| 质粒名称: | pAAVLINK-EF1a-HA-5'-ABCC6 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001677 |
| 产品用途: | 5' AAVLINK plasmid for ABCC6 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ABCC6 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-ABCC6
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-ABCC6载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ABCC6质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ABCC6载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ABCC6质粒载体简介
pAAVLINK-EF1a-HA-5'-ABCC6质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ABCC6 5855 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5855)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5855
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2995
/codon_start=1
/label=ABCC6
/translation="MAAPAEPCAGQGVWNQTEPEPAATSLLSLCFLRTAGVWVPPMYLW
VLGPIYLLFIHHHGRGYLRMSPLFKAKMVLGFALIVLCTSSVAVALWKIQQGTPEAPEF
LIHPTVWLTTMSFAVFLIHTERKKGVQSSGVLFGYWLLCFVLPATNAAQQASGAGFQSD
PVRHLSTYLCLSLVVAQFVLSCLADQPPFFPEDPQQSNPCPETGAAFPSKATFWWVSGL
VWRGYRRPLRPKDLWSLGRENSSEELVSRLEKEWMRNRSAARRHNKAIAFKRKGGSGMK
APETEPFLRQEGSQWRPLLKAIWQVFHSTFLLGTLSLIISDVFRFTVPKLLSLFLEFIG
DPKPPAWKGYLLAVLMFLSACLQTLFEQQNMYRLKVLQMRLRSAITGLVYRKVLALSSG
SRKASAVGDVVNLVSVDVQRLTESVLYLNGLWLPLVWIVVCFVYLWQLLGPSALTAIAV
FLSLLPLNFFISKKRNHHQEEQMRQKDSRARLTSSILRNSKTIKFHGWEGAFLDRVLGI
RGQELGALRTSGLLFSVSLVSFQVSTFLVALVVFAVHTLVAENAMNAEKAFVTLTVLNI
LNKAQAFLPFSIHSLVQARVSFDRLVTFLCLEEVDPGVVDSSSSGSAAGKDCITIHSAT
FAWSQESPPCLHRINLTVPQGCLLAVVGPVGAGKSSLLSALLGELSKVEGFVSIEGAVA
YVPQEAWVQNTSVVENVCFGQELDPPWLERVLEACALQPDVDSFPEGIHTSIGEQ"
misc_feature 901..906
/label=SDS
misc_feature 1430..1435
/label=SDS
misc_feature 1681..1686
/label=SDS
misc_feature 1798..1803
/label=SDS
misc_feature 2131..2136
/label=SDS
misc_feature 2178..2183
/label=SDS
misc_feature 2335..2340
/label=SDS
misc_feature 2773..2778
/label=SDS
misc_feature 2841..2846
/label=SDS
intron 2996..3036
/label=Splicing Donor
misc_feature 3040..3073
/label=lox2272-JT1571
repeat_region 3118..3258
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3118..3247
/label=AAV2 ITR
rep_origin 3333..3788
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4070..4174
/gene="bla"
/label=AmpR promoter
CDS 4175..5035
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5206..5794
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccgcgcct gctgagccct gcgcggggca
781 gggggtctgg aaccagacag agcctgaacc tgccgccacc agcctgctga gcctgtgctt
841 cctgagaaca gcaggggtct gggtaccccc catgtacctc tgggtccttg gtcccatcta
901 cctcctcttc atccaccacc atggccgggg ctacctccgg atgtccccac tcttcaaagc
961 caagatggtg cttggattcg ccctcatagt cctgtgtacc tccagcgtgg ctgtcgctct
1021 ttggaaaatc caacagggaa cgcctgaggc cccagaattc ctcattcatc ctactgtgtg
1081 gctcaccacg atgagcttcg cagtgttcct gattcacacc gagaggaaaa agggagtcca
1141 gtcatctgga gtgctgtttg gttactggct tctctgcttt gtcttgccag ctaccaacgc
1201 tgcccagcag gcctccggag cgggcttcca gagcgaccct gtccgccacc tgtccaccta
1261 cctatgcctg tctctggtgg tggcacagtt tgtgctgtcc tgcctggcgg atcaaccccc
1321 cttcttccct gaagaccccc agcagtctaa cccctgtcca gagactgggg cagccttccc
1381 ctccaaagcc acgttctggt gggtttctgg cctggtctgg aggggataca ggaggccact
1441 gagaccaaaa gacctctggt cgcttgggag agaaaactcc tcagaagaac ttgtttcccg
1501 gcttgaaaag gagtggatga ggaaccgcag tgcagcccgg aggcacaaca aggcaatagc
1561 atttaaaagg aaaggcggca gtggcatgaa ggctccagag accgagccct tcctacggca
1621 agaagggagc cagtggcgcc cactgctgaa ggccatctgg caggtgttcc attctacctt
1681 cctcctgggg accctcagcc tcatcatcag tgatgtcttc aggttcactg tccccaagct
1741 gctcagcctt ttcctggagt ttattggtga tcccaagcct ccagcctgga agggctacct
1801 cctcgccgtg ctgatgttcc tctcagcctg cctgcaaacg ctgtttgagc agcagaacat
1861 gtacaggctc aaggtgctgc agatgaggtt gcggtcggcc atcactggcc tggtgtacag
1921 aaaggtcctg gctctgtcca gcggctccag aaaggccagt gcggtgggtg atgtggtcaa
1981 tctggtgtcc gtggacgtgc agcggctgac cgagagcgtc ctctacctca acgggctgtg
2041 gctgcctctc gtctggatcg tggtctgctt cgtctatctc tggcagctcc tggggccctc
