| 质粒名称: | MSCV-IRES-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20672 |
| 存储实验室: | Tannishtha Reya |
|
载体骨架基本信息 |
|
| 载体名称: | MSCV |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6500 bp |
| 载体类型: | 哺乳细胞表达，Retroviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 标签/融合蛋白: | IRES EGFP (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | pLXSN5; pBABE5 |
| 3' 测序引物 | pCDH-rev (GCATTCCTTTGGCGAGAG) |
|
文献引用方法 |
|
| 材料和方法部分: | MSCV-IRES-GFP was a gift from Tannishtha Reya (爱迪基因 plasmid # 20672) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MSCV-IRES-GFP 6488 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS MSCV-IRES-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6488)
TITLE Reya 2009
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|MSCV-IRES-GFP|
FEATURES Location/Qualifiers
source 1..6488
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(455..1315)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1078..1097
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1316..1420)
/gene="bla"
/label=AmpR promoter
primer_bind 1488..1506
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(1544..1566)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 1666..1685
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1879..1901
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2029..2048
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 2248..2764
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 2828..3169
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 3236..3652
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 3582..3604
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 3620..3636
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(3841..3858)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4068..4087
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4249..4968
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4294..4315)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4555..4574)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4902..4923
/label=EGFP-C
/note="EGFP, forward primer"
LTR 5130..5644
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 5806..5822
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5830..5860)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5875..5896
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6013..6030)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6184..284)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6264..6283)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
61 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
1021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
1081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
1141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
1201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
1261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
1321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
1381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
1441 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
1501 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
1561 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
1621 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
1681 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
1741 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
1801 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
1861 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
1921 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
1981 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
2041 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
2101 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
2161 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
2221 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
2281 agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc
2341 agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa
2401 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc
2461 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
2521 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
2581 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
2641 gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg
2701 actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc gggggtcttt
2761 catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga cccccccgcc
2821 gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc gtgtttgtgc
2881 cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
2941 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
3001 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
3061 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
3121 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
3181 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
3241 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
3301 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
3361 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
3421 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
3481 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
3541 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
3601 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcg
3661 ttaacctcga gcgggatcaa ttccgccccc cccctaacgt tactggccga agccgcttgg
3721 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
3781 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
3841 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
3901 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
3961 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
4021 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
4081 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
4141 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
4201 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat ggtgagcaag
4261 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
4321 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
4381 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
4441 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
4501 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
4561 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
4621 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
4681 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
4741 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
4801 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
4861 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
4921 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
4981 tctagagtcg acctgcaggc atgcaagctt caggtagccg gctaacgtta acaaccggta
5041 cctctagaac tatagctagc atgcgcaaat ttaaagcgct gatatcgata aaataaaaga
5101 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
5161 tagcttaagt aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt
5221 cagatcaagg ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta
5281 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct
5341 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
5401 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
5461 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac
5521 tgcgtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
5581 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
5641 tcatgggtaa cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt
5701 aagtaatcct gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat
5761 agttcattat ggacagcgca gaaagagctg gggagaattg tgaaattgtt atccgctcac
5821 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
5881 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
5941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
6001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
6061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
6121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
6181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
6241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
6301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
6361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
6421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
6481 taactatc
//
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