| 质粒名称: | pCS2-Cas9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 47322 |
| 存储实验室: | Alex Schier |
|
载体骨架基本信息 |
|
| 载体名称: | pCS2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4095 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，CRISPR；爪蟾和斑马鱼的表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | -- |
| 插入大小: | 4100 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | SP6 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | ClaI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | SP6 |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2-Cas9 was a gift from Alex Schier (Addgene plasmid # 47322 ; http://n2t.net/addgene:47322 ; RRID:Addgene_47322) |
| 参考文献部分: | Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF. PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014. PONE-D-14-14210 [pii] PubMed 24873830 |
买家导航
pCS2-Cas9载体 pCS2Cas9质粒基本信息
pCS2-Cas9质粒图谱 pCS2Cas9载体图谱和pCS2-Cas9载体序列 pCS2Cas9质粒序列多克隆位点信息
pCS2-Cas9质粒 pCS2Cas9载体简介
pCS2-Cas9质粒序列 pCS2Cas9载体序列
LOCUS pCS2-Cas9 8215 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION For in vivo expression of Cas9 or generation of Cas9 mRNA.
ACCESSION .
VERSION .
KEYWORDS pCS2-Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8215)
AUTHORS Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A,
Montague TG, Zimmerman S, Richter C, Schier AF
TITLE Efficient mutagenesis by Cas9 protein-mediated oligonucleotide
insertion and large-scale assessment of single-guide RNAs.
JOURNAL PLoS One. 2014 May 29;9(5):e98186. doi:
10.1371/journal.pone.0098186. eCollection 2014.
PUBMED 24873830
REFERENCE 2 (bases 1 to 8215)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8215
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 240..260
/label=T3
/note="T3 promoter, forward primer"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 373..507
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(410..429)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 464..483
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 512..529
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(540..560)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(573..4676)
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
regulatory 4673..4682
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(4721..4739)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4722..4739)
/label=SP6
/note="SP6 promoter, forward primer"
promoter complement(4774..5751)
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
rep_origin 5829..6284
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5916..5935)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6126..6147
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6310..6414
/gene="bla"
/label=AmpR promoter
CDS 6415..7275
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6633..6652)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7446..8034
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7935..7954
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8188..8205
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
241 caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gcccaatgca ttggcgccgc
301 ggccgcgaat taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
361 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
481 atcaatgtat cttatcatgt ctggatctac gtaatacgac tcactatagt tctagattac
541 accttcctct tcttcttggg gtcagccctg ctgtctccac cgagctgaga gaggtcgatt
601 cttgtttcat agagccccgt aattgactga tgaatcagtg tggcgtccag gacctccttt
661 gtagaggtgt accgctttct gtctatggtg gtgtcgaagt acttgaaggc tgcaggcgcg
721 cccaagttgg tcagagtaaa caagtggata atgttttctg cctgctccct gatgggctta
781 tccctgtgct tattgtaagc agaaagcacc ttatcgaggt tagcgtcggc gaggatcact
841 cttttggaga attcgcttat ttgctcgatg atctcatcaa ggtagtgttt gtgttgttcc
901 acgaacagct gcttctgctc attatcttcg ggagaccctt tgagcttttc atagtggctg
961 gccagataca agaaattaac gtatttagag ggcagtgcca gctcgttacc tttctgcagc
1021 tcgcccgcac tagcgagcat tcgtttccgg ccgttttcaa gctcaaagag agagtacttg
