| 质粒名称: | pZW1-snoVector |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 73174 |
| 存储实验室: | Ling-Ling Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pZW1 |
| 载体出品公司: | Zefeng Wang's lab |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SNORD116-13,HBII-85-13 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 94 bp |
| 基因突变: | -- |
| GenBank编号 | 100033425 |
| 启动子 | CMV promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI（克隆过程中销毁） |
| 3' 克隆位点: | SacII（克隆过程中销毁） |
| 5' 测序引物 | EGFP-Seq-5F: GACCACATGAAGCAGCACGA |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pZW1-snoVector was a gift from Ling-Ling Chen (Addgene plasmid # 73174 ; http://n2t.net/addgene:73174 ; RRID:Addgene_73174) |
| 参考文献部分: | SnoVectors for nuclear expression of RNA. Yin QF, Hu SB, Xu YF, Yang L, Carmichael GG, Chen LL. Nucleic Acids Res. 2015 Jan;43(1):e5. doi: 10.1093/nar/gku1050. Epub 2014 Nov 5. 10.1093/nar/gku1050 PubMed 25378317 |
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pZW1-snoVector载体 pZW1snoVector质粒基本信息
pZW1-snoVector质粒图谱 pZW1snoVector载体图谱和pZW1-snoVector载体序列 pZW1snoVector质粒序列多克隆位点信息
pZW1-snoVector质粒 pZW1snoVector载体简介
pZW1-snoVector质粒序列 pZW1snoVector载体序列
LOCUS pZW1-snoVector 4977 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Nuclear expression of RNAs.
ACCESSION .
VERSION .
KEYWORDS pZW1-snoVector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4977)
AUTHORS Yin QF, Hu SB, Xu YF, Yang L, Carmichael GG, Chen LL
TITLE SnoVectors for nuclear expression of RNA.
JOURNAL Nucleic Acids Res. 2015 Jan;43(1):e5. doi: 10.1093/nar/gku1050. Epub
2014 Nov 5.
PUBMED 25378317
REFERENCE 2 (bases 1 to 4977)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4977
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 607..616
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(658..679)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1222..1241)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1569..1590
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1765..1886
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1802..1821)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1856..1875
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1893..2348)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2030..2051)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2242..2261
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2375..2479
/gene="bla"
/label=AmpR promoter
promoter 2481..2838
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2504..2524)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2689..2824
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2751..2770
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2873..3667
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2927..2946)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3537..3556
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3855..3874)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3899..3946
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4275..4863
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4764..4783
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggta
901 agtctcgagt gagatcttgg accaatgatg acttccatac atgcattcct tggaaagctg
961 aacaaaatga gtgggaactc tgtactatca tcttagttga actgaggtcc accgggggct
1021 aaagcttcga attctgcagt cgacggtacc aagattgtgt gtggatcgat gatgacttcc
1081 atatatacat tccttggaaa gctgaacaaa atgagtgaaa actctatacc gtcattctcg
1141 tcgaactgag gtccagcaca ttactccaac agttaattaa ccgcggtctc tttcttccag
1201 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
1261 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
1321 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
1381 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
1441 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
1501 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
1561 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
1621 gagctgtaca agtaaggatc caccggatct agataactga tcataatcag ccataccaca
1681 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
1741 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
1801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
1861 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
1921 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
1981 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
2041 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
2101 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
2161 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
2221 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
2281 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
2341 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
2401 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
2461 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
2521 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2581 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2641 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2701 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2761 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2821 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
2881 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
2941 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
3001 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
3061 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
3121 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
3181 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
3241 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
3301 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
3361 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
3421 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
3481 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
3541 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
3601 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
3661 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
3721 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
3781 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
3841 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
3901 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
3961 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
4021 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
4081 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
4141 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
4201 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
4261 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
4321 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
4381 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
4441 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
4501 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
4561 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
4621 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
4681 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
4741 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
4801 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
4861 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
4921 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
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