pSico

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司:  
载体名称: pSico
质粒类型: 哺乳动物细胞慢病毒表达载体,RNAi, Cre/loxP
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 7566 bp
5' 测序引物及序列: EGFP-CCATGGTCCTGCTGGAGTTCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄
备注: pSico载体表达EGFP标记, 但是这个绿色荧光蛋白不是融合性的表达蛋白. Cre酶使得EGFP能够能够从载体中切除出来,同时激活shRNA的表达。
产品目录号:  
稳定性: /
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pSico载体图谱


载体简介

pSico载体允许条件控制(通过Cre-LoxP),稳定表达shRNAs,能够RNA干扰细胞和转基因小鼠的基因表达。Cre序列的加入能够使shRNA表达。

载体序列

LOCUS       pSico	7566 bp 	DNA     circular	SYN
DEFINITION  pSico
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="pSico"
                     /mol_type="other DNA"
     promoter        239..815
                     /label="CMV_immearly_promoter"
     misc_feature    318..605
                     /label="CAG_enhancer"
     misc_feature    772..792
                     /label="CMV_fwd_primer"
     misc_feature    835..1015
                     /label="truncHIV-1_3_LTR"
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
     CDS             1564..2451
                     /label="ORF frame 1"
     misc_feature    1686..1919
                     /label="RRE"
     misc_feature    2450..2465
                     /label="cPPT"
     misc_feature    2892..2925
                     /label="loxP"
     misc_feature    complement(2892..2925)
                     /label="loxP"
     promoter        2974..3526
                     /label="CMV_immearly_promoter"
     misc_feature    3029..3316
                     /label="CAG_enhancer"
     misc_feature    3483..3503
                     /label="CMV_fwd_primer"
     promoter        3484..3553
                     /label="CMV_promoter"
     CDS             complement(3562..4305)
                     /label="ORF frame 1"
     CDS             3577..4296
                     /label="ORF frame 1"
     gene            3580..4293
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3622..3643)
                     /label="EGFP_N_primer"
     misc_feature    3760..3789
                     /label="Y66 (EGFP)"
     misc_feature    4230..4251
                     /label="EGFP_C_primer"
     misc_feature    4304..4337
                     /label="loxP"
     misc_feature    4363..4379
                     /label="pBluescriptKS_primer"
     misc_feature    4457..5044
                     /label="WPRE"
     CDS             4545..5099
                     /label="ORF frame 3"
     CDS             4558..5598
                     /label="ORF frame 1"
     misc_feature    complement(5047..5063)
                     /label="pBluescriptKS_primer"
     misc_feature    5235..5250
                     /label="cPPT"
     misc_feature    5235..5256
                     /label="U3PPT"
     misc_feature    5572..5752
                     /label="HIV-1_5_LTR"
     misc_feature    5572..5752
                     /label="truncHIV-1_3_LTR"
     gene            complement(6573..7433)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(6573..7433)
                     /label="ORF frame 2"
     promoter        complement(7475..7503)
                     /label="AmpR_promoter"
ORIGIN
    1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
   61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
  121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
  181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
  241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
  301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
  361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
  421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
  481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
  541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
  601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
  661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
  721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
  781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
  841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
  901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
  961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
 1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
 1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
 1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
 1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
 1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
 1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
 1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
 1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
 1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG GCCGCGCTGA TCTTCAGACC TGGAGGAGGA
 1561 GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA
 1621 TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG
 1681 GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG
 1741 TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC
 1801 AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC
 1861 AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG
 1921 GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT
 1981 TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA
 2041 GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA
 2101 GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT
 2161 AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA
 2221 GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA
 2281 CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA
 2341 GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCGGCA
 2401 CTGCGTGCGC CAATTCTGCA GACAAATGGC AGTATTCATC CACAATTTTA AAAGAAAAGG
 2461 GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC ATAATAGCAA CAGACATACA
 2521 AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT TTTCGGGTTT ATTACAGGGA
 2581 CAGCAGAGAT CCAGTTTGGT TAGTACCGGG CCCGCTCTAG AGATCCGACG CCGCCATCTC
 2641 TAGGCCCGCG CCGGCCCCCT CGCACAGACT TGTGGGAGAA GCTCGGCTAC TCCCCTGCCC
 2701 CGGTTAATTT GCATATAATA TTTCCTAGTA ACTATAGAGG CTTAATGTGC GATAAAAGAC
 2761 AGATAATCTG TTCTTTTTAA TACTAGCTAC ATTTTACATG ATAGGCTTGG ATTTCTATAA
 2821 GAGATACAAA TACTAAATTA TTATTTTAAA AAACAGCACA AAAGGAAACT CACCCTAACT
 2881 GTAAAGTAAT TATAACTTCG TATAGTATAA ATTATACGAA GTTATAAGCC TTGGTTTTTT
 2941 GAATTCCGTA TTACCGCCAT GCATTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT
 3001 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG
 3061 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC
 3121 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC
 3181 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG
 3241 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT
 3301 CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT TGGCAGTACA TCAATGGGCG
 3361 TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC CCCATTGACG TCAATGGGAG
 3421 TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT CGTAACAACT CCGCCCCATT
 3481 GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG CTGGTTTAGT
 3541 GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGCTG
 3601 TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC
 3661 AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC
 3721 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC
 3781 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC
 3841 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG
 3901 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC
 3961 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC
 4021 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC
 4081 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC
 4141 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG
 4201 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC
 4261 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAGCGGC CGCATAACTT CGTATAGTAT
 4321 AAATTATACG AAGTTATAAG CCTTGTTAAC GCGCGGTGAC CCTCGAGGTC GACGGTATCG
 4381 ATAAGCTCGC TTCACGAGAT CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA
 4441 TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC
 4501 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG
 4561 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC
 4621 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG
 4681 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG
 4741 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA
 4801 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT
 4861 TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG
 4921 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC
 4981 CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC
 5041 CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC
 5101 AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG
 5161 GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT
 5221 CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA
 5281 CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC
 5341 TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA
 5401 CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC
 5461 CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC
 5521 AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC
 5581 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG
 5641 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT
 5701 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA
 5761 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
 5821 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
 5881 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
 5941 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
 6001 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
 6061 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
 6121 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
 6181 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
 6241 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
 6301 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
 6361 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
 6421 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
 6481 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
 6541 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
 6601 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
 6661 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA
 6721 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
 6781 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
 6841 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
 6901 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
 6961 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
 7021 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
 7081 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
 7141 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
 7201 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
 7261 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
 7321 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
 7381 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
 7441 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
 7501 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
 7561 CCTGAC
//

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