| 质粒名称: | pcDNA AXL |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 105932 |
| 存储实验室: | Rosa Marina Melillo |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA4/TO/myc/HIS |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 7848 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | AXL |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 951 bp |
| GenBank编号 | AAH32229.1 |
| 启动子: | tet-inducible CMV |
| 标签/融合蛋白: |
myc (C terminal on backbone)
His (C terminal on backbone)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (unknown if destroyed) |
| 3’克隆位点: | EcoRI (unknown if destroyed) |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA AXL was a gift from Rosa Marina Melillo (Addgene plasmid # 105932 ; http://n2t.net/addgene:105932 ; RRID:Addgene_105932) |
| 参考文献部分: | Molecular mechanism of 17-allylamino-17-demethoxygeldanamycin (17-AAG)-induced AXL receptor tyrosine kinase degradation. Krishnamoorthy GP, Guida T, Alfano L, Avilla E, Santoro M, Carlomagno F, Melillo RM. J Biol Chem. 2013 Jun 14;288(24):17481-94. doi: 10.1074/jbc.M112.439422. Epub 2013 Apr 29. 10.1074/jbc.M112.439422 PubMed 23629654 |
买家导航
pcDNA AXL载体质粒基本信息
pcDNA AXL质粒图谱载体图谱和pcDNA AXL载体序列质粒序列多克隆位点信息
pcDNA AXL质粒载体简介
pcDNA AXL质粒序列载体序列
LOCUS pcDNA AXL 7848 bp DNA circular SYN 25-AUG-2024
DEFINITION Mammalian expression of myc-His tagged AXL.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7848)
AUTHORS Krishnamoorthy GP, Guida T, Alfano L, Avilla E, Santoro M,
Carlomagno F, Melillo RM
TITLE Molecular mechanism of 17-allylamino-17-demethoxygeldanamycin
(17-AAG)-induced AXL receptor tyrosine kinase degradation.
JOURNAL J Biol Chem. 2013 Jun 14;288(24):17481-94. doi:
10.1074/jbc.M112.439422. Epub 2013 Apr 29.
PUBMED 23629654
REFERENCE 2 (bases 1 to 7848)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1074/jbc.M112.439422"; journalName: "J Biol Chem"; date:
"2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17481-94"
FEATURES Location/Qualifiers
source 1..7848
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 170..549
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 550..753
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 704..724
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 755..773
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 776..794
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 804..828
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3706..3735
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3751..3768
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(3791..3808)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3797..4021
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4067..4495
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4154..4173)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4364..4385
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4504..4524)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4509..4838
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4689..4824
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4751..4770
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 4886..4933
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 4952..5326
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 5456..5577
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5493..5512)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5547..5566
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5626..5642)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5626..5642)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5639..5661)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5650..5666
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5674..5704)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5719..5740
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5857..5874)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6028..6616)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6108..6127)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6787..7647)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7410..7429
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7648..7752)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7827..7846)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
61 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
121 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
181 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
241 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
301 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
361 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
421 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
481 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
541 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
601 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
661 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
721 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
781 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
841 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
901 gcgtttaaac ttaagcttgg taccgagctc ggatccagcc caacaacttc tgaggaaagt
961 ttggcaccca tggcgtggcg gtgccccagg atgggcaggg tcccgctggc ctggtgcttg
1021 gcgctgtgcg gctgggcgtg catggccccc aggggcacgc aggctgaaga aagtcccttc
1081 gtgggcaacc cagggaatat cacaggtgcc cggggactca cgggcaccct tcggtgtcag
