| 质粒名称: | p2lox-cAMPr |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 99143 |
| 存储实验室: | Justin Blau |
|
载体骨架基本信息 |
|
| 载体名称: | p2Lox |
| 载体出品公司: | Michael Kyba |
| 空载体大小: | 2660 bp |
| 完整质粒大小: | 6042 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,Cre/Lox |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | cAMPr,cAMPr, a cp-GFP based sensor for cAMP |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2511 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | cpGFP |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p2lox-cAMPr was a gift from Justin Blau (Addgene plasmid # 99143 ; http://n2t.net/addgene:99143 ; RRID:Addgene_99143) |
| 参考文献部分: | cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Hackley CR, Mazzoni EO, Blau J. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 10.1126/scisignal.aah3738. 10.1126/scisignal.aah3738 PubMed 29511120 |
买家导航
p2lox-cAMPr载体 p2loxcAMPr质粒基本信息
p2lox-cAMPr质粒图谱 p2loxcAMPr载体图谱和p2lox-cAMPr载体序列 p2loxcAMPr质粒序列多克隆位点信息
p2lox-cAMPr质粒 p2loxcAMPr载体简介
p2lox-cAMPr质粒序列 p2loxcAMPr载体序列
LOCUS p2lox-cAMPr 6043 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses cAMPr in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS p2lox-cAMPr
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6043)
AUTHORS Hackley CR, Mazzoni EO, Blau J
TITLE cAMPr: A single-wavelength fluorescent sensor for cyclic AMP.
JOURNAL Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi:
10.1126/scisignal.aah3738.
PUBMED 29511120
REFERENCE 2 (bases 1 to 6043)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6043
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..526
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(62..85)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 459..478
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 561..570
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind 576..609
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 612..645
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
primer_bind 647..663
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 648..664
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1954..1975
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(2978..2994)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2978..2994)
/label=pBluescriptSK
/note="For pBluescript vector"
protein_bind complement(3018..3042)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 3068..3109
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 3119..3136
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
polyA_signal 3163..3284
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3174..3193)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3228..3247
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3467..3922
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3554..3573)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3764..3785
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3989..4008)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4108..4130
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4168..4186)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4254..4358
/gene="bla"
/label=AmpR promoter
CDS 4359..5219
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4577..4596)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5390..5978
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5879..5898
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 acagatctcg acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
61 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
121 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
181 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
241 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
301 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
361 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
421 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
481 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct
541 agatctcgaa ggatctggag gccaccatgg tgtcgataac ttcgtatagc atacattata
601 cgaagttatc gataacttcg tataggatac tttatacgaa gttatctcga ggtcgacggt
661 atcgataagc ttgatatcga attccaccat gggcaacgcc gccgccgcca agaagggcag
721 cgagcaggag agcgtgaaag agttcctagc caaagccaag gaagatttcc tgaaaaaatg
781 ggagacccct tctcagaata cagcccagtt ggatcagttt gatagaatca agacccttgg
841 caccggctcc tttgggcgag tgatgctggt gaagcacaag gagagtggga accactacgc
901 catgaagatc ttagacaagc agaaggtggt gaagctaaag cagatcgagc acactctgaa
961 tgagaagcgc atcctgcagg ccgtcaactt cccgttcctg gtcaaacttg aattctcctt
1021 caaggacaac tcaaacctgt acatggtcat ggagtatgta gctggtggcg agatgttctc
1081 ccacctacgg cggattggaa ggttcagcga gccccatgcc cgtttctacg cggcgcagat
1141 cgtcctgacc tttgagtatc tgcactccct ggacctcatc taccgggacc tgaagcccga
1201 gaatcttctc atcgaccagc agggctatat tcaggtgaca gacttcggtt ttgccaagcg
1261 tgtgaaaggc cgtacttgga ccttgtgtgg gacccctgag tacttggccc ccgagattat
1321 cctgagcaaa ggctacaaca aggctgtgga ctggtgggct ctcggagtcc tcatctacga
1381 gatggctgct ggttacccac ccttcttcgc tgaccagcct atccagatct atgagaaaat
1441 cgtctctggg aaggtgcggt tcccatccca cttcagctct gacttgaagg acctgctgcg
1501 gaaccttctg caagtggatc tcaccaagcg ctttgggaac ctcaagaacg gggtcaatga
1561 catcaagaac cacaagtggt ttgccacgac tgactggatt gccatctatc agagaaaggt
1621 ggaagctccc ttcataccaa agtttaaagg ccctggggac acgagtaact ttgacgacta
1681 tgaggaggaa gagatccggg tctccatcaa tgagaagtgt ggcaaggagt ttactgagtt
1741 tcccgggaac gtctatatca aggccgacga gcagaagaac ggcatcaagg cgtacttcaa
1801 gatccgccac aacatcgagg gcggcggcgt gcagctcgcc taccactacc agcagaacac
1861 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccat
1921 gctttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
1981 cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggag ggagcgaatc
2041 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatccagg tcgagctgga
2101 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta
2161 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
2221 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
2281 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
2341 cttcaagggc gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
2401 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
2461 caagctggag tacaacaccg ccccccccgc atgcaagggc cggcggcgac gcggggccat
2521 cagcgctgag gtctacaccg aggaggatgc cgcgtcctat gttcggaagg ttataccgaa
2581 agattataag acaatggctg ctttagctaa agccattgaa aagaacgtac tgttttcaca
2641 tcttgatgat aacgagagaa gtgacatttt tgacgccatg ttcccggttt cctttattgc
2701 tggagagact gttattcagc agggtgacga aggggataac ttctacgtga ttgaccaagg
2761 agagatggac gtctatgtca acaatgaatg ggcaaccagt gttggggaag gagggagctt
2821 cggggagctt gctctgattt acgggactcc tcgagcggcc actgtcaagg ccaagacgaa
2881 cgtgaaactg tggggcattg accgggacag ctacagaagg atcctcatgg gaagcacgct
2941 gagaaagcgg aagatgtaaa attcctgcag cccgggggat ccactagttc tagagcggcc
3001 gcagggtggg cgcgccgacc cagctttctt gtacaaagtg gttgatctag agggcccgcg
3061 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
3121 tcatcaccat caccattgag cggccgcttc gagcagacat gataagatac attgatgagt
3181 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
3241 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
3301 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
3361 tctacaaatg tggtaaaatc gcgataagga tccgggctgg cgtaatagcg aagaggcccg
3421 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
3481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
3541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
3601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
3661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
3721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
3781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
3841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
3901 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
3961 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
4021 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
4081 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
4141 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
4201 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4261 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4321 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4381 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4441 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4501 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4561 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4621 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4681 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4741 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
4801 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
4861 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
4921 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
4981 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
5041 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
5101 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
5161 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
5221 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5521 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
5761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
5821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
5881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
5941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
6001 tacggttcct ggccttttgc tggccttttg ctcacatggc tcg
//
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