| 质粒名称: | pX333 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 64073 |
| 存储实验室: | Andrea Ventura |
|
载体骨架基本信息 |
|
| 载体名称: | pX333 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CBh; U6 |
| 标签/融合蛋白: | 3XFLAG-Cas9（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pX333 was a gift from Andrea Ventura (Addgene plasmid # 64073 ; http://n2t.net/addgene:64073 ; RRID:Addgene_64073) |
| 参考文献部分: | In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. 10.1038/nature13902 PubMed 25337876 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pX333 8990 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Vector for tandem expression of two sgRNAs from two independent U6
promoters. Cas9 is expressed by Cbh promoter..
ACCESSION .
VERSION .
KEYWORDS pX333
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8990)
AUTHORS Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC,
Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura
A
TITLE In vivo engineering of oncogenic chromosomal rearrangements with the
CRISPR/Cas9 system.
JOURNAL Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub
2014 Oct 22.
PUBMED 25337876
REFERENCE 2 (bases 1 to 8990)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8990
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 70..310
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 337..412
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 503..743
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 778..853
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 943..1228
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1230..1507
/label=chicken beta-actin promoter
intron 1508..1735
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1747..1756
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1756..1821
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1828..1848
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1873..5973
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5974..6021
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(6049..6066)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6055..6262
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 6271..6411
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 6271..6400
/label=AAV2 ITR
rep_origin 6486..6941
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6573..6592)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6783..6804
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6958..6977)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7077..7099
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7137..7155)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7223..7327
/gene="bla"
/label=AmpR promoter
CDS 7328..8188
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7546..7565)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8359..8947
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8848..8867
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ggccttttgc tcacatgcgc cattctcgag catgcgccat tctcgagcat gctcgagaat
61 ggcgcatgtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
121 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
181 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
241 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
301 tggaaaggac gaaacaccgg gtcttcgaga agacctgttt tagagctaga aatagcaagt
361 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt
421 tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat
481 tctgcagaca aatggctcta gagagggcct atttcccatg attccttcat atttgcatat
541 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
601 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
661 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
721 tttatatatc ttgtggaaag gacgaaacac cggagaccta ttcgccttaa ggtctcggtt
781 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
841 accgagtcgg tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc
901 gtttttagcg cgtgcgccaa ttctgcagac aaatggggta cccgttacat aacttacggt
961 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc
1021 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
1081 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
1141 gcccgcctgg cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat
1201 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
1261 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
1321 tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
1381 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
1441 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
1501 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
1561 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
1621 ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg tttaagggat ggttggttgg
1681 tggggtatta atgtttaatt acctggagca cctgcctgaa atcacttttt ttcaggttgg
1741 accggtgcca ccatggacta taaggaccac gacggagact acaaggatca tgatattgat
1801 tacaaagacg atgacgataa gatggcccca aagaagaagc ggaaggtcgg tatccacgga
1861 gtcccagcag ccgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc
1921 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
1981 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
2041 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
2101 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
2161 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
2221 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
2281 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
2341 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
2401 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
2461 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
2521 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
2581 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
2641 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
2701 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
2761 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
2821 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
2881 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
2941 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
3001 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
3061 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
3121 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
3181 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
3241 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
3301 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
3361 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
3421 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
3481 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
3541 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
3601 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
3661 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
3721 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
3781 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
3841 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
3901 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
3961 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
4021 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
4081 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
4141 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
4201 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
4261 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
4321 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
4381 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
4441 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
4501 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
4561 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
4621 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
4681 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
4741 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
4801 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
4861 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
4921 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
4981 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
5041 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
5101 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
5161 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
5221 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
5281 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
5341 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
5401 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
5461 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
5521 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
5581 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
5641 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
5701 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
5761 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
5821 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
5881 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
5941 acacggatcg acctgtctca gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc
6001 ggccaggcaa aaaagaaaaa gtaagaattc ctagagctcg ctgatcagcc tcgactgtgc
6061 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
6121 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
6181 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
6241 agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc
6301 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
6361 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
6421 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
6481 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
6541 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
6601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
6661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
6721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
6781 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
6841 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
6901 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
6961 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
7021 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
7081 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
7141 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
7201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
7261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
7321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
7381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
7441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
7501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
7561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
7621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
7681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
7741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
7801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
7861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
7921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
7981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
8041 tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
8101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
8161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
8221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
8281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
8341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
8401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
8461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
8521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
8581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
8641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
8701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
8761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
8821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
8881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
8941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pLenti-sgRNA
- 下一篇：pSBbi-GN
