pdCas9-humanized

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pdCas9-humanized 
质粒类型: CRISPR-Cas9载体;基因敲除载体;逆病毒载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 10477bp
5' 测序引物及序列: CCGGAATTAGATCTCGCCACCATGGAC
3' 测序引物及序列: CCGGTAGAATTCTATACCTTTCTCTTCT
载体标签: 3x NLS(C ter)
载体抗性: 氨苄青霉素
筛选标记: Puromycin
克隆菌株: DH5alpha
宿主细胞(系): 常规哺乳动物细胞等
备注: Mutation D10A H840A (catalytically inactive);The dCas9 contains an N612K mutation that does not effect function.
产品目录号: #44246
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆病毒

载体质粒图谱和多克隆位点信息

pdCas9-humanized载体图谱



载体简介

载体序列

LOCUS       pdCas9-humanized	10477 bp 	DNA	SYN
DEFINITION  pdCas9-humanized
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="pdCas9-humanized"
                     /mol_type="other DNA"
     misc_feature    654..1403
                     /label="psi_plus_pack"
     misc_feature    990..1397
                     /label="gag"
     misc_feature    1333..1355
                     /label="MSCV_primer"
     misc_feature    1333..1355
                     /label="pLXSN_5_primer"
     misc_feature    1371..1387
                     /label="pBABE_5_primer"
     CDS             1423..5610
                     /label="ORF frame 1"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDEGADPKKKRK
                     VDPKKKRKVDPKKKRKV*"
     CDS             complement(3043..3516)
                     /label="ORF frame 2"
                     /translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
                     SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
                     IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
     misc_feature    5539..5559
                     /label="SV40 NLS"
                     /translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
                     SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
                     IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
     misc_feature    5545..5559
                     /label="NLS"
                     /translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
                     SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
                     IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
     misc_feature    complement(5655..5678)
                     /label="MSCV_rev_primer"
                     /translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
                     SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
                     IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
     CDS             5900..6739
                     /label="ORF frame 2"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     misc_feature    6052..6071
                     /label="mPGK_F_primer"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     CDS             complement(6065..6790)
                     /label="ORF frame 1"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     gene            6140..6739
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     promoter        complement(7518..7536)
                     /label="M13_reverse_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     misc_feature    complement(7535..7557)
                     /label="M13_pUC_rev_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     promoter        complement(7571..7600)
                     /label="lac_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     gene            complement(8683..9543)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLGLQVERPGDEEEENSAADVRF*"
     CDS             complement(8683..9543)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(9585..9613)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(9772..9794)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(9993..10124)
                     /label="lacZ_a"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    10107..10129
