pFUPI hT5-S322-Cyp

LOCUS       pFUPI hT5-S322-Cyp               11868 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human TRIM5 CypA fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pFUPI hT5-S322-Cyp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, 
            Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J
  TITLE     Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins 
            engineered from human components.
  JOURNAL   J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub
            2009 Sep 8.
  PUBMED    19741300
REFERENCE   2  (bases 1 to 11868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..3821
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3658..3677
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3860..4459
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3860..3879)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4356..4376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4468..5041
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4635..4652)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4862..4881
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6560..7148
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6613..6633)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7031..7042)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7151..7167)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7152..7168)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7673..7853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7879..7896)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7885..8109
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8155..8583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8242..8261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8452..8473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8592..8612)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8597..8926
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8777..8912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8839..8858
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8974..9021
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9040..9414
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9544..9665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9581..9600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9635..9654
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9714..9730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9714..9730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9727..9749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9738..9754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9762..9792)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9807..9828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9945..9962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10116..10704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10196..10215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10875..11735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11498..11517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11736..11840)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
     3841 gactctagct agagccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3901 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3961 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4021 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4081 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4141 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4201 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4261 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4321 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4381 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4441 ccgcaagccc ggtgcctgag aattccgccc ctctccctcc ccccccccta acgttactgg
     4501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4561 gccgtctttt ggcaatgtga gggcccggaa acctggtcct gtcttcttga cgagcattcc
     4621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4681 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4921 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     5041 accggtggtc tagaatggct tctggaatcc tggttaatgt aaaggaggag gtgacctgcc
     5101 ccatctgcct ggaactcctg acacaacccc tgagcctgga ctgcggccac agcttctgcc
     5161 aagcatgcct cactgcaaac cacaagaagt ccatgctaga caaaggagag agtagctgcc
     5221 ctgtgtgccg gatcagttac cagcctgaga acatacggcc taatcggcat gtagccaaca
     5281 tagtggagaa gctcagggag gtcaagttga gcccagaggg gcagaaagtt gatcattgtg
     5341 cacgccatgg agagaaactt ctactcttct gtcaggagga cgggaaggtc atttgctggc
     5401 tttgtgagcg gtctcaggag caccgtggtc accacacgtt cctcacagag gaggttgccc
     5461 gggagtacca agtgaagctc caggcagctc tggagatgct gaggcagaag cagcaggaag
     5521 ctgaagagtt agaagctgac atcagagaag agaaagcttc ctggaagact caaatacagt
     5581 atgacaaaac caacgtcttg gcagattttg agcaactgag agacatcctg gactgggagg
     5641 agagcaatga gctgcaaaac ctggagaagg aggaggaaga cattctgaaa agccttacga
     5701 actctgaaac tgagatggtg cagcagaccc agtccctgag agagctcatc tcagatctgg
     5761 agcatcggct gcaggggtca gtgatggagc tgcttcaggg tgtggatggc gtcataaaaa
     5821 ggacggagaa cgtgaccttg aagaagccag aaacttttcc aaaaaatcaa aggagagtgt
     5881 ttcgagctcc tgatctgaaa ggaatgctag aagtgtttag agagctgaca gatgtccgac
     5941 gctactgggt tgatgtgaca gtggctccaa acaacatttc atgtgctgtc atttctgaag
     6001 ataagagaca agtgagctct gtcaacccca ccgtgttctt cgacattgcc gtcgacggcg
     6061 agcccttggg ccgcgtctcc tttgagctgt ttgcagacaa ggtcccaaag acagcagaaa
     6121 attttcgtgc tctgagcact ggagagaaag gatttggtta taagggttcc tgctttcaca
     6181 gaattattcc agggtttatg tgtcagggtg gtgacttcac acgccataat ggcactggtg
     6241 gcaagtccat ctatggggag aaatttgaag atgagaactt catcctaaag catacgggtc
     6301 ctggcatctt atcgatggca aatgctggac ccaacacaaa tggttcccag tttttcatct
     6361 gcactgccaa gactgagtgg ttggatggca agcatgtggt gtttggcaaa gtgaaagaag
     6421 gcatgaatat tgtggaggcg atggagcgct ttgggtccag gaatggcaag accagcaaga
     6481 agatcaccat tgctgactgt ggacaactcg aataaggatc cgttaacggc gcgccgaatt
     6541 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6601 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6661 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6721 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6781 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6841 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6901 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6961 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7021 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7081 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7141 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     7201 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7261 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7321 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7381 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7441 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7501 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7561 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7621 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     7861 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7921 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7981 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     8041 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     8101 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     8161 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8221 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8281 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8341 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8401 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8461 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8521 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8581 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8701 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8761 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8821 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8881 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8941 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     9001 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     9061 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     9121 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     9181 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     9241 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     9301 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     9361 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     9421 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     9481 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     9541 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9601 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9661 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11161 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11461 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//