pLVX-Hom-Mem1

价格:4000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-Hom-Mem1
质粒类型: 慢病毒载体;双顺反子载体;信号通路载体;亚细胞定位载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 8411 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: DmrB
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-Hom-Mem1表达DmrB融合蛋白;利用信号受体的二聚化作用研究细胞信号通路;DmrB蛋白加了膜定位信号。
产品目录号: 635073
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-Hom-Mem1载体图谱



pLVX-Hom-Mem1 多克隆位点

pLVX-Hom-Mem1 载体特征

载体简介

注:
When cloning your gene of interest into the pLVX-Hom-Mem1 vector, make certain that the N-myr-signal is located on the N-terminus of the DmrB fusion protein. The N‐myr‐signal is NOT functional if localized in the middle or at the C‐terminus of a protein of interest. Your gene must either be cloned into the 3’MCS, or if cloned into the 5’ MCS, which is located upstream of the N‐myr signal, you must include a separate N‐myr signal sequence on the forward primer used to amplify your gene of interest.

载体序列

LOCUS       pLVX-Hom-Mem1          8841 bp ds-DNA    circular SYN 30-JUL-2013
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
KEYWORDS    pLVX-Hom-Mem1 Vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Clontech Laboratories Inc.

FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label="5' LTR"                     
     misc_feature    681..806
                     /label="Packaging signal"
     misc_feature    1303..1536
                     /label="RRE"
     misc_feature    2028..2144
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /label="CMV enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
     misc_feature    2803..2820
                     /label="5' MCS"
     misc_feature    2821..2862
                     /label="N-myr signal"
     CDS             2869..3189
                     /label="DmrB"
     CDS             3196..3516
                     /label="DmrB"
     misc_feature    3518..3531
                     /label="3' MCS"
     misc_feature    3544..4117
                     /label="IRES"
     CDS             4150..4749
                     /label="PuroR"
     misc_feature    4763..5351
                     /label="WPRE"
     LTR             5559..6192
                     /label="3' LTR"
     rep_origin      complement(6723..7308)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7479..8339)
                     /label="AmpR"
     promoter        complement(8340..8444)
                     /label="AmpR promoter"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg cgtccaagtc
     2881 gaaaccatta gtcccggcga tggcagaaca tttcctaaaa ggggacaaac atgtgtcgtc
     2941 cattatacag gcatgttgga ggacggcaaa aaggtggaca gtagtagaga tcgcaataaa
     3001 cctttcaaat tcatgttggg aaaacaagaa gtcattaggg gatgggagga gggcgtggct
     3061 caaatgtccg tcggccaacg cgctaagctc accatcagcc ccgactacgc atacggcgct
     3121 accggacatc ccggaattat tccccctcac gctaccttgg tgtttgacgt cgaactgttg
     3181 aagctcgaga ctagaggagt gcaggtggag actatctccc caggagacgg gcgcaccttc
     3241 cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa
     3301 gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa gcaggaggtg
     3361 atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc caaactgact
     3421 atatctccag attatgccta tggtgccact gggcacccag gcatcatccc accacatgcc
     3481 actctcgtct tcgatgtgga gcttctaaaa ctggaaagcg gccgcggatc ctacgtatga
     3541 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     3601 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3661 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3721 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3781 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     3841 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     3901 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3961 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4021 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4081 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga
     4141 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4201 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     4261 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     4321 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     4381 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     4441 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     4501 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     4561 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     4621 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     4681 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     4741 ggtgcctaga cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     4801 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4861 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4921 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4981 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5041 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5101 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     5161 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5221 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5281 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5341 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     5401 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     5461 gaggtgggtt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5521 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     5581 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     5641 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     5701 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     5761 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     5821 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     5881 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     5941 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     6001 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6061 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6121 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6181 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6241 aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc
     6301 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     8101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8461 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
     8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     8641 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     8701 agtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     8761 gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat
     8821 atgtactaca aacttagtag t
//

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