pEG202

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pEG202
质粒类型: 酵母质粒
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 10166 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pEG202载体图谱

载体简介

载体序列

LOCUS       pEG202               10166 bp ds-DNA     circular SYN 19-OCT-2017
DEFINITION  Episomal yeast two-hybrid vector for constitutive expression of LexA
            fused to the N-terminus of a protein. Also known as pLexA.
ACCESSION   U89960
VERSION     .
KEYWORDS    pEG202
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Friday, February 14, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     promoter        1110..1511
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1538..2143
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /note="LexA"
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     misc_feature    2144..2182
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      2209..2396
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
     rep_origin      3128..4470
                     /note="2u ori"
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6114..6773)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /note="HIS3"
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6774..6961)
                     /gene="S. cerevisiae HIS3"
                     /note="HIS3 promoter"
     promoter        8026..8130
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             8131..8991
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9162..9750
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg
       61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa
      121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg
      181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg
      241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc
      301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc
      361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa
      421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg
      481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata
      541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag
      601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg
      661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa
      721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt
      781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac
      841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct
      901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata
      961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact
     1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc
     1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt
     1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac
     1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
     1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
     1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc
     1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg
     1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca
     1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca
     1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg
     1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa
     1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct
     1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact
     1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa
     1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg
     1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg
     1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt
     2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat
     2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattcc cggggatccg
     2161 tcgaccatgg cggccgctcg agtcgacctg cagccaagct aattccgggc gaatttctta
     2221 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     2281 agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt cctgtaggtc
     2341 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     2401 ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg ctaactccag
     2461 caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaaca cctgttgtaa
     2521 tcgttcttcc acacggatcg atccacagga cgggtgtggt cgccatgatc gcgtagtcga
     2581 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
     2641 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
     2701 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca
     2761 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
     2821 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
     2881 attaaagcta gctttgaaga aaaatgcgcc ttattcaatc tttgctataa aaaatggccc
     2941 aaaatctcac attggaagac atttgatgac ctcatttctt tcaatgaagg gcctaacgga
     3001 gttgactaat gttgtgggaa attggagcga taagcgtgct tctgccgtgg ccaggacaac
     3061 gtatactcat cagataacag caatacctga tcactacttc gcactagttt ctcggtacta
     3121 tgcatatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
     3181 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
     3241 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
     3301 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
     3361 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     3421 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     3481 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
     3541 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
     3601 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
     3661 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
     3721 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
     3781 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
     3841 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
     3901 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
     3961 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
     4021 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
     4081 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
     4141 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
     4201 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
     4261 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
     4321 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
     4381 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
     4441 ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa gtgcaagatg
     4501 gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa tagatgcaat
     4561 agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga ctatatatta
     4621 ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg gatgttcaaa
     4681 attcaatgat gggtaacaag tacgatcgta aatctgtaaa acagtttgtc ggatattagg
     4741 ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc tgcagcaggc gtgaagttag
     4801 acgacaactt ctctctggaa acgcataccg atattcaggc tgctgcaaag gcacaggcta
     4861 gtgcccgtgc gagtgcatcc ggtaccaccc cagatgctgt agtagcttct ggtagcactg
     4921 caatgagcca tgcttatcaa gaaaacacag gttttggtac tcgtcccata tatcttgaca
     4981 tgcaagccac tacaccaaca gaccctaggg ttttggatac gatgttgaag ttttatacgg
     5041 gactttatgg taatcctcat tccaacactc actcttacgg ttgggaaaca aatactgctg
     5101 tggaaaatgc tagagctcac gtagcaaaga tgatcaatgc cgaccccaag gaaataatat
     5161 tcacttcggg agcgaccgaa tctaataata tggttcttaa gggtgtccca agattttata
     5221 agaagactaa gaaacacatc atcaccacta gaacggaaca caagtgtgtc ttggaagccg
     5281 cacgggccat gatgaaggag ggatttgaag tcactttcct aaatgtggac gatcaaggtc
     5341 ttatcgattt gaaggaattg gaagatgcca ttagaccaga tacctgtctc gtctctgtga
     5401 tggctgtcaa taatgaaatc ggtgtcattc aacctattaa agaaattgga gcaatttgta
     5461 gaaagaataa gatcctcggg gacaccaaat atggcgatct cggccttttc gtttcttgga
     5521 gctgggacat gtttgccatc gatccatcta ccaccagaac ggccgttaga tctgctgcca
     5581 ccgttgtttc caccgaagaa accaccgttg ccgtaaccac cacgacggtt gttgctaaag
     5641 aagctgccac cgccacggcc accgttgtag ccgccgttgt tgttattgta gttgctactg
     5701 ttatttctgg cacttcttgg ttttcctctt aagtgaggag gaacataacc attctcgttg
     5761 ttgtcgttga tgcttaaatt ttgcacttgt tcgctcagtt cagccataat atgaaatgct
     5821 tttcttgttg ttcttacgga ataccacttg ccacctatca ccacaactaa ctttttcccg
     5881 ttcctccatc tcttttatat tttttttctc gatcgagttc aagagaaaaa aaaagaaaaa
     5941 gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta
     6001 ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac
     6061 atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata
     6121 agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg
     6181 gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga
     6241 caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc
     6301 atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac
     6361 ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct
     6421 gatccaaacc tttttactcc acgcgccagt agggcctctt taaaagcttg accgagagca
     6481 atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg
     6541 attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag aaaccctata
     6601 cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc ttctgcctct
     6661 ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc
     6721 tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc
     6781 ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat
     6841 ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc
     6901 taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata gagtgtacta
     6961 gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc
     7021 tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac caagctctta
     7081 aaacgagaat taagaaaaag tcgtcatctt tcgataagtt tttcccacag caaagcaata
     7141 gtagaaaaac aatgggaaac gttgaatgaa gacaaagcgt cgtggtttaa aaggaaatac
     7201 gctcacgtac atgctaggga acaggaccgt gcagcggatc taatgaatcc atttgttagt
     7261 taatagttta aatgttttta tcggaagagg ttttgtcatc acatcagcaa tgttcttctt
     7321 ggtctcgatg tagtatacgt ataaattatt acctgatact tcatctctaa gtctcattgc
     7381 ctttgtgcca aaaaatctgt ttctaaattt ctcttcattt gtagacttaa ttatactgat
     7441 cgttgatcta ctatcagtaa gtaagccttt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     7501 acctgtaaca atagcaatac cccaaatacc taatgtagtt ccagcaagca agctaaaaag
     7561 taaagcaaca acataactca cccctgcatc tgcagctttt gcccgggcag cctgctctgc
     7621 ctgtgttttc tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagcgtc
     7681 acatcggata ataatgatgg cagccattgt agaagtgcct tttgcatttc tagtctcttt
     7741 ctcggtctag ctagttttac tacatcgcga agatagaatc ttagatcaca ctgcctttgc
     7801 tgagctggat caatagagta acaaaagagt ggtaaggcct cgttaaagga caaggacctg
     7861 agcggaagtg tatcgtacag tagacggagt atactagtat agtctatagt ccgtggaatt
     7921 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8341 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     8401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8641 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     8701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9301 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9901 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     9961 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
    10021 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
    10081 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    10141 acagacaagc tgtgaccgtc tccggg
//

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