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p416 GPD载体基本信息
p416 GPD载体图谱质粒图谱和多克隆位点信息
p416 GPD载体简介
p416 GPD载体序列
LOCUS P416GPD 5778 bp DNA circular SYN 10-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5778)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 10, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5778
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 70..573
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
promoter 610..714
/gene="bla"
/label=AmpR promoter
CDS 715..1575
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1746..2334
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2658..2688
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2696..2712
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2720..2736
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2757..2775
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 2800..3443
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
primer_bind 3449..3465
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3499..3515)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 3518..3765
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
promoter complement(3784..3802)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3812..3828)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3973..4428
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(4559..5362)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(5363..5578)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
2761 aaccctcact aaagggaaca aaagctggag ctcagtttat cattatcaat actcgccatt
2821 tcaaagaata cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat
2881 tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa
2941 tatataacat cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg
3001 gagcccgctt tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt
3061 ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg
3121 cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa
3181 tgatgacaca aggcaattga cccacgcatg tatctatctc attttcttac accttctatt
3241 accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga
3301 aattattccc ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg
3361 taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt
3421 aaaacaccag aacttagttt cgacggattc tagaactagt ggatcccccg ggctgcagga
3481 attcgatatc aagcttatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc
3541 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
3601 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
3661 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
3721 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt
3781 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
3841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
3901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
3961 gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
4021 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
4081 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
4141 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
4201 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
4261 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
4321 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
4381 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc tgatgcggta
4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt
4501 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt
4561 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac
4621 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag
4681 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt
4741 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca
4801 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag
4861 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca
4921 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac
4981 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac
5041 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga
5101 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa
5161 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa
5221 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct
5281 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt
5341 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca
5401 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata
5461 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa
5521 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt
5581 gaaaagctgt ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
5641 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
5701 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
5761 catcaccgaa acgcgcga
//
