| 出品公司: | Addgene |
|---|---|
| 载体名称: | pdCas9-humanized |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 10477bp |
| 5' 测序引物及序列: | CCGGAATTAGATCTCGCCACCATGGAC |
| 3' 测序引物及序列: | CCGGTAGAATTCTATACCTTTCTCTTCT |
| 载体标签: | 3x NLS(C ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Puromycin |
| 克隆菌株: | DH5alpha |
| 宿主细胞（系）: | 常规哺乳动物细胞等 |
| 备注: | Mutation D10A H840A (catalytically inactive)；The dCas9 contains an N612K mutation that does not effect function. |
| 产品目录号: | #44246 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pdCas9-humanized 10477 bp DNA SYN
DEFINITION pdCas9-humanized
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10477
/organism="pdCas9-humanized"
/mol_type="other DNA"
misc_feature 654..1403
/label="psi_plus_pack"
misc_feature 990..1397
/label="gag"
misc_feature 1333..1355
/label="MSCV_primer"
misc_feature 1333..1355
/label="pLXSN_5_primer"
misc_feature 1371..1387
/label="pBABE_5_primer"
CDS 1423..5610
/label="ORF frame 1"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKV
LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDEGADPKKKRK
VDPKKKRKVDPKKKRKV*"
CDS complement(3043..3516)
/label="ORF frame 2"
/translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
misc_feature 5539..5559
/label="SV40 NLS"
/translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
misc_feature 5545..5559
/label="NLS"
/translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
misc_feature complement(5655..5678)
/label="MSCV_rev_primer"
/translation="MDKLHEVAISESVALKEIKNGLSALFVPDSVDEFAAKASPSGIS
SPFKLLHHFIVEQMGVGLKPLLDHFPVLEQSESQDNVLKDVLVLLIVQKILVLDDLKQ
IMVGTQACVEPIFDPANLHRVEAFNFLEIVLLQLLHGHLSIGLEQKVHNCLLLFA*"
CDS 5900..6739
/label="ORF frame 2"
/translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
PGA*"
misc_feature 6052..6071
/label="mPGK_F_primer"
/translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT
LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
PGA*"
CDS complement(6065..6790)
/label="ORF frame 1"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
gene 6140..6739
/label="puro (variant)"
/gene="puro (variant)"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
promoter complement(7518..7536)
/label="M13_reverse_primer"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
misc_feature complement(7535..7557)
/label="M13_pUC_rev_primer"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
promoter complement(7571..7600)
/label="lac_promoter"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
gene complement(8683..9543)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
KLGLQVERPGDEEEENSAADVRF*"
CDS complement(8683..9543)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(9585..9613)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(9772..9794)
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(9993..10124)
/label="lacZ_a"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 10107..10129
/label="M13_pUC_fwd_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 10257..10276
/label="pBRrevBam_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT
61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA
121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA
181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT
241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC
301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC
361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA
421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA
481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA
541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG
601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT
661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA
721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA
781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG
841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA
901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG
961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT
1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA
1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG
1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC
1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC
1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG
1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG
1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCGCCA CCATGGACAA GAAGTATTCT
1441 ATCGGACTGG CCATCGGGAC TAATAGCGTC GGGTGGGCCG TGATCACTGA CGAGTACAAG
1501 GTGCCCTCTA AGAAGTTCAA GGTGCTCGGG AACACCGACC GGCATTCCAT CAAGAAAAAT
1561 CTGATCGGAG CTCTCCTCTT TGATTCAGGG GAGACCGCTG AAGCAACCCG CCTCAAGCGG
1621 ACTGCTAGAC GGCGGTACAC CAGGAGGAAG AACCGGATTT GTTACCTTCA AGAGATATTC
1681 TCCAACGAAA TGGCAAAGGT CGACGACAGC TTCTTCCATA GGCTGGAAGA ATCATTCCTC
1741 GTGGAAGAGG ATAAGAAGCA TGAACGGCAT CCCATCTTCG GTAATATCGT CGACGAGGTG