                     /label="M13_pUC_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    10257..10276
                     /label="pBRrevBam_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
   61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
  121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
  181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
  241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC
  301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC
  361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA
  421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA
  481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA
  541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG
  601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT
  661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA
  721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA
  781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG
  841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA
  901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG
  961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT
 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA
 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG
 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC
 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC
 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG
 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG
 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCGCCA CCATGGACAA GAAGTATTCT
 1441 ATCGGACTGG CCATCGGGAC TAATAGCGTC GGGTGGGCCG TGATCACTGA CGAGTACAAG
 1501 GTGCCCTCTA AGAAGTTCAA GGTGCTCGGG AACACCGACC GGCATTCCAT CAAGAAAAAT
 1561 CTGATCGGAG CTCTCCTCTT TGATTCAGGG GAGACCGCTG AAGCAACCCG CCTCAAGCGG
 1621 ACTGCTAGAC GGCGGTACAC CAGGAGGAAG AACCGGATTT GTTACCTTCA AGAGATATTC
 1681 TCCAACGAAA TGGCAAAGGT CGACGACAGC TTCTTCCATA GGCTGGAAGA ATCATTCCTC
 1741 GTGGAAGAGG ATAAGAAGCA TGAACGGCAT CCCATCTTCG GTAATATCGT CGACGAGGTG
 1801 GCCTATCACG AGAAATACCC AACCATCTAC CATCTTCGCA AAAAGCTGGT GGACTCAACC
 1861 GACAAGGCAG ACCTCCGGCT TATCTACCTG GCCCTGGCCC ACATGATCAA GTTCAGAGGC
 1921 CACTTCCTGA TCGAGGGCGA CCTCAATCCT GACAATAGCG ATGTGGATAA ACTGTTCATC
 1981 CAGCTGGTGC AGACTTACAA CCAGCTCTTT GAAGAGAACC CCATCAATGC AAGCGGAGTC
 2041 GATGCCAAGG CCATTCTGTC AGCCCGGCTG TCAAAGAGCC GCAGACTTGA GAATCTTATC
 2101 GCTCAGCTGC CGGGTGAAAA GAAAAATGGA CTGTTCGGGA ACCTGATTGC TCTTTCACTT
 2161 GGGCTGACTC CCAATTTCAA GTCTAATTTC GACCTGGCAG AGGATGCCAA GCTGCAACTG
 2221 TCCAAGGACA CCTATGATGA CGATCTCGAC AACCTCCTGG CCCAGATCGG TGACCAATAC
 2281 GCCGACCTTT TCCTTGCTGC TAAGAATCTT TCTGACGCCA TCCTGCTGTC TGACATTCTC
 2341 CGCGTGAACA CTGAAATCAC CAAGGCCCCT CTTTCAGCTT CAATGATTAA GCGGTATGAT
 2401 GAGCACCACC AGGACCTGAC CCTGCTTAAG GCACTCGTCC GGCAGCAGCT TCCGGAGAAG
 2461 TACAAGGAAA TCTTCTTTGA CCAGTCAAAG AATGGATACG CCGGCTACAT CGACGGAGGT
 2521 GCCTCCCAAG AGGAATTTTA TAAGTTTATC AAACCTATCC TTGAGAAGAT GGACGGCACC
 2581 GAAGAGCTCC TCGTGAAACT GAATCGGGAG GATCTGCTGC GGAAGCAGCG CACTTTCGAC
 2641 AATGGGAGCA TTCCCCACCA GATCCATCTT GGGGAGCTTC ACGCCATCCT TCGGCGCCAA
 2701 GAGGACTTCT ACCCCTTTCT TAAGGACAAC AGGGAGAAGA TTGAGAAAAT TCTCACTTTC
 2761 CGCATCCCCT ACTACGTGGG ACCCCTCGCC AGAGGAAATA GCCGGTTTGC TTGGATGACC
 2821 AGAAAGTCAG AAGAAACTAT CACTCCCTGG AACTTCGAAG AGGTGGTGGA CAAGGGAGCC
 2881 AGCGCTCAGT CATTCATCGA ACGGATGACT AACTTCGATA AGAACCTCCC CAATGAGAAG
 2941 GTCCTGCCGA AACATTCCCT GCTCTACGAG TACTTTACCG TGTACAACGA GCTGACCAAG
 3001 GTGAAATATG TCACCGAAGG GATGAGGAAG CCCGCATTCC TGTCAGGCGA ACAAAAGAAG
 3061 GCAATTGTGG ACCTTCTGTT CAAGACCAAT AGAAAGGTGA CCGTGAAGCA GCTGAAGGAG
 3121 GACTATTTCA AGAAAATTGA ATGCTTCGAC TCTGTGGAGA TTAGCGGGGT CGAAGATCGG
 3181 TTCAACGCAA GCCTGGGTAC CTACCATGAT CTGCTTAAGA TCATCAAGGA CAAGGATTTT
 3241 CTGGACAATG AGGAGAACGA GGACATCCTT GAGGACATTG TCCTGACTCT CACTCTGTTC
 3301 GAGGACCGGG AAATGATCGA GGAGAGGCTT AAGACCTACG CCCATCTGTT CGACGATAAA