1141 ctccaggttc agggagagcc ccccgaggta cattggcttc gggatggaca gatcctggag
1201 ctcgcggaca gcacccagac ccaggtgccc ctgggtgagg atgaacagga tgactggata
1261 gtggtcagcc agctcagaat cacctccctg cagctttccg acacgggaca gtaccagtgt
1321 ttggtgtttc tgggacatca gaccttcgtg tcccagcctg gctatgttgg gctggagggc
1381 ttgccttact tcctggagga gcccgaagac aggactgtgg ccgccaacac ccccttcaac
1441 ctgagctgcc aagctcaggg acccccagag cccgtggacc tactctggct ccaggatgct
1501 gtccccctgg ccacggctcc aggtcacggc ccccagcgca gcctgcatgt tccagggctg
1561 aacaagacat cctctttctc ctgcgaagcc cataacgcca agggggtcac cacatcccgc
1621 acagccacca tcacagtgct cccccagcag ccccgtaacc tccacctggt ctcccgccaa
1681 cccacggagc tggaggtggc ttggactcca ggcctgagcg gcatctaccc cctgacccac
1741 tgcaccctgc aggctgtgct gtcagacgat gggatgggca tccaggcggg agaaccagac
1801 cccccagagg agcccctcac ctcgcaagca tccgtgcccc cccatcagct tcggctaggc
1861 agcctccatc ctcacacccc ttatcacatc cgcgtggcat gcaccagcag ccagggcccc
1921 tcatcctgga cccactggct tcctgtggag acgccggagg gagtgcccct gggcccccct
1981 gagaacatta gtgctacgcg gaatgggagc caggccttcg tgcattggca agagccccgg
2041 gcgcccctgc agggtaccct gttagggtac cggctggcgt atcaaggcca ggacacccca
2101 gaggtgctaa tggacatagg gctaaggcaa gaggtgaccc tggagctgca gggggacggg
2161 tctgtgtcca atctgacagt gtgtgtggca gcctacactg ctgctgggga tggaccctgg
2221 agcctcccag tacccctgga ggcctggcgc ccagggcaag cacagccagt ccaccagctg
2281 gtgaaggaac cttcaactcc tgccttctcg tggccctggt ggtatgtact gctaggagca
2341 gtcgtggccg ctgcctgtgt cctcatcttg gctctcttcc ttgtccaccg gcgaaagaag
2401 gagacccgtt atggagaagt gtttgaacca acagtggaaa gaggtgaact ggtagtcagg
2461 taccgcgtgc gcaagtccta cagtcgtcgg accactgaag ctaccttgaa cagcttgggc
2521 atcagtgaag agctgaagga gaagctgcgg gatgtgatgg tggaccggca caaggtggcc
2581 ctggggaaga ctctgggaga gggagagttt ggagctgtga tggaaggcca gctcaaccag
2641 gacgactcca tcctcaaggt ggctgtgaag acgatgaaga ttgccatctg cacgaggtca
2701 gagctggagg atttcctgag tgaagcggtc tgcatgaagg aatttgacca tcccaacgtc
2761 atgaggctca tcggtgtctg tttccagggt tctgaacgag agagcttccc agcacctgtg
2821 gtcatcttac ctttcatgaa acatggagac ctacacagct tcctcctcta ttcccggctc
2881 ggggaccagc cagtgtacct gcccactcag atgctagtga agttcatggc agacatcgcc
2941 agtggcatgg agtatctgag taccaagaga ttcatacacc gggacctggc ggccaggaac
3001 tgcatgctga atgagaacat gtccgtgtgt gtggcggact tcgggctctc caagaagatc
3061 tacaatgggg actactaccg ccagggacgt atcgccaaga tgccagtcaa gtggattgcc
3121 attgagagtc tagctgaccg tgtctacacc agcaagagcg atgtgtggtc cttcggggtg
3181 acaatgtggg agattgccac aagaggccaa accccatatc cgggcgtgga gaacagcgag
3241 atttatgact atctgcgccg gggaaatcgc ctgaagcagc ctgcggactg tctggatgga
3301 ctgtatgcct tgatgtcgcg gtgctgggag ctaaatcccc aggaccggcc aagttttaca
3361 gagctgcggg aagatttgga gaacacactg aaggccttgc ctcctgccca ggagcctgac
3421 gaaatcctct atgtcaacat ggatgagggt ggaggttatc ctgaaccccc tggagctgca
3481 ggaggagctg accccccaac ccagccagac cctaaggatt cctgtagctg cctcactgcg
3541 gctgaggtcc atcctgctgg acgctatgtc ctctgccctt ccacaacccc tagccccgct
3601 cagcctgctg ataggggctc cccagcagcc ccagggcagg aggatggtgc cgaattctgc
3661 agatatccag cacagtggcg gccgctcgag tctagagggc ccttcgaaca aaaactcatc
3721 tcagaagagg atctgaatat gcataccggt catcatcacc atcaccattg agtttaaacc
3781 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
3841 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
3901 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
3961 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
4021 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
4081 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
4141 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
4201 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
4261 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
4321 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4381 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
4441 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
4501 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
4561 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
4681 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
4741 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
4801 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
4861 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
4921 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
4981 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
5041 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
5101 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
5161 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
5221 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
5281 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
5341 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
5401 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
5461 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
5521 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
5581 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
5641 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
5701 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
5761 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
5821 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
7081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
7381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
7801 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgca
//
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