PX386

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX386 或 PX386 Lactobacillus farciminis Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10574 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68323
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX386载体图谱



载体简介

载体序列

LOCUS       PX386 Lactobacillus farciminis Cas9	10574 bp 	DNA	SYN
DEFINITION  PX386 Lactobacillus farciminis Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="PX386 Lactobacillus farciminis Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5265
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI
                     DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD
                     KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT
                     DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE
                     KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT
                     LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI
                     IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG
                     VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE
                     IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA
                     KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK
                     AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN
                     PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV
                     MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD
                     TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG
                     EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT
                     IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK
                     LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD
                     KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY
                     GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA
                     ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL
                     D*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI
                     DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD
                     KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT
                     DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE
                     KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT
                     LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI
                     IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG
                     VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE
                     IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA
                     KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK
                     AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN
                     PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV
                     MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD
                     TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG
                     EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT
                     IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK
                     LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD
                     KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY
                     GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA
                     ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL
                     D*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI
                     DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD
                     KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT
                     DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE
                     KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT
                     LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI
                     IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG
                     VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE
                     IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA
                     KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK
                     AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN
                     PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV
                     MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD
                     TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG
                     EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT
                     IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK
                     LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD
                     KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY
                     GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA
                     ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL
                     D*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMADRISLGLDIGVASVGFSVLDI
                     DKGKVIELGARLFSATVAAGNQDRRDMRGARRLLNRNKQRRQDTGKLFKKFGLIDDFD
                     KGSFYDNFNQNLNPYELRVKGLTEQLTKEELAESLYQIVKHRGISYALKDADVDEGGT
                     DYSVSLKINSQELAEKTPAQIQLQRLNDYGKVRGQVVIGDDPDNQKVLLNVFPTSAYE
                     KEAKQIIATQQQFYPESLTDKFTEEYCQILTRKRDYFVGPGNEKSRTDYGIYKTDGRT
                     LDNLFEELIGHDKIYPEELRASAASYTAQLFNVLNDLNNLRILNYEDGKLTKEDKEKI
                     IAEIKNNTTTINMLNVIKKVAGCSKDDIKGFRVNEKDKPEISSMPVYRKIHKDLLKAG
                     VDISDWPVEFIDELSFILTLNTENGEIRKQLNNRLAPKFDFLNADLIQLIIDNKDSFE
                     IKTNNKWHRFSVKTMNKLIPEMMERPVEQMTLLNEMGLVKKDKKRFENNKYLPYKEIA
                     KDIFNPVASKSVREALKIVNAVLKKYGHIDYLVVEMPRDKNLKEEQDNIKEFQNKNKK
                     AKDAAFEAFVKSVGSEQRVKEALSKNRKLQMKMRLWYQQQEIDPYNGKTIDATDLINN
                     PDKFEIDHIIPQSISFYDSINNKTLCFASMNQVKGQKTPYEFMLEGHGQSYDKFKATV
                     MANKNFGKAKRANYLFEENVSDIETRKRFLSRNLVDTRYSSRVVLNSLQDFFREKSAD
                     TKVTVIRGKFTSNMRKHWHIDKTRETFHHHAIDASIIAATPFLRMWKKGGTIFPVKVG