 3361 GTGATGAAGC AACTTAAACG GAGAAGATAT ACCGGATGGG GACGCCTTAG CCGCAAACTC
 3421 ATCAACGGAA TCCGGGACAA ACAGAGCGGA AAGACCATTC TTGATTTCCT TAAGAGCGAC
 3481 GGATTCGCTA ATCGCAACTT CATGCAACTT ATCCATGATG ATTCCCTGAC CTTTAAGGAG
 3541 GACATCCAGA AGGCCCAAGT GTCTGGACAA GGTGACTCAC TGCACGAGCA TATCGCAAAT
 3601 CTGGCTGGTT CACCCGCTAT TAAGAAGGGT ATTCTCCAGA CCGTGAAAGT CGTGGACGAG
 3661 CTGGTCAAGG TGATGGGTCG CCATAAACCA GAGAACATTG TCATCGAGAT GGCCAGGGAA
 3721 AACCAGACTA CCCAGAAGGG ACAGAAGAAC AGCAGGGAGC GGATGAAAAG AATTGAGGAA
 3781 GGGATTAAGG AGCTCGGGTC ACAGATCCTT AAAGAGCACC CGGTGGAAAA CACCCAGCTT
 3841 CAGAATGAGA AGCTCTATCT GTACTACCTT CAAAATGGAC GCGATATGTA TGTGGACCAA
 3901 GAGCTTGATA TCAACAGGCT CTCAGACTAC GACGTGGACG CCATCGTCCC TCAGAGCTTC
 3961 CTCAAAGACG ACTCAATTGA CAATAAGGTG CTGACTCGCT CAGACAAGAA CCGGGGAAAG
 4021 TCAGATAACG TGCCCTCAGA GGAAGTCGTG AAAAAGATGA AGAACTATTG GCGCCAGCTT
 4081 CTGAACGCAA AGCTGATCAC TCAGCGGAAG TTCGACAATC TCACTAAGGC TGAGAGGGGC
 4141 GGACTGAGCG AACTGGACAA AGCAGGATTC ATTAAACGGC AACTTGTGGA GACTCGGCAG
 4201 ATTACTAAAC ATGTCGCCCA AATCCTTGAC TCACGCATGA ATACCAAGTA CGACGAAAAC
 4261 GACAAACTTA TCCGCGAGGT GAAGGTGATT ACCCTGAAGT CCAAGCTGGT CAGCGATTTC
 4321 AGAAAGGACT TTCAATTCTA CAAAGTGCGG GAGATCAATA ACTATCATCA TGCTCATGAC
 4381 GCATATCTGA ATGCCGTGGT GGGAACCGCC CTGATCAAGA AGTACCCAAA GCTGGAAAGC
 4441 GAGTTCGTGT ACGGAGACTA CAAGGTCTAC GACGTGCGCA AGATGATTGC CAAATCTGAG
 4501 CAGGAGATCG GAAAGGCCAC CGCAAAGTAC TTCTTCTACA GCAACATCAT GAATTTCTTC
 4561 AAGACCGAAA TCACCCTTGC AAACGGTGAG ATCCGGAAGA GGCCGCTCAT CGAGACTAAT
 4621 GGGGAGACTG GCGAAATCGT GTGGGACAAG GGCAGAGATT TCGCTACCGT GCGCAAAGTG
 4681 CTTTCTATGC CTCAAGTGAA CATCGTGAAG AAAACCGAGG TGCAAACCGG AGGCTTTTCT
 4741 AAGGAATCAA TCCTCCCCAA GCGCAACTCC GACAAGCTCA TTGCAAGGAA GAAGGATTGG
 4801 GACCCTAAGA AGTACGGCGG ATTCGATTCA CCAACTGTGG CTTATTCTGT CCTGGTCGTG
 4861 GCTAAGGTGG AAAAAGGAAA GTCTAAGAAG CTCAAGAGCG TGAAGGAACT GCTGGGTATC
 4921 ACCATTATGG AGCGCAGCTC CTTCGAGAAG AACCCAATTG ACTTTCTCGA AGCCAAAGGT
 4981 TACAAGGAAG TCAAGAAGGA CCTTATCATC AAGCTCCCAA AGTATAGCCT GTTCGAACTG
 5041 GAGAATGGGC GGAAGCGGAT GCTCGCCTCC GCTGGCGAAC TTCAGAAGGG TAATGAGCTG
 5101 GCTCTCCCCT CCAAGTACGT GAATTTCCTC TACCTTGCAA GCCATTACGA GAAGCTGAAG
 5161 GGGAGCCCCG AGGACAACGA GCAAAAGCAA CTGTTTGTGG AGCAGCATAA GCATTATCTG
 5221 GACGAGATCA TTGAGCAGAT TTCCGAGTTT TCTAAACGCG TCATTCTCGC TGATGCCAAC
 5281 CTCGATAAAG TCCTTAGCGC ATACAATAAG CACAGAGACA AACCAATTCG GGAGCAGGCT
 5341 GAGAATATCA TCCACCTGTT CACCCTCACC AATCTTGGTG CCCCTGCCGC ATTCAAGTAC
 5401 TTCGACACCA CCATCGACCG GAAACGCTAT ACCTCCACCA AAGAAGTGCT GGACGCCACC
 5461 CTCATCCACC AGAGCATCAC CGGACTTTAC GAAACTCGGA TTGACCTCTC ACAGCTCGGA
 5521 GGGGATGAGG GAGCTGATCC AAAAAAGAAG AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG
 5581 GTAGATCCAA AAAAGAAGAG AAAGGTATAG AATTCTACCG GGTAGGGGAG GCGCTTTTCC
 5641 CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG
 5701 GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGGCT CCGTTCTTTG
 5761 GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC
 5821 AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA
 5881 TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC
 5941 TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA
 6001 GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA
 6061 CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA
 6121 CCTGCAGCCC AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA
 6181 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG
 6241 CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT
 6301 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC
 6361 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG
 6421 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC
 6481 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA
 6541 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC
 6601 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT
 6661 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC
 6721 CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC
