| 质粒名称: | pCBASceI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26477 |
| 存储实验室: | Maria Jasin |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGGS |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3073 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pCBASceI,CBAS |
| 物种来源: | -- |
| 插入大小: | 2583 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | HA（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI（克隆过程中销毁） |
| 3' 克隆位点: | XhoI（克隆过程中销毁） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCBASceI was a gift from Maria Jasin (Addgene plasmid # 26477 ; http://n2t.net/addgene:26477 ; RRID:Addgene_26477) |
| 参考文献部分: | Double-strand break repair by interchromosomal recombination: suppression of chromosomal translocations. Richardson C, Moynahan ME, Jasin M. Genes Dev. 1998 Dec 15. 12(24):3831-42. 10.1101/gad.12.24.3831 PubMed 9869637 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCBASceI 5665 bp ds-DNA circular SYN 19-APR-2019
DEFINITION I-SceI endonuclease expression vector with mammalian promoter to
introduce a DSB at a genomic I-SceI site.
ACCESSION .
VERSION .
KEYWORDS pCBASceI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5665)
AUTHORS Richardson C, Moynahan ME, Jasin M
TITLE Double-strand break repair by interchromosomal recombination:
suppression of chromosomal translocations.
JOURNAL Genes Dev. 1998 Dec 15. 12(24):3831-42.
PUBMED 9869637
REFERENCE 2 (bases 1 to 5665)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5665
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 5..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 661..1677
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1601..1623
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1685..1704
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1772..1792
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1814..1834
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1853..1879
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(2687..2706)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 2752..2807
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(2806..2825)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3168..3184)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3168..3184)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3181..3203)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3192..3208
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3216..3246)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3261..3282
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3341..3536
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3387..3522
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3449..3468
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 3542..3676
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3579..3598)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3633..3652
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3744..3761)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3915..4503)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3995..4014)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4674..5534)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5297..5316
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5535..5639)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg ggggggcgcg ccaggcgggg gggggggggg gggggggggg
541 gggggggggg ggcggggggg ggcggcggca gccaatcaga gcggcgcgct ccgaaagttt
601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgaaatt
1741 cagatccgcc gccactatgg gatcaagatc gccaaaaaag aagagaaagg tgccgaagaa
1801 gcatgcagca ccaccaaaaa aaaaacgaaa agtagaagac ccacgattta tgtacccata
1861 cgatgttcct gactatgcgg gtatgaaaaa catcaaaaaa aaccaggtaa tgaacctggg
1921 tccgaactct aaactgctga aagaatacaa atcccagctg atcgaactga acatcgaaca
1981 gttcgaagca ggtatcggtc tgatcctggg tgatgcttac atccgttctc gtgatgaagg
2041 taaaacctac tgtatgcagt tcgagtggaa aaacaaagca tacatggacc acgtatgtct
2101 gctgtacgat cagtgggtac tgtccccgcc gcacaaaaaa gaacgtgtta accacctggg
2161 taacctggta atcacctggg gcgcccagac tttcaaacac caagctttca acaaactggc
2221 taacctgttc atcgttaaca acaaaaaaac catcccgaac aacctggttg aaaactacct
2281 gaccccgatg tctctggcat actggttcat ggatgatggt ggtaaatggg attacaacaa
2341 aaactctacc aacaaatcga tcgtactgaa cacccagtct ttcactttcg aagaagtaga
2401 atacctggtt aagggtctgc gtaacaaatt ccaactgaac tgttacgtaa aaatcaacaa
2461 aaacaaaccg atcatctaca tcgattctat gtcttacctg atcttctaca acctgatcaa
2521 accgtacctg atcccgcaga tgatgtacaa actgccgaac actatctcct ccgaaacttt
2581 cctgaaataa taagtcgatc gaggaattca ctcctcaggt gcaggctgcc tatcagaagg
2641 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
2701 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
2761 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
2821 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
2881 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
2941 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
3001 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
3061 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
3121 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
3181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
3241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
3301 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
3361 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
3481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
3541 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3661 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
3721 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
3781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
3841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
3901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
3961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
4021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
4081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
4141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
4201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4321 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
4381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
4501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
4561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
4621 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
4681 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
4741 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
4801 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
4861 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
4921 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
4981 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
5041 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
5101 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
5161 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5221 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5281 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5341 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5401 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
5461 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
5521 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
5581 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
5641 acatttcccc gaaaagtgcc acctg
//
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