| 质粒名称: | mApple-N1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 54567 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mApple-N1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | mApple（主干上的C终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mApple-N1 was a gift from Michael Davidson (Addgene plasmid # 54567 ; http://n2t.net/addgene:54567 ; RRID:Addgene_54567) |
| 参考文献部分: | Improving the photostability of bright monomeric orange and red fluorescent proteins. Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY. Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. 10.1038/nmeth.1209 PubMed 18454154 |
买家导航
mAppleN1载体基本信息
mAppleN1载体图谱质粒图谱和多克隆位点信息
mAppleN1载体简介
mAppleN1载体序列
LOCUS mApple-N1 4723 bp ds-DNA circular SYN 24-JUN-2019
DEFINITION Localization: N1 Cloning Vector, Excitation: 568, Emission: 592.
ACCESSION .
VERSION .
KEYWORDS mApple-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4723)
AUTHORS Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson
MW, Tsien RY
TITLE Improving the photostability of bright monomeric orange and red
fluorescent proteins.
JOURNAL Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub
2008 May 4.
PUBMED 18454154
REFERENCE 2 (bases 1 to 4723)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 24, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4723
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 598..678
/label=MCS
/note="multiple cloning site"
regulatory 680..689
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 686..1396
/codon_start=1
/product="photostable monomeric derivative of DsRed (Shaner
et al., 2008)"
/label=mApple
/note="mammalian codon-optimized"
/translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind 1099..1118
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 1518..1639
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1555..1574)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1609..1628
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1646..2101)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1783..1804)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1995..2014
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2128..2232
/gene="bla"
/label=AmpR promoter
promoter 2234..2591
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2257..2277)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2442..2577
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2504..2523
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2626..3420
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2680..2699)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3290..3309
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3608..3627)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3652..3699
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4028..4616
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4517..4536
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
661 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat
721 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
781 cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa
841 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
901 cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt
961 ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt
1021 taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac
1081 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga
1141 ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct
1201 gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt
1261 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga
1321 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
1381 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
1441 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
1501 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
1621 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
1681 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
1741 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
1801 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
1861 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
1921 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
1981 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
2041 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
2101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
2161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
2221 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
2281 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2341 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2401 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
2461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
2521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
2581 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
2641 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
2701 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
2761 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
2821 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
2881 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
2941 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
3001 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
3061 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
3121 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
3181 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
3241 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
3301 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
3361 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
3421 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
3481 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
3541 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
3601 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
3661 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
3721 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
3781 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
3841 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
3901 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
3961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
4021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
4081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
4141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
4201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
4261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
4321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
4381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
4441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
4501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
4561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
4621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
4681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgc
//