2101 cgccctcact gccatcgctg tcttcctgag cctcctccct ctgaatttct tcatctccaa
2161 gaaaaggaac caccatcagg aggagcaaat gaggcagaag gactcacggg cacggctcac
2221 cagctctatc ctcaggaact cgaagaccat caagttccat ggctgggagg gagcctttct
2281 ggacagagtc ctgggcatcc gaggccagga gctgggcgcc ttgcggacct ccggcctcct
2341 cttctctgtg tcgctggtgt ccttccaagt gtctacattt ctggtcgcac tggtggtgtt
2401 tgctgtccac actctggtgg ccgagaatgc tatgaatgca gagaaagcct ttgtgactct
2461 cacagttctc aacatcctca acaaggccca ggctttcctg cccttctcca tccactccct
2521 cgtccaggcc cgggtgtcct ttgaccgtct ggtcaccttc ctctgcctgg aagaagttga
2581 ccctggtgtc gtagactcaa gttcctctgg aagcgctgcc gggaaggatt gcatcaccat
2641 acacagtgcc accttcgcct ggtcccagga aagccctccc tgcctccaca gaataaacct
2701 cacggtgccc cagggctgtc tgctggctgt tgtcggtcca gtgggggcag ggaagtcctc
2761 cctgctgtcc gccctccttg gggagctgtc aaaggtggag gggttcgtga gcatcgaggg
2821 tgctgtggcc tacgtgcccc aggaggcctg ggtgcagaac acctctgtgg tagagaatgt
2881 gtgcttcggg caggagctgg acccaccctg gctggagaga gtactagaag cctgtgccct
2941 gcagccagat gtggacagct tccctgaggg aatccacact tcaattgggg agcaggtaag
3001 tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta taggatactt
3061 tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
3121 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
3181 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
3241 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
3301 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
3361 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
3421 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3481 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3541 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3601 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
3661 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
3721 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
3781 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
3841 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
3901 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
3961 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4021 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
4141 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
4201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
4261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
4321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
4381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
4441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
4501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
4561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
4621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
4681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
4741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
4801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
4861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
4921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
4981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
5041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
5101 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
5161 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
5221 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
5281 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
5341 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
5401 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
5461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
5521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
5581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
5641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
5701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
5761 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
5821 gttcctggcc ttttgctggc cttttgctca catgt
//
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pAAVLINK-EF1a-HA-5'-ABCC6质粒载体应用举例
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