pENTR4-Dual

价格：2000元

联系方式：I47-825O-882O

买家导航

pENTR4-Dual载体质粒基本信息

出品公司:	Invitrogen
载体名称:	pENTR4-Dual
质粒类型:	Gateway载体；入门载体
高拷贝/低拷贝:	高拷贝
启动子:	无
克隆方法:	限制性内切酶；多克隆位点
载体大小:	3757 bp
5' 测序引物及序列:	pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
3' 测序引物及序列:	pENTR-R: ATGGCTCATAACACCCCTTG
载体标签:	无
载体抗性:	卡那霉素和氯霉素
真核筛选标记:	无
备注:	pENTR4-Dual载体本身含有CCDb致死基因，能够使得普通的DH5alpha等细菌死亡，必须使用DB3.1感受态细胞进行质粒扩增；构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
产品目录号:	--
病毒/非病毒:	非病毒

pENTR4-Dual质粒图谱载体图谱和pENTR4-Dual载体序列质粒序列多克隆位点信息

pENTR4-Dual载体图谱

pENTR4-Dual质粒载体简介

pENTR4-Dual质粒序列载体序列

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCATGGATCTCGGGGACGTCTAA
CTACTAAGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTT
TCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAC
GTTGTGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAACTAAGC
AGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTTAGTTAGTTACTTAAGCT
CGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGC
TTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCACCATGGGAACCAATTCAGTCGACTGGATCC
GGTACCGAATTCGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGT
GGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGG
ATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAA
TGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACA
AGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAAT
GAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAA
ACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATG
TGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGC
CAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTT
TTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATG
CCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGC
GGGGCGTAATCTAGAGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTT
GCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGT
ATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAA
GGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATA
TTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCC
CCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGT
GTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCA
TTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACGAAT
TCGCGGCCGCACTCGAGATATCTAGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCT
TATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGCAGC
TCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAA
ACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCG
CGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAG
GTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAG
CGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACC
ATCAAGCATTTTATCCGTACTCCTGGTGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCAT
TCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCG
GTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAA
TCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAAC
AAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTC
ACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCA
GACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGC
TTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTT
TTTCTAATCAGAATTGGTTAATTGGTTGTAACATTATTCAGATTGGGCCCCGTTCCACTGAGCGTCAGAC
CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA
AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC
TGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAG
AACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA
AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG
GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTA
TGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG
GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCT
CTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG
GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

pEYFP-Mito	pZsGreen1-DR
pZL507	pLpp-STII
FUGW	pCDFDuet-1
pHS24	pGEX4T-1-TEV
pcDNA6/TR	pCMV-Gluc
pESP-1	pEF6/His A
pENTR11-Dual	pBT-2
pPZP121	pGL2-Basic
p426 GAL	plRES2-ZsGreen1
pEarleyGate301	pBMT-2
pGro7	pTagBFP-N
pcDNA6.2/EmGFP-Bsd/V5-DEST	pNZ8048
pCas	pSAT6 nCerulean C(A+)
pCMV-HA-SRC	pDC316-ZsGreen1-shRNA
pFUSE-hIgG1e2-Fc2	pIF413
pNTAP-Shuttle-A	pGR106
pcDNA3.1/His C	pGluc-basic
pBR322	pDdGal-16
pCA4	pS-hydro_TA
pLenti6.3-MCS-IRES2-EGFP	pT2-Venus
pJG4-5	pPIC6αB
pEA300	pSUPER.neo+gfp
ptdTomato	FUW-tetO-hKLF4
pET-41 EK/LIC	pYC50
pNAD_TA	pCL3
pSilencer 3.0-H1	pGL4.29
pBMT-3	pIJ702-87LEdel(78-86)
pTRV2-Gateway	pDEST32
pIP501	pIRES2-AcGFP1-Nuc
pBARGPE1-EGFP	pNFkB-DD-tdTomato
pcDNA3.1+P2A-eGFP	pGuide-IT-ZsGreen1
pGL4.16[luc2CP/Hygro]	pMSCVhyg
pOrf681_TA	pBTL-2
pHR40	pMHE5
pC221	pJHAM005
pEXT-20	pBARMTE1
pET-21c(+)	pAG6O
pCMV5	PLPP-ompA
pCambia1281Z	pCMV6-AC-GFP
pNL4-3-Luc-R-E-	Ptrg-GAL11P
pBR325	pTALEN_v2 (NN)
pET-15b	ptac12
pcDNA3.1/Myc-His C	pAAV-IRES-tdTomato
pYCP211	pKT240
pET-39b(+)	pEYFP-actin
pCas-Scramble-EF1a-GFP	pTALEN_v2 (NI)
pJB8	pLJ3
pCTAP-Shuttle-B	pGWB21
pNC2	pMA5
pGL4.14[luc2/Hygro]	pAS32-S