1081 ggaagcttaa tgatgaggtc ttttttgacc tctttatatc ctttcgcctc gagaaagtcg
1141 atggggtttt tttcgaagct tgatcgctcc atgattgtga tgcccagcag ttccttgacg
1201 cttttgagtt ttttagactt ccctttctcc actttggcca caaccagtac actgtaagcg
1261 actgtaggag aatcgaatcc gccgtatttc ttggggtccc aatctttttt gcgtgcgatc
1321 agcttgtcgc tgttcctttt cgggaggata ctttccttgg agaagcctcc ggtctgtact
1381 tcggtctttt taacgatgtt cacctgcggc atggacagga ccttccggac tgtcgcgaaa
1441 tccctaccct tgtcccacac gatttctcct gtttctccgt ttgtttcgat aagtggtcgc
1501 ttccgaatct ctccattggc cagtgtaatc tcggtcttga aaaaattcat aatattgctg
1561 taaaagaagt acttagcggt ggccttgcct atttcctgct cagactttgc gatcattttc
1621 ctaacatcgt acactttata gtctccgtaa acaaattcag attcaagctt gggatatttt
1681 ttgataagtg cagtgcctac cactgcattc aggtaggcat catgcgcatg gtggtaattg
1741 ttgatctctc tcaccttata aaactgaaag tcctttctga aatctgagac cagcttagac
1801 ttcagagtaa taactttcac ctctcgaatc agtttgtcat tttcatcgta cttggtgttc
1861 atgcgtgaat cgagaatttg ggccacgtgc ttggtgatct ggcgtgtctc aacaagctgc
1921 cttttgatga agccggcttt atccaactca gacaggccac ctcgttcagc cttagtcaga
1981 ttatcgaact tccgttgtgt gatcagtttg gcgttcagca gctgccgcca ataatttttc
2041 attttcttga caacttcttc tgaggggacg ttatcactct tccctctatt tttatcggat
2101 cttgtcaaca ctttattatc aatagaatca tctttgagaa aagactgggg cacgatatga
2161 tccacgtcgt agtcggagag ccgattgatg tccagttcct gatccacgta catgtccctg
2221 ccgttctgca ggtagtacag gtagagcttc tcattctgaa gctgggtgtt ttcaactggg
2281 tgttccttaa ggatttggga ccccagttct tttataccct cttcaatcct cttcatcctt
2341 tccctactgt tcttctgtcc cttctgggta gtttggttct ctcgggccat ctcgataacg
2401 atattctcgg gcttatgcct tcccattact ttgacgagtt catccacgac cttaacggtc
2461 tgcagtattc cctttttgat agctgggcta cctgcaagat tagcgatgtg ctcgtgaaga
2521 ctgtccccct ggccagaaac ttgtgctttc tggatgtcct ccttaaaggt gagagagtca
2581 tcatggatca actgcatgaa gttccggttg gcaaatccat cggacttaag aaaatccagg
2641 attgtctttc cactctgctt gtctcggatc ccattgatca gttttcttga cagccgcccc
2701 catcctgtat atcggcgcct cttgagctgt ttcatgactt tgtcgtcgaa gagatgagcg
2761 taagttttca agcgttcttc aatcatctcc ctatcttcaa acaacgtaag ggtgaggaca
2821 atgtcctcaa gaatgtcctc gttctcctca ttgtccagga agtccttgtc tttaatgatt
2881 ttcaggagat cgtgatacgt tcccagggat gcgttgaagc gatcctccac tccgctgatt
2941 tcaacagagt cgaaacattc aatctttttg aaatagtctt ctttgagctg tttcacggta
3001 actttccggt tcgtcttgaa gaggaggtcc acgatagctt tcttctgctc tccagacagg
3061 aatgctggct ttctcatccc ttctgtgacg tatttgacct tggtgagctc gttataaact
3121 gtgaagtact cgtacagcag agagtgttta ggaagcacct tttcgttagg cagattttta
3181 tcaaagttag tcatcctttc gatgaaggac tgggcagagg cccccttatc cacgacttcc
3241 tcgaagttcc agggagtgat ggtctcttct gatttgcgag tcatccacgc gaatctggaa
3301 tttccccggg cgagggggcc tacatagtag ggtatccgaa atgtgaggat tttctcaatc
3361 ttttccctgt tatctttcaa aaaggggtag aaatcctctt gccgcctgag gatagcgtgc
3421 agttcgccca ggtgaatctg gtgggggatg cttccattgt cgaaagtgcg ctgtttgcgc
3481 aacagatctt ctctgttaag ctttaccagc agctcctcgg tgccgtccat tttttccaag
3541 atgggcttaa taaatttgta aaattcctcc tggcttgctc cgccgtcaat gtatccggcg
3601 tagccatttt tagactgatc gaagaaaatt tccttgtact tctcaggcag ttgctgtctg
3661 acaagggcct tcagcaaagt caagtcttgg tggtgctcat catagcgctt gatcatacta
3721 gcgctcagcg gagctttggt gatctccgtg ttcactcgca gaatatcact cagcagaatg
3781 gcgtctgaca ggttctttgc cgccaaaaaa aggtctgcgt actggtcgcc gatctgggcc
3841 agcagattgt cgagatcatc atcgtaggtg tctttgctca gttgaagctt ggcatcttcg
3901 gccaggtcga agttagattt aaagttgggg gtcagcccga gtgacagggc gataagatta
3961 ccaaacaggc cgttcttctt ctccccaggg agctgtgcga tgaggttttc gagccgccgg
4021 gatttggaca gcctagcgct caggattgct ttggcgtcaa ctccggatgc gttgatcggg
4081 ttctcttcga aaagctgatt gtaagtctga accagttgga taaagagttt gtcgacatcg
4141 ctgttgtctg ggttcaggtc cccctcgatg aggaagtgtc cccgaaattt gatcatatgc
4201 gccagcgcga gatagatcaa ccgcaagtca gccttatcag tactgtctac aagcttcttc
4261 ctcagatgat atatggttgg gtacttttca tggtacgcca cctcgtccac gatattgcca
4321 aagattgggt ggcgctcgtg ctttttatcc tcctccacca aaaaggactc ctccagccta
4381 tggaagaaag agtcatccac cttagccatc tcattactaa agatctcctg caggtagcag