1801 GCCTATCACG AGAAATACCC AACCATCTAC CATCTTCGCA AAAAGCTGGT GGACTCAACC
1861 GACAAGGCAG ACCTCCGGCT TATCTACCTG GCCCTGGCCC ACATGATCAA GTTCAGAGGC
1921 CACTTCCTGA TCGAGGGCGA CCTCAATCCT GACAATAGCG ATGTGGATAA ACTGTTCATC
1981 CAGCTGGTGC AGACTTACAA CCAGCTCTTT GAAGAGAACC CCATCAATGC AAGCGGAGTC
2041 GATGCCAAGG CCATTCTGTC AGCCCGGCTG TCAAAGAGCC GCAGACTTGA GAATCTTATC
2101 GCTCAGCTGC CGGGTGAAAA GAAAAATGGA CTGTTCGGGA ACCTGATTGC TCTTTCACTT
2161 GGGCTGACTC CCAATTTCAA GTCTAATTTC GACCTGGCAG AGGATGCCAA GCTGCAACTG
2221 TCCAAGGACA CCTATGATGA CGATCTCGAC AACCTCCTGG CCCAGATCGG TGACCAATAC
2281 GCCGACCTTT TCCTTGCTGC TAAGAATCTT TCTGACGCCA TCCTGCTGTC TGACATTCTC
2341 CGCGTGAACA CTGAAATCAC CAAGGCCCCT CTTTCAGCTT CAATGATTAA GCGGTATGAT
2401 GAGCACCACC AGGACCTGAC CCTGCTTAAG GCACTCGTCC GGCAGCAGCT TCCGGAGAAG
2461 TACAAGGAAA TCTTCTTTGA CCAGTCAAAG AATGGATACG CCGGCTACAT CGACGGAGGT
2521 GCCTCCCAAG AGGAATTTTA TAAGTTTATC AAACCTATCC TTGAGAAGAT GGACGGCACC
2581 GAAGAGCTCC TCGTGAAACT GAATCGGGAG GATCTGCTGC GGAAGCAGCG CACTTTCGAC
2641 AATGGGAGCA TTCCCCACCA GATCCATCTT GGGGAGCTTC ACGCCATCCT TCGGCGCCAA
2701 GAGGACTTCT ACCCCTTTCT TAAGGACAAC AGGGAGAAGA TTGAGAAAAT TCTCACTTTC
2761 CGCATCCCCT ACTACGTGGG ACCCCTCGCC AGAGGAAATA GCCGGTTTGC TTGGATGACC
2821 AGAAAGTCAG AAGAAACTAT CACTCCCTGG AACTTCGAAG AGGTGGTGGA CAAGGGAGCC
2881 AGCGCTCAGT CATTCATCGA ACGGATGACT AACTTCGATA AGAACCTCCC CAATGAGAAG
2941 GTCCTGCCGA AACATTCCCT GCTCTACGAG TACTTTACCG TGTACAACGA GCTGACCAAG
3001 GTGAAATATG TCACCGAAGG GATGAGGAAG CCCGCATTCC TGTCAGGCGA ACAAAAGAAG
3061 GCAATTGTGG ACCTTCTGTT CAAGACCAAT AGAAAGGTGA CCGTGAAGCA GCTGAAGGAG
3121 GACTATTTCA AGAAAATTGA ATGCTTCGAC TCTGTGGAGA TTAGCGGGGT CGAAGATCGG
3181 TTCAACGCAA GCCTGGGTAC CTACCATGAT CTGCTTAAGA TCATCAAGGA CAAGGATTTT
3241 CTGGACAATG AGGAGAACGA GGACATCCTT GAGGACATTG TCCTGACTCT CACTCTGTTC
3301 GAGGACCGGG AAATGATCGA GGAGAGGCTT AAGACCTACG CCCATCTGTT CGACGATAAA
3361 GTGATGAAGC AACTTAAACG GAGAAGATAT ACCGGATGGG GACGCCTTAG CCGCAAACTC
3421 ATCAACGGAA TCCGGGACAA ACAGAGCGGA AAGACCATTC TTGATTTCCT TAAGAGCGAC
3481 GGATTCGCTA ATCGCAACTT CATGCAACTT ATCCATGATG ATTCCCTGAC CTTTAAGGAG
3541 GACATCCAGA AGGCCCAAGT GTCTGGACAA GGTGACTCAC TGCACGAGCA TATCGCAAAT
3601 CTGGCTGGTT CACCCGCTAT TAAGAAGGGT ATTCTCCAGA CCGTGAAAGT CGTGGACGAG
3661 CTGGTCAAGG TGATGGGTCG CCATAAACCA GAGAACATTG TCATCGAGAT GGCCAGGGAA
3721 AACCAGACTA CCCAGAAGGG ACAGAAGAAC AGCAGGGAGC GGATGAAAAG AATTGAGGAA
3781 GGGATTAAGG AGCTCGGGTC ACAGATCCTT AAAGAGCACC CGGTGGAAAA CACCCAGCTT
3841 CAGAATGAGA AGCTCTATCT GTACTACCTT CAAAATGGAC GCGATATGTA TGTGGACCAA
3901 GAGCTTGATA TCAACAGGCT CTCAGACTAC GACGTGGACG CCATCGTCCC TCAGAGCTTC
3961 CTCAAAGACG ACTCAATTGA CAATAAGGTG CTGACTCGCT CAGACAAGAA CCGGGGAAAG
4021 TCAGATAACG TGCCCTCAGA GGAAGTCGTG AAAAAGATGA AGAACTATTG GCGCCAGCTT
4081 CTGAACGCAA AGCTGATCAC TCAGCGGAAG TTCGACAATC TCACTAAGGC TGAGAGGGGC
4141 GGACTGAGCG AACTGGACAA AGCAGGATTC ATTAAACGGC AACTTGTGGA GACTCGGCAG
4201 ATTACTAAAC ATGTCGCCCA AATCCTTGAC TCACGCATGA ATACCAAGTA CGACGAAAAC
4261 GACAAACTTA TCCGCGAGGT GAAGGTGATT ACCCTGAAGT CCAAGCTGGT CAGCGATTTC
4321 AGAAAGGACT TTCAATTCTA CAAAGTGCGG GAGATCAATA ACTATCATCA TGCTCATGAC
4381 GCATATCTGA ATGCCGTGGT GGGAACCGCC CTGATCAAGA AGTACCCAAA GCTGGAAAGC
4441 GAGTTCGTGT ACGGAGACTA CAAGGTCTAC GACGTGCGCA AGATGATTGC CAAATCTGAG
4501 CAGGAGATCG GAAAGGCCAC CGCAAAGTAC TTCTTCTACA GCAACATCAT GAATTTCTTC
4561 AAGACCGAAA TCACCCTTGC AAACGGTGAG ATCCGGAAGA GGCCGCTCAT CGAGACTAAT
4621 GGGGAGACTG GCGAAATCGT GTGGGACAAG GGCAGAGATT TCGCTACCGT GCGCAAAGTG
4681 CTTTCTATGC CTCAAGTGAA CATCGTGAAG AAAACCGAGG TGCAAACCGG AGGCTTTTCT
4741 AAGGAATCAA TCCTCCCCAA GCGCAACTCC GACAAGCTCA TTGCAAGGAA GAAGGATTGG
4801 GACCCTAAGA AGTACGGCGG ATTCGATTCA CCAACTGTGG CTTATTCTGT CCTGGTCGTG
4861 GCTAAGGTGG AAAAAGGAAA GTCTAAGAAG CTCAAGAGCG TGAAGGAACT GCTGGGTATC
4921 ACCATTATGG AGCGCAGCTC CTTCGAGAAG AACCCAATTG ACTTTCTCGA AGCCAAAGGT
4981 TACAAGGAAG TCAAGAAGGA CCTTATCATC AAGCTCCCAA AGTATAGCCT GTTCGAACTG
5041 GAGAATGGGC GGAAGCGGAT GCTCGCCTCC GCTGGCGAAC TTCAGAAGGG TAATGAGCTG
5101 GCTCTCCCCT CCAAGTACGT GAATTTCCTC TACCTTGCAA GCCATTACGA GAAGCTGAAG
5161 GGGAGCCCCG AGGACAACGA GCAAAAGCAA CTGTTTGTGG AGCAGCATAA GCATTATCTG
5221 GACGAGATCA TTGAGCAGAT TTCCGAGTTT TCTAAACGCG TCATTCTCGC TGATGCCAAC
5281 CTCGATAAAG TCCTTAGCGC ATACAATAAG CACAGAGACA AACCAATTCG GGAGCAGGCT
5341 GAGAATATCA TCCACCTGTT CACCCTCACC AATCTTGGTG CCCCTGCCGC ATTCAAGTAC
5401 TTCGACACCA CCATCGACCG GAAACGCTAT ACCTCCACCA AAGAAGTGCT GGACGCCACC
5461 CTCATCCACC AGAGCATCAC CGGACTTTAC GAAACTCGGA TTGACCTCTC ACAGCTCGGA
5521 GGGGATGAGG GAGCTGATCC AAAAAAGAAG AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG
5581 GTAGATCCAA AAAAGAAGAG AAAGGTATAG AATTCTACCG GGTAGGGGAG GCGCTTTTCC
5641 CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG
5701 GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGGCT CCGTTCTTTG
5761 GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC
5821 AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA
5881 TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC
5941 TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA
6001 GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA
6061 CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA
6121 CCTGCAGCCC AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA
6181 CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG
6241 CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT
6301 CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC
6361 GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG
6421 CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC
6481 GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA
6541 CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC
6601 CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT
6661 CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC
6721 CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC
6781 ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT
6841 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG
6901 GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG
6961 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA
7021 CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC
7081 CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG
7141 CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC
7201 TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG GGTACCCGTG TATCCAATAA
7261 ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA
7321 GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATGGGTAAC AGTTTCTTGA AGTTGGAGAA
7381 CAACATTCTG AGGGTAGGAG TCGAATATTA AGTAATCCTG ACTCAATTAG CCACTGTTTT
7441 GAATCCACAT ACTCCAATAC TCCTGAAATA GTTCATTATG GACAGCGCAG AAGAGCTGGG
7501 GAGAATTAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
7561 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
7621 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
7681 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
7741 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
7801 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
7861 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
7921 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
7981 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
8041 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
8101 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
8161 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
8221 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
8281 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
8341 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
8401 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
8461 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
8521 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
8581 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
8641 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
8701 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
8761 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
8821 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
8881 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
8941 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
9001 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
9061 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
9121 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
9181 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
9241 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
9301 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
9361 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
9421 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
9481 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
9541 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
9601 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
9661 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
9721 GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA
9781 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC
9841 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC
9901 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG
9961 GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG
10021 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG
10081 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCGCAAGG
10141 AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA
10201 CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG
10261 ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG
10321 CGTCCGGCGT AGAGGCGATT AGTCCAATTT GTTAAAGACA GGATATCAGT GGTCCAGGCT
10381 CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGATAAAA
10441 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAA
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