pcDNA3-HA-mRipk3	pCS-kI
ERM-APEX2	pWS158
pcDNA-Ikka-HA WT	pAAV.CMV.PI.EGFP.WPRE.bGH
pL-CRISPR.EFS.GFP	pFGC-pcoCas9
ORFtag_TetR-SD_Frame1	pCDH-EF1
pAAV2/2	pMCSG7-Wt-NmeCas9
pCpf1-sp	pRB897
M50 Super 8x TOPFlash	pKM208
PB-TRE-dCas9-KRAB-MeCP2	pBABEhygroHigheGFPhTERT
pRLG3A	pQCascade-IS3
pT7-NSP4SA11	pSECC-sgTom
12xMoonTag-12xSunTag-kif18b-24xPP7	pEG302 22aa SunTag NtDRMcd (no NLS) SUP
pNW55	pOBCol3.6-GFPtpz
pJL1-sfGFP	MIP 247 CoMiP 4in1 with shRNA p53
pTAL1	pSBbi-Pur
pTetR-LasR-pLuxR-GFP	pBABE-puro mCherry-EGFP-LC3B
pcDNA3-ExRai-AMPKAR	pEGFP-VSVG
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mChe	pCutamp
pJH2971	pET_His6-SUMO-TEV-TtCsm6
phOct4-EGFP	pSGKP-km
pBabe EGFR(L858R/T790M)	pcDNA3-Barkor 1-180
pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)	pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + S
phCMV-GALV-MTR	pLVX-puro_G-CEPIA1er
pLV-EF1a-V5-Sec61B	pCL-MFG-BRCA1
pEF-Bos MAL Flag	pEBTet_NADP-Snifit-cyto
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK	pHaloTag-EGFP
pGTMV	pKC-4.04
pCX-ThPDE5VV	pGL3 BRE Luciferase
pBTR(hCc)	pcDNA3/HA-FLAG RBP2 H483A
MSP1673	pHR-SFFV-dCas9-BFP
pDEST40-2XFL-wtSrc	FUW-tetO-EGFP
pJBEI-6410	pTE-10M0X
pMyosin-PSmOrange	pSLIK-Neo
MXS_BidirectionalCAG	pDae079 (eMutaT7[transition]
pFUS_A	pRK5-p18-HA
PX853	pscALPSpuro-RsACE2
HA-AR-LBD	pcDNA3-ExRai-AMPKAR(T/A)
pMSCV-LTR-dCas9-VP64-BFP	rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR
pBabepuro-KIBRA	mEGFP-Lifeact-7
pT2/shp53/GFP4	pcDNA3.1-hAsCpf1(TYCV) (pY210)
FUW-M2rtTA	pFE-sfGFP
pDEST47-MCU-GFP	pET His6 MBP TEV LIC cloning vector (1M)
pRPF215	At2S3:RUBY
pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L13	pJL-TRBO-G
pHAGE2-TetOminiCMV-SKM	MSP469
pRH2521	pSLIK-IDH2-R172K-FLAG
pLVX-EF1a-tetOn-IRES-G418 (EtO)	GFP-ING2(pHD)
pHPRT-DRGFP	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
P336-FBP_11	pENTR5'_ubi:loxP-EGFP-loxP
pBAD-bARGSer-AxeTxe	pCAG-VSFP Butterfly 1.2

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pENTR4-Dual

买家导航

pENTR4-Dual载体质粒基本信息

pENTR4-Dual质粒图谱载体图谱和pENTR4-Dual载体序列质粒序列多克隆位点信息

pENTR4-Dual质粒载体简介

pENTR4-Dual质粒序列载体序列

pENTR4-Dual载体图谱质粒图谱pdf版和pENTR4-Dual载体序列质粒序列等相关资料下载

pENTR4-Dual质粒载体应用举例

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