                     EESIDIETGEILDDKNFDKAMYEEPYSGFVSEIMNADDRIKFSHQVDKKMNRKVSDAT
                     IYSTRTGKLAKDKKDAEYIVAKVKDIYSVDGFKKFKKVYDKDKTKFLLYKYDPRTFSK
                     LERIISDCPDKVEKVQTNGKVKAVDISPFEMYRRDHGMIKKYSKKGNGPAIKQLKYLD
                     KKLGSHIDITPANANGKHVILQSLKPWRTDVYLNHETGEYEIMGIKYSDLKFNKNEGY
                     GIKKDKYLEIKKVEEVSEKSEFMFSLYRKDRVKVQDMKTGESVELLFWSRNFSNKKYA
                     ELKPISQAENDKKLPVYGKGRLIKRLIPKNCKIWKVNTTILGDPYYLEKESDSPKDIL
                     D*"
     CDS             complement(2464..2976)
                     /label="ORF frame 3"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     misc_feature    complement(5298..5315)
                     /label="Sp6_primer"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     misc_feature    5320..5344
                     /label="attB2"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     terminator      5356..5822
                     /label="hGH_PA_terminator"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     terminator      5828..5863
                     /label="T7_terminator"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     rep_origin      5980..6286
                     /label="f1_origin"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     misc_feature    complement(6400..6420)
                     /label="pBABE_3_primer"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     misc_feature    complement(6406..6621)
                     /label="SV40_enhancer"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     promoter        6418..6686
                     /label="SV40_promoter"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     rep_origin      6585..6662
                     /label="SV40_origin"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     misc_feature    6647..6666
                     /label="SV40pro_F_primer"
                     /translation="MNFAVHRHGADFGFILLIHSESFDIVLGTPGDFLNHIQHVDSCG
                     VVLNFSDDLFLVLLCQFPILVVQDSQIVQVIQHVKQLGGITGCRCPQFLGVDLIVADQ
                     FLEQIVQGSSISLVDPVVGPAFLVSWPHKIISLASQDLAVFLGELVGQALRIELLLSG
                     NDLLCFFLVS*"
     CDS             6801..7595
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6804..7592
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7110..7646)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7772..7891
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7860..7879
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8082..8117
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8140..8157)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8147..8224
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8254..8871)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8851..8884
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9652..10512)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9652..10512)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatggcc gaccgaatct ctctggggct ggacattggg
 1921 gtggcaagcg tggggttctc agtgctggac attgataagg gaaaagtcat tgagctgggc
 1981 gccaggctgt tctctgctac tgtggccgct ggcaaccagg atcgaagaga catgcgagga
 2041 gccaggcgcc tgctgaaccg gaacaagcag cgacggcagg ataccggaaa gctgttcaag
 2101 aaatttggcc tgatcgacga ttttgataag ggcagcttct acgacaactt taatcagaac
 2161 ctgaatcctt atgagctgag agtgaaaggc ctgacagaac agctgactaa ggaggaactg
 2221 gccgagtctc tgtaccagat cgtgaaacat agggggatta gttatgcact gaaggacgcc
 2281 gatgtggacg aaggcgggac agactactca gtcagcctga aaatcaacag ccaggagctg
 2341 gcagaaaaga ctccagccca gattcagctg cagagactga atgattatgg aaaggtgagg
 2401 ggccaggtgg tcatcggcga cgatccagac aaccagaagg tgctgctgaa tgtgttcccc
 2461 acatcagctt acgagaaaga agcaaagcag atcattgcca ctcagcagca gttctatcct
 2521 gagagcctga ccgacaagtt caccgaggaa tactgccaga tcctgactcg caagcgagat
 2581 tattttgtgg ggccaggaaa cgagaaaagc cggaccgact acgggatcta caagactgat
 2641 ggaagaaccc tggacaatct gttcgaggaa ctgatcggcc acgataagat ctaccccgag
 2701 gaactgcggg catctgcagc cagttatacc gcccagctgt ttaacgtgct gaatgacctg
 2761 aacaatctga gaatcctgaa ctacgaggat gggaaactga caaaggagga caaggaaaag
 2821 atcatcgctg aaattaagaa caacaccaca actatcaaca tgctgaatgt gattaagaaa
 2881 gtcgccgggt gttccaagga cgatatcaaa ggattccgag tgaatgagaa ggataaaccc
 2941 gaaatcagct ccatgcctgt gtaccgcaaa attcataagg acctgctgaa ggccggcgtg
 3001 gatatctcag actggcccgt cgagttcatc gacgaactga gctttattct gacactgaac
 3061 actgagaatg gggaaattcg caaacagctg aacaatcgac tggcccctaa gttcgatttt
 3121 ctgaacgctg acctgatcca gctgatcatt gataataagg actcctttga gattaagact
 3181 aacaacaagt ggcacagatt cagcgtgaaa accatgaaca aactgatccc agagatgatg
 3241 gaaagacccg tggagcagat gaccctgctg aatgaaatgg gactggtcaa gaaagataag
 3301 aaacgctttg agaacaataa gtacctgcct tacaaggaaa tcgcaaagga cattttcaac
 3361 ccagtggcct ccaaatctgt ccgcgaggcc ctgaagatcg tgaatgctgt cctgaagaaa
 3421 tacggccaca ttgattatct ggtggtcgaa atgcctcggg ataaaaacct gaaggaggaa
 3481 caggacaata tcaaggagtt ccagaacaaa aataagaaag ctaaggacgc tgcattcgaa
 3541 gcatttgtga aatcagtcgg gagcgagcag agagtgaagg aagccctgtc taaaaaccgg
 3601 aagctgcaga tgaagatgag actgtggtat cagcagcagg agatcgatcc atataatgga
 3661 aagacaatcg atgccactga cctgattaac aatcctgata agttcgagat tgaccatatc
 3721 attccacaga gtatctcatt ttacgacagt attaacaata agaccctgtg cttcgcctca
 3781 atgaaccagg tgaaaggaca gaaaaccccc tacgagttta tgctggaagg ccacgggcag
 3841 tcctatgaca agttcaaagc tacagtgatg gcaaacaaga attttggcaa ggctaaaagg
 3901 gcaaactacc tgttcgagga aaatgtgagc gatatcgaga ctcggaagag attcctgtcc
 3961 cgcaacctgg tggacacccg atattctagt cgggtggtgc tgaacagcct gcaggatttc
 4021 tttcgggaga aatctgccga caccaaggtg acagtcattc gcggcaagtt tacctccaac
 4081 atgcgaaaac attggcacat cgataagact agggagacat tccaccatca cgccattgac
 4141 gcttctatca ttgccgctac accatttctg cgcatgtgga agaaaggagg cactatcttc
 4201 cccgtgaagg tcggagaaga aagtatcgat attgagacag gcgaaattct ggacgataag
 4261 aattttgaca aagcaatgta cgaggaaccc tatagtggct tcgtgtcaga gatcatgaac
 4321 gccgacgatc ggatcaagtt cagccaccag gtggataaga aaatgaatag gaaggtgagc
 4381 gacgccacca tctacagtac tcgcaccggg aaactggcta aggataagaa agacgctgag
 4441 tacatcgtgg caaaggtcaa agatatctac agcgtggacg gattcaagaa gttcaagaaa
 4501 gtctacgata aggacaaaac caagtttctg ctgtacaaat atgatcctag gacattctca
 4561 aagctggagc gcatcattag cgattgccca gacaaagtgg aaaaggtcca gacaaacggc
