| 质粒名称: | tet-pLKO.puro_shGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 110421 |
| 存储实验室: | Sandra Martha Gomes Dias |
|
载体骨架基本信息 |
|
| 载体名称: | Tet-pLKO-puro |
| 载体出品公司: | #21915 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8816bp |
| 完整质粒大小: | 10633 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | tet-pLKO.puro_shGFP was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110421 ; http://n2t.net/addgene:110421 ; RRID:Addgene_110421) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS tet-pLKO.puro_shGFP 8816 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION shGFP control, silence GFP gene, doxycycline inducible, puromycin
selection.
ACCESSION .
VERSION .
KEYWORDS tet-pLKO.puro_shGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8816)
TITLE Dias lab diverse plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8816)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8816
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..227
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 228..408
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 455..580
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1073..1306
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
primer_bind 1835..1854
/label=H1
/note="Human H1 promoter, forward primer"
protein_bind 1872..1890
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1900..1918
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
misc_feature 2019..2136
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2185..2695
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2614..2632
/label=hPGK-F
/note="Human PGK promoter, forward primer"
intron 2704..3276
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 3284..3303
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
promoter 3331..3349
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3360..3977
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(3433..3452)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
misc_feature 4015..4588
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4182..4199)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4409..4428
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4608..5207
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4608..4627)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5104..5124
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5335..5568
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5640..5761
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5677..5696)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5731..5750
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5801..5936
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5863..5882
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter complement(5957..5975)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5985..6002)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5985..6001)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5994..6016)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6143..6598
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6230..6249)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6440..6461
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6624..6728
/gene="bla"
/label=AmpR promoter
CDS 6729..7589
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6947..6966)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7760..8348
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8249..8268
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8502..8519
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8636..8657
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8672..8702
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8710..8726
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8715..8737
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8734..8750
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8734..8750
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8769..8789
/label=T3
/note="T3 promoter, forward primer"
promoter 8771..8789
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
1801 gagactagcc tcgagcggcc gcaatatttg catgtcgcta tgtgttctgg gaaatcacca
1861 taaacgtgaa atccctatca gtgatagaga cttataagtt ccctatcagt gatagagaca
1921 ccggcaagct gaccctgaag ttcatctcga gatgaacttc agggtcagct tgtttttgaa
1981 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
2041 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
2101 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
2161 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg
2221 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
2281 tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
2341 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
2401 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
2461 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
2521 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
2581 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
2641 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga
2701 tctgtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta
2761 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac
2821 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct
2881 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa
2941 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt
3001 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt
3061 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat
3121 attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta
3181 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct
3241 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
3301 gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg cgaattgata
3361 tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
3421 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
3481 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
3541 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
3601 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
3661 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
3721 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
3781 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
3841 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
3901 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
3961 ttaaatgtga aagtgggtcc gcgtacagcg gctcccggga gttctaggga tctgcccctc
4021 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
4081 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
4141 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
4201 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
4261 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
4321 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
4381 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
4441 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
4501 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
4561 ggttttcctt tgaaaaacac gatgataagg atccaccgga gcttaccatg accgagtaca
4621 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
4681 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
4741 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
4801 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
4861 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
4921 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
4981 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
5041 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
5101 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
5161 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
5221 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct ttaagaccaa
5281 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
5341 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
5401 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
5461 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
5521 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
5581 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
5641 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
5701 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
5761 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
5821 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
5881 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
5941 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
6001 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
6061 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
6121 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
6181 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
6241 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
6301 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
6361 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
6421 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
6481 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
6541 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
6601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
6661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
6721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
6781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
6841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
6901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
6961 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
7021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
7081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
7141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
7201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
7261 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
7321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
7381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
7441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
7501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
7561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
7621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
7681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
7741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
7801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
7861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
7921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
7981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
8041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
8101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
8161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
8221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
8281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
8341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
8401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
8461 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
8521 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
8581 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
8641 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
8701 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
8761 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
