| 出品公司: | ATCC |
|---|---|
| 载体名称: | pSV2-Neo |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点，Hind III |
| 启动子: | SV40 Early |
| 载体大小: | 5.7 kb |
| 5' 测序引物及序列: | SV40pro-F：TATTTATGCAGAGGCCGAGG |
| 3' 测序引物及序列: | Neo-R: gcccagtcatagccgaatag |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | G418 |
| 克隆菌株: | DH5α ,HB101等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 产品目录号: | #37149 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pSV2-Neo载体 pSV2Neo质粒基本信息
pSV2-Neo质粒图谱 pSV2Neo载体图谱和pSV2-Neo载体序列 pSV2Neo质粒序列多克隆位点信息
pSV2-Neo质粒 pSV2Neo载体简介
pSV2-Neo质粒序列 pSV2Neo载体序列
LOCUS pSV2-Neo 5729 bp ds-DNA circular SYN 13-FEB-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSV2-Neo pSV2Neo
REFERENCE 1 (bases 1 to 5729)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, February 13, 2019 from Biofeng
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5729
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 752..886
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(1311..1331)
/codon_start=1
/product="nuclear localization signal of SV40
large T antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
intron 1461..1526
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS complement(1947..2741)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and
G418(Geneticin(R))"
promoter complement(3098..3427)
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3112..3247
/note="SV40 ori"
/note="SV40 origin of replication"
misc_feature 3578..3718
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(3904..4492)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
CDS complement(4663..5523)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin,
and related antibiotics"
promoter complement(5524..5628)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 aattcctttg cctaatttaa atgaggactt aacctgtgga aatattttga tgtgggaagc
61 tgttactgtt aaaactgagg ttattggggt aactgctatg ttaaacttgc attcagggac
121 acaaaaaact catgaaaatg gtgctggaaa acccattcaa gggtcaaatt ttcatttttt
181 tgctgttggt ggggaacctt tggagctgca gggtgtgtta gcaaactaca ggaccaaata
241 tcctgctcaa actgtaaccc caaaaaatgc tacagttgac agtcagcaga tgaacactga
301 ccacaaggct gttttggata aggataatgc ttatccagtg gagtgctggg ttcctgatcc
361 aagtaaaaat gaaaacacta gatattttgg aacctacaca ggtggggaaa atgtgcctcc
421 tgttttgcac attactaaca cagcaaccac agtgcttctt gatgagcagg gtgttgggcc
481 cttgtgcaaa gctgacagct tgtatgtttc tgctgttgac atttgtgggc tgtttaccaa
541 cacttctgga acacagcagt ggaagggact tcccagatat tttaaaatta cccttagaaa
601 gcggtctgtg aaaaacccct acccaatttc ctttttgtta agtgacctaa ttaacaggag
661 gacacagagg gtggatgggc agcctatgat tggaatgtcc tctcaagtag aggaggttag
721 ggtttatgag gacacagagg agcttcctgg ggatccagac atgataagat acattgatga
781 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
841 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
901 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
961 cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
1021 tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
1081 ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
1141 ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
1201 gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
1261 gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
1321 ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
1381 atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
1441 tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
1501 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
1561 aattatgtca caccacagaa gtaaggttcc ttcacaaaga tccgggacca aagcggccat
1621 cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc tgctggtttc
1681 ctggatgccg acggatttgc actgccggta gaactccgcg aggtcgtcca gcctcaggca
1741 gcagctgaac caactcgcga ggggatcgag cccggggtgg gcgaagaact ccagcatgag
1801 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
1861 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
1921 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
1981 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
2041 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
2101 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
2161 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
2221 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
2281 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
2341 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
2401 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
2461 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
2521 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
2581 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
2641 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
2701 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
2761 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
2821 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
2881 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
2941 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
3001 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
3061 cccttgcgcc ctgagtgctt gcggcagcgt gaagcttttt gcaaaagcct aggcctccaa
3121 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
3181 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
3241 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt
3301 gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga
3361 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact
3421 gacacacatt ccacagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
3481 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
3541 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
3601 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
3661 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
3721 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
3781 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
3841 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
3901 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
3961 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
4021 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
4081 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
4141 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
4201 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
4261 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
4321 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
4381 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
4441 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
4501 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
4561 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
4621 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
4681 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
4741 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
4801 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
4861 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
4921 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
4981 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
5041 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
5101 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
5161 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
5221 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
5281 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
5341 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
5401 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
5461 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
5521 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
5581 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
5641 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
5701 gcgtatcacg aggccctttc gtcttcaag
//