 4621 aaagtgaagg ctgtcgatat cagtccattc gagatgtaca gaagggacca tgggatgatc
 4681 aagaaatact caaagaaagg aaacggcccc gccatcaagc agctgaagta cctggataag
 4741 aaactgggca gccacatcga cattaccccc gcaaacgcca atggaaaaca cgtgatcctg
 4801 cagagtctga agccttggag aaccgacgtc tatctgaacc acgagacagg cgagtacgaa
 4861 atcatgggga ttaagtatag cgatctgaag ttcaacaaga atgaggggta cggaatcaag
 4921 aaagacaagt atctggaaat taagaaagtg gaggaagtct ccgagaagtc tgagttcatg
 4981 tttagcctgt acaggaagga tcgcgtgaaa gtccaggaca tgaaaaccgg cgagtccgtg
 5041 gaactgctgt tctggagcag gaacttttcc aataagaaat acgctgagct gaagcccatc
 5101 tcccaggcag aaaacgacaa gaaactgcct gtgtatggca aagggagact gatcaagagg
 5161 ctgattccca aaaactgtaa gatctggaaa gtgaatacca caattctggg cgatccctac
 5221 tatctggaga aagaaagcga ctcccctaag gatatcctgg actaagaatt cggtaccgag
 5281 ctcaagcttg cggccgccta tagtgtcacc taaatcgtaa cccagctttc ttgtacaaag
 5341 tggtcgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
 5401 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
 5461 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
 5521 tgacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
 5581 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
 5641 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
 5701 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
 5761 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
 5821 ttctagcata accccttggg gcctctaaac gggtcttgag gggttttttg tctagaagac
 5881 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
 5941 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
 6001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
 6061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
 6121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
 6181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
 6241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
 6301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
 6361 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
 6421 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
 6481 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
 6541 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
 6601 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
 6661 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
 6721 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
 6781 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
 6841 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
 6901 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
 6961 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
 7021 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
 7081 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
 7141 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
 7201 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
 7261 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
 7321 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
 7381 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
 7441 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
 7501 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
 7561 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
 7621 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
 7681 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
 7741 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
 7801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
 7861 tggtttgtcc aaactcatca atgtatctta tcatgtctgt tcatgaagcc ccttgagcat
 7921 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
 7981 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
 8041 ttggtttaga gtttggcaac atatgcccat atgtaactag cataacccct tggggcctct
 8101 aaacgggtct tgaggggttt tttgctgaaa gcatgcggag gaaattctcc ttgaagtttc
 8161 cctggtgttc aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta
 8221 ttaaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
 8281 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
 8341 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
 8401 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
 8461 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
 8521 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
 8581 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
 8641 actcgacgta aacacgttaa atagagcttg gacatattta acatcgggcg tgttagcttt
 8701 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
 8761 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
 8821 tgtagttgag ctttttggaa ttgcgatcgc ataacttcgt atagcataca ttatacgaag
 8881 ttataagctc ggaacgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
 8941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
 9001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
 9061 gctccgcccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
 9121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
 9181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
 9241 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
 9301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
 9361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
 9421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
 9481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
 9541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
 9601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gttaccaatg
 9661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
 9721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
 9781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
 9841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
 9901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
 9961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
10021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
10081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
10141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
10201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
10261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
10321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
10381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
10441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
10501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
10561 catgtccgcg cgtt
//

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