgcagg agatccccat cattgcgctg
    14161 aggaaccgtc tgctgctgct gcaccacctc tccgagctct tctgcccctg catccccatg
    14221 ttcgacctgg aaggctcgct cgacgaaact ggactcgggc cttctgttgg gttcgacact
    14281 ctccgaggaa ttctgatatc ccagggaaag gaggcggctt tccggaaagt agtacaagca
    14341 actatggtac gcgatcgtca gcatggcccc gtcgtggagc tgaaccgcat ccaggtcaaa
    14401 cgatcaagga gcaaaggcgg gctggccggc cccgacggca ccaagtctgt ctttgggcag
    14461 atgtgtgcta agatgagctc gtttggtccc gacagcctcc tccttcctca ccgtgtctgg
    14521 aaagtcaagt ttgtgggtga atctgtggat gactgtgggg gcggctacag cgagtccata
    14581 gctgagatct gtgaggagct gcagaacgga ctcacgcccc tgctgatcgt gacacccaac
    14641 gggagggatg agtctggggc caaccgagac tgctacctgc tcagcccggc cgccagagca
    14701 cccgtgcaca gcagcatgtt ccgcttcctg ggtgtgttgc tgggcattgc catccgaacc
    14761 gggagtcccc tgagcctcaa ccttgccgag cctgtctgga agcagctggc tgggatgagc
    14821 ctcaccatcg cggacctcag tgaggttgat aaggatttta ttcctggact catgtacatc
    14881 cgagacaatg aagccacctc agaggagttt gaagccatga gcctgccctt cacagtgcca
    14941 agtgccagtg gccaggacat tcagttgagc tccaagcaca cacacatcac cctggacaac
    15001 cgcgcggagt acgtgcggct ggcgataaac tatagactcc atgaatttga tgagcaggtg
    15061 gctgctgttc gggaaggaat ggcccgcgtt gtgcctgttc ccctcctctc tctgttcacc
    15121 ggctacgaac tggagacgat ggtgtgtggc agccctgaca tcccgctgca ccttctcaag
    15181 tcggtggcca cctataaagg catcgagcct tccgcatcgc tgatccagtg gttctgggag
    15241 gtgatggagt ccttctccaa cacagagcgc tctcttttcc ttcgcttcgt ctggggccgg
    15301 acgaggctgc ccaggaccat cgccgacttc cggggccgag acttcgtcat ccaggtgttg
    15361 gataaataca accctccaga ccacttcctc cctgagtcct acacctgttt cttcttgctg
    15421 aagctgccca ggtattcctg caagcaggtg ctggaggaga agctcaagta cgccatccac
    15481 ttctgcaagt ccatagacac agatgactac gctcgcatcg cacttacagg agagccagcc
    15541 gccgacgaca gcagcgacga ttcagataac gaggatgtcg actcctttgc ttcggactct
    15601 acacaagatt atttaacagg acactaagtt taaacccgct gatcagcctc gactgtgcct
    15661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    15721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    15781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    15841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    15901 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    15961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    16021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    16081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    16141 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
    16201 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
    16261 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
    16321 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
    16381 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
    16441 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
    16501 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
    16561 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
    16621 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
    16681 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
    16741 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
    16801 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
    16861 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
    16921 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
    16981 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
    17041 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
    17101 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
    17161 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
    17221 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
    17281 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    17341 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    17401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    17461 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    17521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    17581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    17641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    17701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    17761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    17821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    17881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    17941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    18001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    18061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    18121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    18181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    18241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    18301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    18361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    18421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    18481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    18541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    18601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    18661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    18721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    18781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    18841 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    18901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    18961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    19021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    19081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    19141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    19201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    19261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    19321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    19381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    19441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    19501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    19561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    19621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    19681 gtgccacctg acgtc
//