pKD20

价格:1500元

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

出品公司: --
载体名称: pKD20
质粒类型: 细菌表达;基因敲除系统
高拷贝/低拷贝: --
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6078  bp
5' 测序引物及序列: --
3' 测序引物及序列: --
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产品目录号: --
稳定性: --
组成型: --
病毒/非病毒: --

载体图谱质粒图谱和多克隆位点信息

pKD20载体图谱



载体简介

载体序列

LOCUS       AY048745                6078 bp    DNA     circular SYN 11-SEP-2001
DEFINITION  Red recombinase plasmid pKD20, complete sequence.
ACCESSION   AY048745
VERSION     AY048745.1  GI:15554338
KEYWORDS    .
SOURCE      Red recombinase plasmid pKD20
  ORGANISM  Red recombinase plasmid pKD20
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Datsenko,K.A. and Wanner,B.L.
  TITLE     One-step inactivation of chromosomal genes in Escherichia coli K-12
            using PCR products
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000)
   PUBMED   10829079
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Datsenko,K.A. and Wanner,B.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2001) Biological Sciences, Purdue University,
            Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Red recombinase plasmid pKD20"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:170494"
     CDS             complement(11..889)
                     /codon_start=1
                     /transl_table=11
                     /product="araC protein"
                     /protein_id="AAL02039.1"
                     /db_xref="GI:15554339"

     misc_feature    complement(1040..1068)
                     /note="Pc"
     misc_feature    1165..1192
                     /note="ParaB"
     CDS             1244..1660
                     /note="recombination;138"
                     /codon_start=1
                     /transl_table=11
                     /product="gam protein"
                     /protein_id="AAL02040.1"
                     /db_xref="GI:15554340"

     CDS             1666..2451
                     /note="recombination;261"
                     /codon_start=1
                     /transl_table=11
                     /product="bet protein"
                     /protein_id="AAL02041.1"
                     /db_xref="GI:15554341"

     CDS             2448..3128
                     /note="exo"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="AAL02042.1"
                     /db_xref="GI:15554342"

     CDS             complement(3245..4195)
                     /codon_start=1
                     /transl_table=11
                     /product="repA101 protein"
                     /protein_id="AAL02043.1"
                     /db_xref="GI:15554343"

     rep_origin      4199..4939
                     /note="oriR101"
     CDS             complement(5087..5947)
                     /note="bla; ampicillin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAL02044.1"
                     /db_xref="GI:15554344"

ORIGIN      
        1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
       61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
      841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
      901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
      961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
     1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
     1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
     1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
     1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
     1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
     1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
     1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
     1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
     1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
     1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
     1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
     1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
     1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
     2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
     2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
     2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
     2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
     2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
     2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
     2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
     2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
     2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
     2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
     2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
     2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
     2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
     2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
     2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
     2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
     3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
     3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
     3121 ggcgatgata agctagccca tgggtatgga cagttttccc tttgatatgt aacggtgaac
     3181 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     3241 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     3301 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     3361 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     3421 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     3481 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     3541 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     3601 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     3661 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     3721 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     3781 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     3841 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     3901 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     3961 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     4021 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     4081 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     4141 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     4201 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     4261 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     4321 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     4381 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     4441 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     4501 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     4561 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc
     4621 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     4681 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     4741 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     4801 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     4861 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     4921 aggcagcggt atcatcaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6061 cccgaaaagt gccacctg
//

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