4441 atccgattct ttctgcgggt atatctgcgc cgtgctgttc ttttgagccg cgtggcttcg
4501 gccgtctccc cggagtcgaa caggagggcg ccaatgaggt tcttctttat gctgtggcga
4561 tcggtattgc ccagaacttt gaattttttg ctcggcacct tgtactcgtc cgtaatgacg
4621 gcccagccga cgctgtttgt gccgatatcg agcccaatgg agtacttctt gtccatggtg
4681 gcatcgatgg gatcctgcaa aaagaacaag tagcttgtat tctatagtgt cacctaaatc
4741 aagcttgctc cgacgtcccc aggcagaatg gcggttccct aaacgagcat tgcttatata
4801 gacctcccat taggcacgcc taccgcccat ttacgtcaat ggaacgccca tttgcgtcat
4861 tgcccctccc cattgacgtc aatggggatg tacttggcag ccatcgcggg ccatttaccg
4921 ccattgacgt caatgggagt actgccaatg taccctggcg tacttccaat agtaatgtac
4981 ttgccaagtt actattaata gatattgatg tactgccaag tgggccattt accgtcattg
5041 acgtcaatag ggggcgtgag aacggatatg aatgggcaat gagccatccc attgacgtca
5101 atggtgggtg gtcctattga cgtcaatggg cattgagcca ggcgggccat ttaccgtaat
5161 tgacgtcaat gggggaggcg ccatatacgt caataggacc gcccatatga cgtcaatagg
5221 aaagaccata tatagagacc attgacgtca atgggggagt ggctatgggc ggtattagga
5281 agccccatat atggtatatg ggaccgccca ttgggagggg ctatctacgt caataggaaa
5341 acccatatat ggaatactat atggcatagg gccaatacat agtattgaac ctggccaata
5401 gccatattgg catagggcca tattggatat tgcctatata ttgatcctgg catatagcca
5461 atatggccgc cattattggc accatgccaa ttcaatatgg cggacttggc acagggccaa
5521 gacccctcct caaacttggc accgtgccaa gtagacccct ccccagttgg cacagtgcca
5581 ggtccgccat attgaattgg catggtgcca agtccgccat attgaattgg ctggggtcca
5641 gatccaccat attgaattgg cacgagtcca tatacgccat attgaattgg cacaggtcca
5701 gatccaccat attgaattgg catgagtcca tatacgccat attgaattgg cggtcgactg
5761 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
5821 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
5881 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
5941 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
6001 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
6061 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
6121 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
6181 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
6241 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
6301 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
6361 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
6421 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
6481 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
6541 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
6601 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
6661 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
6721 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
6781 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
6841 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
6901 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
6961 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
7021 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
7081 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
7141 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
7201 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
7261 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
7321 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
7381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
7441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
7501 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
7561 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
7621 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
7681 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
7741 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
7801 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
7861 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
7921 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
7981 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
8041 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
8101 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
8161 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