 6781 ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT
 6841 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG
 6901 GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG
 6961 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA
 7021 CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC
 7081 CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG
 7141 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC
 7201 TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG GGTACCCGTG TATCCAATAA
 7261 ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA
 7321 GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATGGGTAAC AGTTTCTTGA AGTTGGAGAA
 7381 CAACATTCTG AGGGTAGGAG TCGAATATTA AGTAATCCTG ACTCAATTAG CCACTGTTTT
 7441 GAATCCACAT ACTCCAATAC TCCTGAAATA GTTCATTATG GACAGCGCAG AAGAGCTGGG
 7501 GAGAATTAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
 7561 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
 7621 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
 7681 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
 7741 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
 7801 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
 7861 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
 7921 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
 7981 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
 8041 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
 8101 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
 8161 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
 8221 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
 8281 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
 8341 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
 8401 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
 8461 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
 8521 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
 8581 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
 8641 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
 8701 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
 8761 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
 8821 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
 8881 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
 8941 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
 9001 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
 9061 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
 9121 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
 9181 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
 9241 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
 9301 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
 9361 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
 9421 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
 9481 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
 9541 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
 9601 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
 9661 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
 9721 GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA
 9781 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC
 9841 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC
 9901 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG
 9961 GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG
10021 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG
10081 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCGCAAGG
10141 AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA
10201 CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG
10261 ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG
10321 CGTCCGGCGT AGAGGCGATT AGTCCAATTT GTTAAAGACA GGATATCAGT GGTCCAGGCT
10381 CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGATAAAA
10441 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAA
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng