| 质粒名称: | pLVX-EGFP-IRES-puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 128652 |
| 存储实验室: | Robert Sobol |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-GWE-IRES-puro |
| 空载体大小: | 8247bp |
| 完整质粒大小: | 8911 bp |
| 载体类型: | 哺乳细胞表达 、Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | EGFP |
| 插入大小: | 720bp |
| 基因突变: | Wild type |
| GenBank编号 | JNC_025025.1 |
| 启动子: | CMV |
| 标签/融合蛋白: | N/A |
| 克隆方法: | N/A |
| 5’测序引物 | M13 forward for pENTR vector |
| 3’测序引物 | M13 reverse for pENTR vector |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EGFP-IRES-puro was a gift from Robert Sobol (Addgene plasmid # 128652 ; http://n2t.net/addgene:128652 ; RRID:Addgene_128652) |
| 参考文献部分: | HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase beta. Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW. Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513. 10.1038/ncomms6513 PubMed 25423885 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EGFP-IRES-puro 8911 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mammalian expression of EGFP with puromycin selection.
ACCESSION .
VERSION .
KEYWORDS pLVX-EGFP-IRES-puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8911)
AUTHORS Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D,
Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
TITLE HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
polymerase beta.
JOURNAL Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
PUBMED 25423885
REFERENCE 2 (bases 1 to 8911)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8911
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2659..2679
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2705..2729
/label=LNCX
/note="Human CMV promoter, forward primer"
protein_bind 2824..2848
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2869..3588
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2869..3585
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2914..2935)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3175..3194)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3522..3543
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(3605..3629)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 3661..4234
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3828..3845)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4055..4074
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4267..4866
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4267..4286)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4763..4783
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4880..5468
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4933..4953)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5351..5362)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5675..6308
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6436..6452)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6436..6452)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6449..6471)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6460..6476
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6484..6514)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6529..6550
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6667..6684)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6838..7426)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6918..6937)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7597..8457)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8220..8239
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8458..8562)
/gene="bla"
/label=AmpR promoter
polyA_signal 8610..8744
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8647..8666)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8701..8720
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaga
2821 tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggtgagcaag
2881 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
2941 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
3001 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
3061 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
3121 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
3181 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
3241 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
3301 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
3361 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
3421 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
3481 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
3541 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaaa gggtgggcgc
3601 gccgacccag ctttcttgta caaagtggtt gatctagttc tagagcggcc gcggatcccg
3661 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
3721 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
3781 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
3841 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
3901 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
3961 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
4021 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
4081 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
4141 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
4201 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga
4261 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
4321 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
4381 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
4441 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
4501 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
4561 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
4621 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
4681 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
4741 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
4801 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
4861 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
4921 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
4981 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
5041 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
5101 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
5161 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
5221 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
5281 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
5341 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
5401 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
5461 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
5521 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
5581 gtgagttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
5641 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
5701 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
5761 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
5821 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
5881 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
5941 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
6001 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
6061 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
6121 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
6181 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
6241 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
6301 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
6361 aatatcagag agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag
6421 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
6481 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
6541 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
6601 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
6661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
6721 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
6781 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
6841 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
6901 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
6961 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
7021 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
7081 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
7141 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
7201 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
7261 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
7321 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
7381 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
7441 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
7501 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
7561 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
7621 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
7681 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
7741 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
7801 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
7861 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
7921 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
7981 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
8041 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
8101 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
8161 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
8221 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
8281 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
8341 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
8401 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
8461 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
8521 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
8581 gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca
8641 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
8701 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
8761 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
8821 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
8881 tttgttaaat atgtactaca aacttagtag t
//
