pESC-URA

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Agilent 安捷伦
载体名称: pESC-URA, pESC URA
Gene accession NO. : AF063585
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1和GAL10
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6631 bp
5' 测序引物及序列: GAL1-F: ATTTTCGGTTTGTATTACTTC;
GAL10-F: GGTGGTAATGCCATGTAATATG
3' 测序引物及序列: GALI-R: GTTCTTAATACTAACATAACT
GAL10-R: GGCAAGGTAGACAAGCCGACAAC
载体标签: C-Flag, C-Myc
载体抗性: 氨苄
筛选标记: URA3
备注: 利用半乳糖诱导,可以同时使两个基因在酿酒酵母中表达。
产品目录号: 217454
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pESC-URA载体图谱

pESC-URA多克隆位点

载体简介

载体序列

LOCUS       pESC-URA                6631 bp    DNA     circular SYN 24-SEP-2005
DEFINITION  Cloning vector pESC-URA, complete sequence.
ACCESSION   AF063585
VERSION     AF063585.2  GI:6446607
SOURCE      Cloning vector pESC-URA.
  ORGANISM  Cloning vector pESC-URA
            artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Cloning vector pESC-URA"
                     /mol_type="genomic DNA"
                     /specific_host="Escherichia coli K12"
                     /db_xref="taxon:76664"
                     /vntifkey="98"
     CDS             complement(4232..5089)
                     /vntifkey="4"
                     /label=AmpR
     rep_origin      5223..6378
                     /vntifkey="33"
                     /label=2\micron\ori
     CDS             417..1217
                     /vntifkey="4"
                     /label=URA3
                     /note="yeast URA3 ORF"
     terminator      complement(1885..2049)
                     /vntifkey="43"
                     /label=ADH1\terminator
     terminator      3038..3227
                     /vntifkey="43"
                     /label=CYC1\terminator
     rep_origin      complement(1483..1789)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        complement(2268..2479)
                     /vntifkey="29"
                     /label=GAL10\promoter
     promoter        2480..2934
                     /vntifkey="29"
                     /label=GAL1\promoter
     misc_feature    2976..3011
                     /vntifkey="21"
                     /label=c-myc\tag
     misc_feature    complement(2198..2224)
                     /vntifkey="21"
                     /label=FLAG\tag
     misc_feature    complement(2180..2263)
                     /vntifkey="21"
                     /label=MCS1
     misc_feature    2936..3033
                     /vntifkey="21"
                     /label=MCS2
     rep_origin      3414..4081
                     /vntifkey="33"
                     /label=pUC\ori
     primer          2770..2788
                     /vntifkey="27"
                     /label=oligo\SapA
     primer          2837..2857
                     /vntifkey="27"
                     /label=Sequence\Primer
BASE COUNT     1900 a      1403 c      1474 g      1854 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc 
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg 
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc 
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt 
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat 
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca 
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg 
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg 
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca 
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac 
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa 
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg 
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct 
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac 
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg 
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa 
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa 
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac 
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac 
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat 
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     1801 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 
     1861 ttcgctatta cgccagctga attggagcga cctcatgcta tacctgagaa agcaacctga 
     1921 cctacaggaa agagttactc aagaataaga attttcgttt taaaacctaa gagtcacttt 
     1981 aaaatttgta tacacttatt ttttttataa cttatttaat aataaaaatc ataaatcata 
     2041 agaaattcgc ttatttagaa gtgtcaacaa cgtatctacc aacgatttga cccttttcca 
     2101 tcttttcgta aatttctggc aaggtagaca agccgacaac cttgattgga gacttgacca 
     2161 aacctctggc gaagaattgt taattaagag ctcagatctt atcgtcgtca tccttgtaat 
     2221 ccatcgatac tagtgcggcc gccctttagt gagggttgaa ttcgaatttt caaaaattct 
     2281 tacttttttt ttggatggac gcaaagaagt ttaataatca tattacatgg cattaccacc 
     2341 atatacatat ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa 
     2401 gagccccatt atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa 
     2461 ctgctcattg ctatattgaa gtacggatta gaagccgccg agcgggtgac agccctccga 
     2521 aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt 
     2581 gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg 
     2641 aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa 
     2701 caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc 
     2761 gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt 
     2821 aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat 
     2881 caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa accccggatc 
     2941 cgtaatacga ctcactatag ggcccgggcg tcgacatgga acagaagttg atttccgaag 
     3001 aagacctcga gtaagcttgg taccgcggct agctaagatc cgctctaacc gaaaaggaag 
     3061 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa 
     3121 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta 
     3181 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc cagctgcatt 
     3241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     3301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     3361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     3421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     3481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     3541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     3601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     3661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     3721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     3781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     3841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     3901 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     3961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     4021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     4081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     4141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     4201 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     4261 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     4321 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     4381 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     4441 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     4501 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     4561 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     4621 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     4681 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     4741 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     4801 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     4861 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     4921 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     4981 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     5041 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     5101 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     5161 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     5221 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt 
     5281 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt 
     5341 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa 
     5401 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc 
     5461 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc 
     5521 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg 
     5581 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg 
     5641 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta 
     5701 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat 
     5761 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag 
     5821 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca 
     5881 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa 
     5941 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg 
     6001 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga 
     6061 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc 
     6121 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc 
     6181 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt 
     6241 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac 
     6301 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg 
     6361 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct 
     6421 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta 
     6481 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct 
     6541 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat 
     6601 aaaaataggc gtatcacgag gccctttcgt c 
//

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